Experiment: Polymyxin B sulfate 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21330 and BPHYT_RS21335 are separated by 246 nucleotides BPHYT_RS21335 and BPHYT_RS21340 overlap by 4 nucleotides BPHYT_RS21340 and BPHYT_RS21345 overlap by 4 nucleotides
BPHYT_RS21330: BPHYT_RS21330 - hypothetical protein, at 345,059 to 345,493
_RS21330
BPHYT_RS21335: BPHYT_RS21335 - CDP-alcohol phosphatidyltransferase, at 345,740 to 346,441
_RS21335
BPHYT_RS21340: BPHYT_RS21340 - 2OG-Fe(II) oxygenase, at 346,438 to 347,250
_RS21340
BPHYT_RS21345: BPHYT_RS21345 - membrane protein, at 347,247 to 348,266
_RS21345
Position (kb) 346
347
348 Strain fitness (log2 ratio) -2
-1
0
1 at 345.494 kb on - strand at 345.521 kb on + strand at 345.521 kb on + strand at 345.522 kb on - strand at 345.612 kb on + strand at 345.612 kb on + strand at 345.612 kb on + strand at 345.654 kb on - strand at 345.724 kb on + strand at 345.724 kb on + strand at 345.724 kb on + strand at 345.727 kb on - strand at 345.727 kb on - strand at 345.728 kb on + strand at 345.728 kb on + strand at 345.728 kb on + strand at 345.970 kb on + strand, within BPHYT_RS21335 at 346.443 kb on + strand at 346.482 kb on - strand at 346.482 kb on - strand at 346.725 kb on + strand, within BPHYT_RS21340 at 346.726 kb on - strand, within BPHYT_RS21340 at 346.855 kb on + strand, within BPHYT_RS21340 at 346.858 kb on + strand, within BPHYT_RS21340 at 346.858 kb on + strand, within BPHYT_RS21340 at 346.859 kb on - strand, within BPHYT_RS21340 at 346.899 kb on + strand, within BPHYT_RS21340 at 346.927 kb on - strand, within BPHYT_RS21340
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0625 mg/ml remove 345,494 - +0.1 345,521 + -0.8 345,521 + -0.1 345,522 - -0.2 345,612 + -0.1 345,612 + +0.0 345,612 + -0.2 345,654 - -2.2 345,724 + +0.4 345,724 + -0.5 345,724 + -0.3 345,727 - -1.0 345,727 - -1.3 345,728 + -2.0 345,728 + -0.0 345,728 + -0.3 345,970 + BPHYT_RS21335 0.33 -1.1 346,443 + +0.3 346,482 - -0.7 346,482 - +0.5 346,725 + BPHYT_RS21340 0.35 -0.4 346,726 - BPHYT_RS21340 0.35 -0.9 346,855 + BPHYT_RS21340 0.51 -1.3 346,858 + BPHYT_RS21340 0.52 -2.4 346,858 + BPHYT_RS21340 0.52 -0.7 346,859 - BPHYT_RS21340 0.52 -1.4 346,899 + BPHYT_RS21340 0.57 -1.5 346,927 - BPHYT_RS21340 0.60 +0.9
Or see this region's nucleotide sequence