Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4726 and Pf1N1B4_4727 overlap by 4 nucleotides Pf1N1B4_4727 and Pf1N1B4_4728 are separated by 115 nucleotides Pf1N1B4_4728 and Pf1N1B4_4729 are separated by 77 nucleotides
Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681
_4726
Pf1N1B4_4727 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 5,223,678 to 5,224,499
_4727
Pf1N1B4_4728 - Na+/H+ antiporter NhaB, at 5,224,615 to 5,226,117
_4728
Pf1N1B4_4729 - Hemolysin-type calcium-binding region, at 5,226,195 to 5,227,484
_4729
Position (kb) 5224
5225
5226
5227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5223.910 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.943 kb on - strand, within Pf1N1B4_4727 at 5224.119 kb on + strand at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.617 kb on - strand at 5224.617 kb on - strand at 5224.619 kb on - strand at 5224.626 kb on - strand at 5224.638 kb on - strand at 5224.665 kb on + strand at 5224.666 kb on - strand at 5224.666 kb on - strand at 5224.668 kb on - strand at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.930 kb on - strand, within Pf1N1B4_4728 at 5225.630 kb on + strand, within Pf1N1B4_4728 at 5225.631 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.812 kb on - strand, within Pf1N1B4_4728 at 5225.859 kb on - strand, within Pf1N1B4_4728 at 5226.132 kb on - strand at 5226.135 kb on - strand at 5226.135 kb on - strand at 5226.252 kb on + strand at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.496 kb on - strand at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.539 kb on + strand, within Pf1N1B4_4729 at 5226.852 kb on - strand at 5226.882 kb on + strand, within Pf1N1B4_4729
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 5,223,910 + Pf1N1B4_4727 0.28 +0.8 5,223,942 + Pf1N1B4_4727 0.32 -1.1 5,223,942 + Pf1N1B4_4727 0.32 +0.2 5,223,942 + Pf1N1B4_4727 0.32 +0.3 5,223,942 + Pf1N1B4_4727 0.32 -1.1 5,223,943 - Pf1N1B4_4727 0.32 -0.5 5,224,119 + +0.8 5,224,242 + Pf1N1B4_4727 0.69 +0.8 5,224,242 + Pf1N1B4_4727 0.69 -0.1 5,224,482 + -0.2 5,224,482 + +0.8 5,224,482 + +0.4 5,224,482 + +0.8 5,224,483 - -1.8 5,224,483 - +0.0 5,224,483 - -1.2 5,224,483 - -2.1 5,224,483 - +0.4 5,224,483 - -0.6 5,224,617 - -0.8 5,224,617 - -1.2 5,224,619 - +0.8 5,224,626 - -1.6 5,224,638 - -1.2 5,224,665 + -0.8 5,224,666 - -0.2 5,224,666 - -0.2 5,224,668 - -2.1 5,224,875 - Pf1N1B4_4728 0.17 -0.2 5,224,875 - Pf1N1B4_4728 0.17 -2.2 5,224,875 - Pf1N1B4_4728 0.17 -1.9 5,224,875 - Pf1N1B4_4728 0.17 -1.2 5,224,930 - Pf1N1B4_4728 0.21 -2.4 5,225,630 + Pf1N1B4_4728 0.68 -0.8 5,225,631 - Pf1N1B4_4728 0.68 +0.2 5,225,796 - Pf1N1B4_4728 0.79 -0.8 5,225,796 - Pf1N1B4_4728 0.79 +0.8 5,225,796 - Pf1N1B4_4728 0.79 -1.8 5,225,812 - Pf1N1B4_4728 0.80 -1.5 5,225,859 - Pf1N1B4_4728 0.83 -3.3 5,226,132 - -0.4 5,226,135 - +2.5 5,226,135 - -0.1 5,226,252 + -0.6 5,226,496 - Pf1N1B4_4729 0.23 -1.8 5,226,496 - -0.2 5,226,496 - Pf1N1B4_4729 0.23 -0.8 5,226,496 - Pf1N1B4_4729 0.23 -1.1 5,226,539 + Pf1N1B4_4729 0.27 +0.2 5,226,852 - -0.6 5,226,882 + Pf1N1B4_4729 0.53 +0.3
Or see this region's nucleotide sequence