Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5296

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5293 and Pf1N1B4_5294 are separated by 58 nucleotidesPf1N1B4_5294 and Pf1N1B4_5295 are separated by 109 nucleotidesPf1N1B4_5295 and Pf1N1B4_5296 are separated by 195 nucleotidesPf1N1B4_5296 and Pf1N1B4_5297 are separated by 152 nucleotidesPf1N1B4_5297 and Pf1N1B4_5298 are separated by 20 nucleotidesPf1N1B4_5298 and Pf1N1B4_5299 are separated by 38 nucleotidesPf1N1B4_5299 and Pf1N1B4_5300 are separated by 78 nucleotides Pf1N1B4_5293 - hypothetical protein, at 49,892 to 50,107 _5293 Pf1N1B4_5294 - hypothetical protein, at 50,166 to 50,285 _5294 Pf1N1B4_5295 - hypothetical protein, at 50,395 to 50,679 _5295 Pf1N1B4_5296 - hypothetical protein, at 50,875 to 51,123 _5296 Pf1N1B4_5297 - hypothetical protein, at 51,276 to 51,413 _5297 Pf1N1B4_5298 - hypothetical protein, at 51,434 to 51,667 _5298 Pf1N1B4_5299 - hypothetical protein, at 51,706 to 51,963 _5299 Pf1N1B4_5300 - hypothetical protein, at 52,042 to 54,108 _5300 Position (kb) 50 51 52Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 49.996 kb on + strand, within Pf1N1B4_5293at 49.996 kb on + strand, within Pf1N1B4_5293at 49.996 kb on + strand, within Pf1N1B4_5293at 49.996 kb on + strand, within Pf1N1B4_5293at 49.996 kb on + strand, within Pf1N1B4_5293at 49.997 kb on - strand, within Pf1N1B4_5293at 50.009 kb on - strand, within Pf1N1B4_5293at 50.010 kb on + strand, within Pf1N1B4_5293at 50.011 kb on - strand, within Pf1N1B4_5293at 50.011 kb on - strand, within Pf1N1B4_5293at 50.152 kb on + strandat 50.152 kb on + strandat 50.153 kb on - strandat 50.266 kb on - strand, within Pf1N1B4_5294at 50.266 kb on - strand, within Pf1N1B4_5294at 50.329 kb on + strandat 50.330 kb on - strandat 50.374 kb on + strandat 50.374 kb on + strandat 50.383 kb on + strandat 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strand, within Pf1N1B4_5295at 50.530 kb on + strandat 50.530 kb on + strand, within Pf1N1B4_5295at 50.531 kb on - strand, within Pf1N1B4_5295at 50.531 kb on - strandat 50.531 kb on - strand, within Pf1N1B4_5295at 50.531 kb on - strand, within Pf1N1B4_5295at 50.557 kb on - strand, within Pf1N1B4_5295at 50.557 kb on - strand, within Pf1N1B4_5295at 50.582 kb on + strand, within Pf1N1B4_5295at 50.596 kb on - strand, within Pf1N1B4_5295at 50.658 kb on + strandat 50.658 kb on + strandat 50.699 kb on - strandat 50.791 kb on + strandat 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strandat 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strandat 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.974 kb on + strand, within Pf1N1B4_5296at 50.975 kb on - strand, within Pf1N1B4_5296at 50.975 kb on - strand, within Pf1N1B4_5296at 50.975 kb on - strand, within Pf1N1B4_5296at 51.001 kb on + strand, within Pf1N1B4_5296at 51.001 kb on + strand, within Pf1N1B4_5296at 51.002 kb on - strand, within Pf1N1B4_5296at 51.034 kb on - strand, within Pf1N1B4_5296at 51.166 kb on - strandat 51.166 kb on - strandat 51.373 kb on + strand, within Pf1N1B4_5297at 51.377 kb on + strand, within Pf1N1B4_5297at 51.377 kb on + strand, within Pf1N1B4_5297at 51.452 kb on + strandat 51.452 kb on + strandat 51.452 kb on + strandat 51.530 kb on - strandat 51.537 kb on + strand, within Pf1N1B4_5298at 51.538 kb on - strand, within Pf1N1B4_5298at 51.538 kb on - strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strandat 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.539 kb on + strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.540 kb on - strand, within Pf1N1B4_5298at 51.613 kb on - strand, within Pf1N1B4_5298at 51.685 kb on + strandat 51.685 kb on + strandat 51.686 kb on - strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.695 kb on + strandat 51.696 kb on - strandat 51.696 kb on - strandat 51.699 