Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5616 and Pf1N1B4_6052 are separated by 168 nucleotides Pf1N1B4_6052 and Pf1N1B4_5617 are separated by 63 nucleotides Pf1N1B4_5617 and Pf1N1B4_5618 are separated by 33 nucleotides Pf1N1B4_5618 and Pf1N1B4_5619 are separated by 2 nucleotides Pf1N1B4_5619 and Pf1N1B4_5620 are separated by 184 nucleotides
Pf1N1B4_5616 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 41,741 to 43,132
_5616
Pf1N1B4_6052 - tRNA-Gly-GCC, at 43,301 to 43,373
_6052
Pf1N1B4_5617 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 43,437 to 43,997
_5617
Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854
_5618
Pf1N1B4_5619 - BarA-associated response regulator UvrY (= GacA = SirA), at 45,857 to 46,441
_5619
Pf1N1B4_5620 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 46,626 to 47,069
_5620
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1
2 at 43.439 kb on - strand at 44.033 kb on - strand at 44.504 kb on + strand, within Pf1N1B4_5618 at 44.505 kb on - strand at 45.544 kb on + strand, within Pf1N1B4_5618 at 45.544 kb on + strand at 45.545 kb on - strand, within Pf1N1B4_5618 at 45.581 kb on - strand, within Pf1N1B4_5618 at 45.618 kb on + strand, within Pf1N1B4_5618 at 45.718 kb on + strand at 45.761 kb on - strand at 45.761 kb on - strand at 45.955 kb on + strand, within Pf1N1B4_5619 at 45.955 kb on + strand, within Pf1N1B4_5619 at 46.128 kb on + strand at 46.128 kb on + strand, within Pf1N1B4_5619 at 46.129 kb on - strand, within Pf1N1B4_5619 at 46.485 kb on - strand at 46.493 kb on - strand at 46.522 kb on + strand at 46.580 kb on + strand at 46.647 kb on - strand at 46.837 kb on - strand, within Pf1N1B4_5620
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 43,439 - -1.1 44,033 - +0.3 44,504 + Pf1N1B4_5618 0.26 -0.4 44,505 - -0.7 45,544 + Pf1N1B4_5618 0.83 -1.9 45,544 + +1.9 45,545 - Pf1N1B4_5618 0.83 -1.0 45,581 - Pf1N1B4_5618 0.85 -1.4 45,618 + Pf1N1B4_5618 0.87 -0.7 45,718 + -0.1 45,761 - -1.0 45,761 - +0.9 45,955 + Pf1N1B4_5619 0.17 -0.1 45,955 + Pf1N1B4_5619 0.17 -1.1 46,128 + +1.9 46,128 + Pf1N1B4_5619 0.46 +0.1 46,129 - Pf1N1B4_5619 0.46 -1.1 46,485 - +1.6 46,493 - -1.9 46,522 + +0.8 46,580 + +1.5 46,647 - +0.9 46,837 - Pf1N1B4_5620 0.48 -1.7
Or see this region's nucleotide sequence