Experiment: Polymyxin B sulfate 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22180 and BPHYT_RS22185 are separated by 56 nucleotides BPHYT_RS22185 and BPHYT_RS22190 are separated by 603 nucleotides BPHYT_RS22190 and BPHYT_RS22195 are separated by 22 nucleotides BPHYT_RS22195 and BPHYT_RS22200 are separated by 146 nucleotides
BPHYT_RS22180: BPHYT_RS22180 - hypothetical protein, at 536,031 to 536,270
_RS22180
BPHYT_RS22185: BPHYT_RS22185 - hypothetical protein, at 536,327 to 536,617
_RS22185
BPHYT_RS22190: BPHYT_RS22190 - nucleoid-structuring protein H-NS, at 537,221 to 538,072
_RS22190
BPHYT_RS22195: BPHYT_RS22195 - hypothetical protein, at 538,095 to 538,580
_RS22195
BPHYT_RS22200: BPHYT_RS22200 - cardiolipin synthetase, at 538,727 to 539,989
_RS22200
Position (kb)
537
538
539 Strain fitness (log2 ratio)
-1
0
1 at 536.257 kb on + strand at 536.258 kb on - strand at 536.258 kb on - strand at 536.313 kb on + strand at 536.316 kb on - strand at 536.487 kb on + strand, within BPHYT_RS22185 at 536.718 kb on - strand at 536.853 kb on + strand at 536.920 kb on + strand at 536.922 kb on + strand at 536.936 kb on + strand at 537.452 kb on + strand, within BPHYT_RS22190 at 537.916 kb on - strand, within BPHYT_RS22190 at 538.093 kb on - strand at 538.310 kb on - strand, within BPHYT_RS22195 at 538.414 kb on + strand, within BPHYT_RS22195 at 538.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0625 mg/ml remove 536,257 + +0.0 536,258 - +0.2 536,258 - -0.1 536,313 + -0.4 536,316 - -0.1 536,487 + BPHYT_RS22185 0.55 +0.2 536,718 - -0.2 536,853 + -0.7 536,920 + +0.2 536,922 + -0.0 536,936 + -0.2 537,452 + BPHYT_RS22190 0.27 -1.0 537,916 - BPHYT_RS22190 0.82 -0.6 538,093 - -0.0 538,310 - BPHYT_RS22195 0.44 +0.2 538,414 + BPHYT_RS22195 0.66 +0.4 538,833 - -0.1
Or see this region's nucleotide sequence