Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5135

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5133 and Pf1N1B4_5134 are separated by 13 nucleotidesPf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotidesPf1N1B4_5135 and Pf1N1B4_5136 are separated by 124 nucleotides Pf1N1B4_5133 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 5,711,996 to 5,713,180 _5133 Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045 _5134 Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685 _5135 Pf1N1B4_5136 - Carboxynorspermidine dehydrogenase, at 5,714,810 to 5,716,045 _5136 Position (kb) 5714 5715Strain fitness (log2 ratio) -1 0 1 2at 5713.718 kb on + strand, within Pf1N1B4_5134at 5713.936 kb on - strand, within Pf1N1B4_5134at 5714.038 kb on - strandat 5714.168 kb on - strand, within Pf1N1B4_5135at 5714.222 kb on - strand, within Pf1N1B4_5135at 5714.222 kb on - strand, within Pf1N1B4_5135at 5714.436 kb on - strand, within Pf1N1B4_5135at 5714.438 kb on + strand, within Pf1N1B4_5135at 5714.439 kb on - strand, within Pf1N1B4_5135at 5714.439 kb on - strand, within Pf1N1B4_5135at 5714.533 kb on + strand, within Pf1N1B4_5135at 5714.616 kb on + strand, within Pf1N1B4_5135at 5714.684 kb on - strandat 5714.771 kb on - strandat 5714.985 kb on + strand, within Pf1N1B4_5136at 5714.986 kb on - strand, within Pf1N1B4_5136at 5714.986 kb on - strand, within Pf1N1B4_5136at 5715.042 kb on + strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.234 kb on - strand, within Pf1N1B4_5136at 5715.252 kb on + strand, within Pf1N1B4_5136at 5715.252 kb on + strand, within Pf1N1B4_5136at 5715.252 kb on + strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strandat 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.340 kb on - strand, within Pf1N1B4_5136at 5715.340 kb on - strand, within Pf1N1B4_5136at 5715.487 kb on - strand, within Pf1N1B4_5136at 5715.536 kb on - strand, within Pf1N1B4_5136at 5715.546 kb on + strand, within Pf1N1B4_5136at 5715.657 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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5,713,718 + Pf1N1B4_5134 0.62 +0.8
5,713,936 - Pf1N1B4_5134 0.87 -0.7
5,714,038 - +0.0
5,714,168 - Pf1N1B4_5135 0.20 +1.2
5,714,222 - Pf1N1B4_5135 0.28 +0.0
5,714,222 - Pf1N1B4_5135 0.28 -0.7
5,714,436 - Pf1N1B4_5135 0.61 -1.2
5,714,438 + Pf1N1B4_5135 0.62 -0.8
5,714,439 - Pf1N1B4_5135 0.62 +0.4
5,714,439 - Pf1N1B4_5135 0.62 +1.1
5,714,533 + Pf1N1B4_5135 0.76 -1.1
5,714,616 + Pf1N1B4_5135 0.89 +2.2
5,714,684 - +0.2
5,714,771 - -0.4
5,714,985 + Pf1N1B4_5136 0.14 +0.2
5,714,986 - Pf1N1B4_5136 0.14 +0.4
5,714,986 - Pf1N1B4_5136 0.14 +0.1
5,715,042 + Pf1N1B4_5136 0.19 +0.8
5,715,043 - Pf1N1B4_5136 0.19 +0.0
5,715,043 - Pf1N1B4_5136 0.19 +0.6
5,715,043 - Pf1N1B4_5136 0.19 +0.2
5,715,043 - Pf1N1B4_5136 0.19 -0.5
5,715,043 - Pf1N1B4_5136 0.19 +0.1
5,715,234 - Pf1N1B4_5136 0.34 +0.3
5,715,252 + Pf1N1B4_5136 0.36 -0.3
5,715,252 + Pf1N1B4_5136 0.36 -0.7
5,715,252 + Pf1N1B4_5136 0.36 -0.7
5,715,253 - Pf1N1B4_5136 0.36 +0.6
5,715,253 - Pf1N1B4_5136 0.36 +0.4
5,715,253 - +0.8
5,715,253 - Pf1N1B4_5136 0.36 -0.3
5,715,253 - Pf1N1B4_5136 0.36 +0.8
5,715,253 - Pf1N1B4_5136 0.36 +0.4
5,715,340 - Pf1N1B4_5136 0.43 +0.4
5,715,340 - Pf1N1B4_5136 0.43 +0.3
5,715,487 - Pf1N1B4_5136 0.55 -0.7
5,715,536 - Pf1N1B4_5136 0.59 -0.1
5,715,546 + Pf1N1B4_5136 0.60 +0.7
5,715,657 + +2.3

Or see this region's nucleotide sequence