Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4119

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4118 and Pf1N1B4_4119 are separated by 486 nucleotidesPf1N1B4_4119 and Pf1N1B4_4120 are separated by 67 nucleotidesPf1N1B4_4120 and Pf1N1B4_4121 are separated by 101 nucleotides Pf1N1B4_4118 - Cyn operon transcriptional activator, at 4,560,664 to 4,561,593 _4118 Pf1N1B4_4119 - dioxygenase, at 4,562,080 to 4,562,391 _4119 Pf1N1B4_4120 - hypothetical protein, at 4,562,459 to 4,562,647 _4120 Pf1N1B4_4121 - putative lipoprotein, at 4,562,749 to 4,563,291 _4121 Position (kb) 4562 4563Strain fitness (log2 ratio) -2 -1 0 1at 4561.151 kb on - strand, within Pf1N1B4_4118at 4561.151 kb on - strand, within Pf1N1B4_4118at 4561.159 kb on + strandat 4561.159 kb on + strand, within Pf1N1B4_4118at 4561.165 kb on + strand, within Pf1N1B4_4118at 4561.447 kb on + strand, within Pf1N1B4_4118at 4561.592 kb on - strandat 4561.684 kb on - strandat 4561.742 kb on + strandat 4561.837 kb on + strandat 4561.838 kb on - strandat 4561.838 kb on - strandat 4561.838 kb on - strandat 4561.892 kb on + strandat 4561.892 kb on + strandat 4561.892 kb on + strandat 4561.892 kb on + strandat 4561.893 kb on - strandat 4561.893 kb on - strandat 4561.922 kb on + strandat 4561.975 kb on + strandat 4562.079 kb on + strandat 4562.084 kb on + strandat 4562.146 kb on + strand, within Pf1N1B4_4119at 4562.147 kb on - strand, within Pf1N1B4_4119at 4562.185 kb on + strand, within Pf1N1B4_4119at 4562.186 kb on - strand, within Pf1N1B4_4119at 4562.186 kb on - strand, within Pf1N1B4_4119at 4562.279 kb on + strand, within Pf1N1B4_4119at 4562.279 kb on + strand, within Pf1N1B4_4119at 4562.417 kb on + strandat 4562.587 kb on + strand, within Pf1N1B4_4120at 4562.588 kb on - strand, within Pf1N1B4_4120at 4562.646 kb on - strandat 4562.646 kb on - strandat 4562.713 kb on + strandat 4562.714 kb on - strandat 4562.714 kb on - strandat 4562.829 kb on - strand, within Pf1N1B4_4121at 4562.888 kb on - strand, within Pf1N1B4_4121at 4562.888 kb on - strand, within Pf1N1B4_4121at 4562.932 kb on + strandat 4562.932 kb on + strandat 4562.933 kb on - strand, within Pf1N1B4_4121at 4562.933 kb on - strandat 4562.933 kb on - strand, within Pf1N1B4_4121at 4562.936 kb on + strand, within Pf1N1B4_4121at 4562.936 kb on + strand, within Pf1N1B4_4121at 4562.936 kb on + strand, within Pf1N1B4_4121at 4562.937 kb on - strand, within Pf1N1B4_4121at 4562.937 kb on - strand, within Pf1N1B4_4121at 4562.937 kb on - strand, within Pf1N1B4_4121at 4562.937 kb on - strandat 4562.937 kb on - strand, within Pf1N1B4_4121at 4562.937 kb on - strand, within Pf1N1B4_4121at 4562.973 kb on - strand, within Pf1N1B4_4121at 4562.979 kb on - strand, within Pf1N1B4_4121at 4563.026 kb on + strand, within Pf1N1B4_4121at 4563.027 kb on - strand, within Pf1N1B4_4121at 4563.059 kb on - strand, within Pf1N1B4_4121at 4563.059 kb on - strand, within Pf1N1B4_4121at 4563.072 kb on - strand, within Pf1N1B4_4121at 4563.170 kb on + strand, within Pf1N1B4_4121at 4563.306 kb on + strandat 4563.310 kb on - strandat 4563.310 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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4,561,151 - Pf1N1B4_4118 0.52 -0.1
4,561,151 - Pf1N1B4_4118 0.52 +0.9
4,561,159 + +0.9
4,561,159 + Pf1N1B4_4118 0.53 +0.9
4,561,165 + Pf1N1B4_4118 0.54 +0.2
4,561,447 + Pf1N1B4_4118 0.84 +1.2
4,561,592 - -0.3
4,561,684 - -0.6
4,561,742 + -0.1
4,561,837 + -1.6
4,561,838 - -0.1
4,561,838 - +0.9
4,561,838 - +0.7
4,561,892 + +0.2
4,561,892 + -0.8
4,561,892 + +1.3
4,561,892 + -0.5
4,561,893 - -1.2
4,561,893 - +0.7
4,561,922 + +1.5
4,561,975 + -1.2
4,562,079 + +1.2
4,562,084 + -1.9
4,562,146 + Pf1N1B4_4119 0.21 +0.6
4,562,147 - Pf1N1B4_4119 0.21 -0.9
4,562,185 + Pf1N1B4_4119 0.34 -1.4
4,562,186 - Pf1N1B4_4119 0.34 -0.1
4,562,186 - Pf1N1B4_4119 0.34 -1.1
4,562,279 + Pf1N1B4_4119 0.64 -2.1
4,562,279 + Pf1N1B4_4119 0.64 -2.2
4,562,417 + -0.6
4,562,587 + Pf1N1B4_4120 0.68 -1.1
4,562,588 - Pf1N1B4_4120 0.68 +1.1
4,562,646 - -0.5
4,562,646 - +0.2
4,562,713 + +0.6
4,562,714 - +0.9
4,562,714 - +0.4
4,562,829 - Pf1N1B4_4121 0.15 -1.5
4,562,888 - Pf1N1B4_4121 0.26 -0.3
4,562,888 - Pf1N1B4_4121 0.26 -0.4
4,562,932 + +1.5
4,562,932 + +0.2
4,562,933 - Pf1N1B4_4121 0.34 +0.9
4,562,933 - +0.9
4,562,933 - Pf1N1B4_4121 0.34 +0.4
4,562,936 + Pf1N1B4_4121 0.34 -0.4
4,562,936 + Pf1N1B4_4121 0.34 -1.6
4,562,936 + Pf1N1B4_4121 0.34 +0.2
4,562,937 - Pf1N1B4_4121 0.35 +0.6
4,562,937 - Pf1N1B4_4121 0.35 -0.1
4,562,937 - Pf1N1B4_4121 0.35 -1.6
4,562,937 - -0.1
4,562,937 - Pf1N1B4_4121 0.35 +0.1
4,562,937 - Pf1N1B4_4121 0.35 -2.4
4,562,973 - Pf1N1B4_4121 0.41 +0.7
4,562,979 - Pf1N1B4_4121 0.42 +0.9
4,563,026 + Pf1N1B4_4121 0.51 -1.0
4,563,027 - Pf1N1B4_4121 0.51 +0.7
4,563,059 - Pf1N1B4_4121 0.57 +0.2
4,563,059 - Pf1N1B4_4121 0.57 -0.5
4,563,072 - Pf1N1B4_4121 0.59 -1.1
4,563,170 + Pf1N1B4_4121 0.78 +0.2
4,563,306 + -2.1
4,563,310 - +0.6
4,563,310 - +0.9

Or see this region's nucleotide sequence