Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5133 and Pf1N1B4_5134 are separated by 13 nucleotides Pf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotides Pf1N1B4_5135 and Pf1N1B4_5136 are separated by 124 nucleotides
Pf1N1B4_5133 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 5,711,996 to 5,713,180
_5133
Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045
_5134
Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685
_5135
Pf1N1B4_5136 - Carboxynorspermidine dehydrogenase, at 5,714,810 to 5,716,045
_5136
Position (kb)
5714
5715 Strain fitness (log2 ratio)
-1
0
1
2 at 5713.718 kb on + strand, within Pf1N1B4_5134 at 5713.936 kb on - strand, within Pf1N1B4_5134 at 5714.038 kb on - strand at 5714.168 kb on - strand, within Pf1N1B4_5135 at 5714.222 kb on - strand, within Pf1N1B4_5135 at 5714.222 kb on - strand, within Pf1N1B4_5135 at 5714.436 kb on - strand, within Pf1N1B4_5135 at 5714.438 kb on + strand, within Pf1N1B4_5135 at 5714.439 kb on - strand, within Pf1N1B4_5135 at 5714.439 kb on - strand, within Pf1N1B4_5135 at 5714.533 kb on + strand, within Pf1N1B4_5135 at 5714.616 kb on + strand, within Pf1N1B4_5135 at 5714.684 kb on - strand at 5714.771 kb on - strand at 5714.985 kb on + strand, within Pf1N1B4_5136 at 5714.986 kb on - strand, within Pf1N1B4_5136 at 5714.986 kb on - strand, within Pf1N1B4_5136 at 5715.042 kb on + strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.234 kb on - strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.487 kb on - strand, within Pf1N1B4_5136 at 5715.536 kb on - strand, within Pf1N1B4_5136 at 5715.546 kb on + strand, within Pf1N1B4_5136 at 5715.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 5,713,718 + Pf1N1B4_5134 0.62 +0.8 5,713,936 - Pf1N1B4_5134 0.87 -0.7 5,714,038 - +0.0 5,714,168 - Pf1N1B4_5135 0.20 +1.2 5,714,222 - Pf1N1B4_5135 0.28 +0.0 5,714,222 - Pf1N1B4_5135 0.28 -0.7 5,714,436 - Pf1N1B4_5135 0.61 -1.2 5,714,438 + Pf1N1B4_5135 0.62 -0.8 5,714,439 - Pf1N1B4_5135 0.62 +0.4 5,714,439 - Pf1N1B4_5135 0.62 +1.1 5,714,533 + Pf1N1B4_5135 0.76 -1.1 5,714,616 + Pf1N1B4_5135 0.89 +2.2 5,714,684 - +0.2 5,714,771 - -0.4 5,714,985 + Pf1N1B4_5136 0.14 +0.2 5,714,986 - Pf1N1B4_5136 0.14 +0.4 5,714,986 - Pf1N1B4_5136 0.14 +0.1 5,715,042 + Pf1N1B4_5136 0.19 +0.8 5,715,043 - Pf1N1B4_5136 0.19 +0.0 5,715,043 - Pf1N1B4_5136 0.19 +0.6 5,715,043 - Pf1N1B4_5136 0.19 +0.2 5,715,043 - Pf1N1B4_5136 0.19 -0.5 5,715,043 - Pf1N1B4_5136 0.19 +0.1 5,715,234 - Pf1N1B4_5136 0.34 +0.3 5,715,252 + Pf1N1B4_5136 0.36 -0.3 5,715,252 + Pf1N1B4_5136 0.36 -0.7 5,715,252 + Pf1N1B4_5136 0.36 -0.7 5,715,253 - Pf1N1B4_5136 0.36 +0.6 5,715,253 - Pf1N1B4_5136 0.36 +0.4 5,715,253 - +0.8 5,715,253 - Pf1N1B4_5136 0.36 -0.3 5,715,253 - Pf1N1B4_5136 0.36 +0.8 5,715,253 - Pf1N1B4_5136 0.36 +0.4 5,715,340 - Pf1N1B4_5136 0.43 +0.4 5,715,340 - Pf1N1B4_5136 0.43 +0.3 5,715,487 - Pf1N1B4_5136 0.55 -0.7 5,715,536 - Pf1N1B4_5136 0.59 -0.1 5,715,546 + Pf1N1B4_5136 0.60 +0.7 5,715,657 + +2.3
Or see this region's nucleotide sequence