kb on + strandat 51.699 kb on + strandat 51.699 kb on + strandat 51.699 kb on + strandat 51.700 kb on - strandat 51.700 kb on - strandat 51.821 kb on + strand, within Pf1N1B4_5299at 51.831 kb on + strand, within Pf1N1B4_5299at 51.832 kb on + strandat 51.832 kb on + strand, within Pf1N1B4_5299at 51.832 kb on + strand, within Pf1N1B4_5299at 51.832 kb on + strand, within Pf1N1B4_5299at 51.885 kb on - strandat 51.885 kb on - strand, within Pf1N1B4_5299at 51.931 kb on + strand, within Pf1N1B4_5299at 51.932 kb on - strand, within Pf1N1B4_5299at 52.040 kb on - strandat 52.075 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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49,996 + Pf1N1B4_5293 0.48 -0.6
49,996 + Pf1N1B4_5293 0.48 -0.2
49,996 + Pf1N1B4_5293 0.48 -1.3
49,996 + Pf1N1B4_5293 0.48 +0.6
49,996 + Pf1N1B4_5293 0.48 +0.8
49,997 - Pf1N1B4_5293 0.49 -0.1
50,009 - Pf1N1B4_5293 0.54 +0.4
50,010 + Pf1N1B4_5293 0.55 +0.6
50,011 - Pf1N1B4_5293 0.55 +0.1
50,011 - Pf1N1B4_5293 0.55 +2.2
50,152 + +0.0
50,152 + +0.7
50,153 - +0.4
50,266 - Pf1N1B4_5294 0.83 +0.6
50,266 - Pf1N1B4_5294 0.83 +0.2
50,329 + -1.0
50,330 - -2.0
50,374 + -0.7
50,374 + +1.8
50,383 + -1.8
50,530 + Pf1N1B4_5295 0.47 -1.0
50,530 + Pf1N1B4_5295 0.47 +0.4
50,530 + Pf1N1B4_5295 0.47 -0.1
50,530 + Pf1N1B4_5295 0.47 -0.9
50,530 + Pf1N1B4_5295 0.47 +0.4
50,530 + Pf1N1B4_5295 0.47 +0.2
50,530 + Pf1N1B4_5295 0.47 +0.1
50,530 + Pf1N1B4_5295 0.47 -0.7
50,530 + +1.0
50,530 + Pf1N1B4_5295 0.47 +1.2
50,531 - Pf1N1B4_5295 0.48 -1.4
50,531 - +1.4
50,531 - Pf1N1B4_5295 0.48 +1.7
50,531 - Pf1N1B4_5295 0.48 -0.2
50,557 - Pf1N1B4_5295 0.57 +0.0
50,557 - Pf1N1B4_5295 0.57 -0.6
50,582 + Pf1N1B4_5295 0.66 +0.3
50,596 - Pf1N1B4_5295 0.71 -0.9
50,658 + +0.0
50,658 + -1.2
50,699 - -0.9
50,791 + +0.2
50,974 + Pf1N1B4_5296 0.40 +0.2
50,974 + Pf1N1B4_5296 0.40 -0.2
50,974 + Pf1N1B4_5296 0.40 +0.8
50,974 + -0.2
50,974 + Pf1N1B4_5296 0.40 +1.4
50,974 + -0.6
50,974 + Pf1N1B4_5296 0.40 +1.4
50,974 + Pf1N1B4_5296 0.40 +0.3
50,974 + Pf1N1B4_5296 0.40 +1.4
50,975 - Pf1N1B4_5296 0.40 +1.7
50,975 - Pf1N1B4_5296 0.40 -0.0
50,975 - Pf1N1B4_5296 0.40 +1.4
51,001 + Pf1N1B4_5296 0.51 +0.2
51,001 + Pf1N1B4_5296 0.51 -1.2
51,002 - Pf1N1B4_5296 0.51 +0.7
51,034 - Pf1N1B4_5296 0.64 -0.5
51,166 - -0.4
51,166 - -2.0
51,373 + Pf1N1B4_5297 0.70 +1.1
51,377 + Pf1N1B4_5297 0.73 -1.3
51,377 + Pf1N1B4_5297 0.73 -0.2
51,452 + -1.9
51,452 + -2.0
51,452 + -0.1
51,530 - -0.6
51,537 + Pf1N1B4_5298 0.44 -1.2
51,538 - Pf1N1B4_5298 0.44 +0.2
51,538 - Pf1N1B4_5298 0.44 -0.1
51,539 + Pf1N1B4_5298 0.45 +0.4
51,539 + Pf1N1B4_5298 0.45 -0.2
51,539 + Pf1N1B4_5298 0.45 -0.8
51,539 + +0.4
51,539 + Pf1N1B4_5298 0.45 -0.6
51,539 + Pf1N1B4_5298 0.45 +0.4
51,539 + Pf1N1B4_5298 0.45 -1.2
51,539 + Pf1N1B4_5298 0.45 -1.2
51,539 + Pf1N1B4_5298 0.45 -0.2
51,539 + Pf1N1B4_5298 0.45 -1.2
51,540 - Pf1N1B4_5298 0.45 -0.8
51,540 - Pf1N1B4_5298 0.45 -0.6
51,540 - Pf1N1B4_5298 0.45 -0.2
51,540 - Pf1N1B4_5298 0.45 +4.0
51,540 - Pf1N1B4_5298 0.45 -2.3
51,540 - Pf1N1B4_5298 0.45 -1.2
51,540 - Pf1N1B4_5298 0.45 -0.7
51,540 - Pf1N1B4_5298 0.45 +0.4
51,540 - Pf1N1B4_5298 0.45 +0.7
51,540 - Pf1N1B4_5298 0.45 +1.4
51,540 - Pf1N1B4_5298 0.45 +1.4
51,540 - Pf1N1B4_5298 0.45 -0.7
51,613 - Pf1N1B4_5298 0.76 -0.6
51,685 + -0.7
51,685 + +0.1
51,686 - +0.8
51,695 + +0.4
51,695 + +0.0
51,695 + +1.1
51,695 + +0.4
51,695 + -1.4
51,696 - +2.4
51,696 - -1.2
51,699 + -1.2
51,699 + -2.3
51,699 + -0.2
51,699 + +1.2
51,700 - -0.5
51,700 - +0.2
51,821 + Pf1N1B4_5299 0.45 -0.8
51,831 + Pf1N1B4_5299 0.48 -0.3
51,832 + +1.4
51,832 + Pf1N1B4_5299 0.49 -0.3
51,832 + Pf1N1B4_5299 0.49 +0.2
51,832 + Pf1N1B4_5299 0.49 -0.6
51,885 - +1.4
51,885 - Pf1N1B4_5299 0.69 +0.5
51,931 + Pf1N1B4_5299 0.87 +1.0
51,932 - Pf1N1B4_5299 0.88 -0.2
52,040 - -0.4
52,075 + +0.8

Or see this region's nucleotide sequence