Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_66

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_65 and Pf1N1B4_66 are separated by 61 nucleotidesPf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotidesPf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotides Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755 _65 Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353 _66 Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186 _67 Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535 _68 Position (kb) 68 69 70Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 67.838 kb on + strandat 67.838 kb on + strandat 67.839 kb on - strandat 67.839 kb on - strandat 67.923 kb on + strandat 67.923 kb on + strandat 67.923 kb on + strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.924 kb on - strandat 67.927 kb on - strandat 67.927 kb on - strandat 68.011 kb on - strand, within Pf1N1B4_65at 68.011 kb on - strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.019 kb on + strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.020 kb on - strand, within Pf1N1B4_65at 68.122 kb on - strand, within Pf1N1B4_65at 68.122 kb on - strand, within Pf1N1B4_65at 68.232 kb on - strand, within Pf1N1B4_65at 68.245 kb on + strand, within Pf1N1B4_65at 68.246 kb on - strandat 68.292 kb on + strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.293 kb on - strand, within Pf1N1B4_65at 68.484 kb on + strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.485 kb on - strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strandat 68.620 kb on - strand, within Pf1N1B4_65at 68.620 kb on - strand, within Pf1N1B4_65at 68.698 kb on - strandat 68.698 kb on - strandat 68.709 kb on + strandat 68.710 kb on - strandat 68.871 kb on - strand, within Pf1N1B4_66at 69.038 kb on + strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strandat 69.045 kb on - strand, within Pf1N1B4_66at 69.129 kb on + strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strandat 69.150 kb on + strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.271 kb on + strand, within Pf1N1B4_66at 69.330 kb on + strandat 69.330 kb on + strandat 69.395 kb on + strandat 69.432 kb on + strandat 69.432 kb on + strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.473 kb on + strandat 69.474 kb on - strandat 69.474 kb on - strandat 69.476 kb on - strandat 69.476 kb on - strandat 69.639 kb on - strand, within Pf1N1B4_67at 69.729 kb on + strand, within Pf1N1B4_67at 69.798 kb on - strand, within Pf1N1B4_67at 70.129 kb on + strandat 70.160 kb on + strandat 70.161 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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67,838 + +0.9
67,838 + +0.1
67,839 - +0.1
67,839 - -0.9
67,923 + -0.1
67,923 + +0.7
67,923 + -0.1
67,924 - -0.2
67,924 - -0.9
67,924 - +0.7
67,924 - -1.9
67,924 - -0.3
67,924 - +1.3
67,924 - -0.8
67,924 - +2.1
67,927 - -0.1
67,927 - -1.7
68,011 - Pf1N1B4_65 0.10 -0.2
68,011 - Pf1N1B4_65 0.10 -0.9
68,019 + Pf1N1B4_65 0.11 +0.3
68,019 + Pf1N1B4_65 0.11 +0.7
68,019 + Pf1N1B4_65 0.11 +0.2
68,020 - Pf1N1B4_65 0.11 +0.5
68,020 - Pf1N1B4_65 0.11 -0.2
68,020 - Pf1N1B4_65 0.11 +0.1
68,020 - Pf1N1B4_65 0.11 -0.5
68,122 - Pf1N1B4_65 0.24 +0.1
68,122 - Pf1N1B4_65 0.24 -0.5
68,232 - Pf1N1B4_65 0.37 +0.2
68,245 + Pf1N1B4_65 0.39 +1.7
68,246 - -1.7
68,292 + Pf1N1B4_65 0.44 +1.7
68,293 - Pf1N1B4_65 0.44 +0.6
68,293 - Pf1N1B4_65 0.44 +2.1
68,293 - Pf1N1B4_65 0.44 +0.1
68,293 - Pf1N1B4_65 0.44 -0.3
68,293 - Pf1N1B4_65 0.44 -0.2
68,293 - Pf1N1B4_65 0.44 -0.1
68,484 + Pf1N1B4_65 0.67 -2.1
68,485 - Pf1N1B4_65 0.67 -0.3
68,485 - Pf1N1B4_65 0.67 -0.5
68,485 - Pf1N1B4_65 0.67 -0.5
68,619 + Pf1N1B4_65 0.84 +0.4
68,619 + Pf1N1B4_65 0.84 -1.4
68,619 + +1.5
68,620 - Pf1N1B4_65 0.84 -2.1
68,620 - Pf1N1B4_65 0.84 +0.1
68,698 - -0.5
68,698 - -0.3
68,709 + +1.1
68,710 - +0.1
68,871 - Pf1N1B4_66 0.10 +0.1
69,038 + Pf1N1B4_66 0.41 -0.9
69,045 - Pf1N1B4_66 0.42 -1.7
69,045 - Pf1N1B4_66 0.42 -1.7
69,045 - Pf1N1B4_66 0.42 -1.5
69,045 - Pf1N1B4_66 0.42 +0.3
69,045 - -0.9
69,045 - Pf1N1B4_66 0.42 -3.3
69,129 + Pf1N1B4_66 0.58 -0.3
69,130 - Pf1N1B4_66 0.58 -2.0
69,130 - Pf1N1B4_66 0.58 -2.5
69,141 - Pf1N1B4_66 0.60 -1.8
69,141 - Pf1N1B4_66 0.60 -2.4
69,141 - Pf1N1B4_66 0.60 -0.3
69,141 - Pf1N1B4_66 0.60 -1.5
69,141 - -0.9
69,150 + Pf1N1B4_66 0.62 -1.2
69,207 - Pf1N1B4_66 0.73 +0.1
69,207 - Pf1N1B4_66 0.73 -0.9
69,271 + Pf1N1B4_66 0.85 +0.8
69,330 + -0.5
69,330 + -2.2
69,395 + -0.2
69,432 + -1.5
69,432 + +1.1
69,433 - -1.7
69,433 - -1.5
69,433 - -1.7
69,433 - +2.7
69,473 + +0.8
69,474 - -1.2
69,474 - -1.9
69,476 - -3.0
69,476 - -0.9
69,639 - Pf1N1B4_67 0.20 -2.8
69,729 + Pf1N1B4_67 0.33 -1.5
69,798 - Pf1N1B4_67 0.43 -1.6
70,129 + -2.4
70,160 + +1.1
70,161 - -2.1

Or see this region's nucleotide sequence