Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1149 and Pf1N1B4_1150 are separated by 290 nucleotides Pf1N1B4_1150 and Pf1N1B4_1151 are separated by 2 nucleotides Pf1N1B4_1151 and Pf1N1B4_1152 are separated by 64 nucleotides
Pf1N1B4_1149 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,294,529 to 1,296,559
_1149
Pf1N1B4_1150 - AmpE protein, at 1,296,850 to 1,297,686
_1150
Pf1N1B4_1151 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 1,297,689 to 1,298,258
_1151
Pf1N1B4_1152 - Thymidine phosphorylase (EC 2.4.2.4), at 1,298,323 to 1,300,524
_1152
Position (kb)
1296
1297
1298 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1295.946 kb on + strand, within Pf1N1B4_1149 at 1296.447 kb on + strand at 1296.447 kb on + strand at 1296.447 kb on + strand at 1296.447 kb on + strand at 1296.448 kb on - strand at 1296.448 kb on - strand at 1296.448 kb on - strand at 1296.544 kb on - strand at 1296.585 kb on - strand at 1296.590 kb on + strand at 1296.632 kb on + strand at 1296.640 kb on + strand at 1296.641 kb on - strand at 1296.641 kb on - strand at 1296.641 kb on - strand at 1296.643 kb on - strand at 1296.647 kb on + strand at 1296.648 kb on - strand at 1296.648 kb on - strand at 1296.648 kb on - strand at 1296.667 kb on + strand at 1296.668 kb on - strand at 1296.668 kb on - strand at 1296.672 kb on + strand at 1296.672 kb on + strand at 1296.672 kb on + strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.673 kb on - strand at 1296.758 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.774 kb on + strand at 1296.775 kb on - strand at 1296.775 kb on - strand at 1296.775 kb on - strand at 1296.851 kb on + strand at 1296.852 kb on - strand at 1297.260 kb on - strand at 1297.260 kb on - strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.323 kb on + strand, within Pf1N1B4_1150 at 1297.324 kb on - strand, within Pf1N1B4_1150 at 1297.324 kb on - strand, within Pf1N1B4_1150 at 1297.430 kb on + strand, within Pf1N1B4_1150 at 1297.430 kb on + strand, within Pf1N1B4_1150 at 1297.431 kb on - strand, within Pf1N1B4_1150 at 1297.431 kb on - strand, within Pf1N1B4_1150 at 1297.472 kb on + strand, within Pf1N1B4_1150 at 1297.473 kb on - strand, within Pf1N1B4_1150 at 1297.473 kb on - strand, within Pf1N1B4_1150 at 1297.691 kb on - strand at 1297.691 kb on - strand at 1298.069 kb on - strand, within Pf1N1B4_1151 at 1298.489 kb on + strand at 1298.489 kb on + strand at 1298.489 kb on + strand at 1298.489 kb on + strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand at 1298.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 1,295,946 + Pf1N1B4_1149 0.70 -0.5 1,296,447 + -0.8 1,296,447 + -0.8 1,296,447 + -1.0 1,296,447 + +0.2 1,296,448 - -1.3 1,296,448 - -0.5 1,296,448 - +0.2 1,296,544 - +1.5 1,296,585 - +0.2 1,296,590 + +0.5 1,296,632 + -0.2 1,296,640 + -0.1 1,296,641 - +0.2 1,296,641 - +0.8 1,296,641 - +0.5 1,296,643 - +0.5 1,296,647 + +1.1 1,296,648 - +0.5 1,296,648 - +0.3 1,296,648 - -0.4 1,296,667 + +0.8 1,296,668 - +0.3 1,296,668 - +0.4 1,296,672 + +2.2 1,296,672 + +0.5 1,296,672 + +0.9 1,296,673 - +0.5 1,296,673 - +0.5 1,296,673 - +1.5 1,296,673 - +0.8 1,296,673 - -0.9 1,296,758 + -0.7 1,296,774 + -0.5 1,296,774 + -0.1 1,296,774 + -0.2 1,296,774 + -0.8 1,296,774 + -0.3 1,296,775 - -0.3 1,296,775 - +0.5 1,296,775 - +0.5 1,296,851 + +0.9 1,296,852 - -0.1 1,297,260 - -0.1 1,297,260 - Pf1N1B4_1150 0.49 -0.3 1,297,323 + Pf1N1B4_1150 0.57 -1.3 1,297,323 + Pf1N1B4_1150 0.57 -1.1 1,297,323 + Pf1N1B4_1150 0.57 -0.3 1,297,323 + Pf1N1B4_1150 0.57 -1.5 1,297,324 - Pf1N1B4_1150 0.57 +1.5 1,297,324 - Pf1N1B4_1150 0.57 +1.8 1,297,430 + Pf1N1B4_1150 0.69 +0.9 1,297,430 + Pf1N1B4_1150 0.69 -1.1 1,297,431 - Pf1N1B4_1150 0.69 -1.3 1,297,431 - Pf1N1B4_1150 0.69 +0.9 1,297,472 + Pf1N1B4_1150 0.74 +1.1 1,297,473 - Pf1N1B4_1150 0.74 +1.8 1,297,473 - Pf1N1B4_1150 0.74 +0.5 1,297,691 - -0.7 1,297,691 - +0.1 1,298,069 - Pf1N1B4_1151 0.67 +0.9 1,298,489 + +0.5 1,298,489 + +0.8 1,298,489 + +0.3 1,298,489 + -1.5 1,298,490 - -0.9 1,298,490 - -0.7 1,298,490 - +0.5 1,298,490 - -0.2 1,298,490 - +0.2 1,298,490 - -0.4 1,298,490 - -0.2 1,298,490 - +0.5 1,298,490 - +3.1
Or see this region's nucleotide sequence