Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4100

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4098 and Pf1N1B4_4099 are separated by 82 nucleotidesPf1N1B4_4099 and Pf1N1B4_4100 overlap by 31 nucleotidesPf1N1B4_4100 and Pf1N1B4_4101 are separated by 317 nucleotides Pf1N1B4_4098 - Putative nucleoside transporter yegT, at 4,547,271 to 4,548,473 _4098 Pf1N1B4_4099 - hypothetical protein, at 4,548,556 to 4,548,681 _4099 Pf1N1B4_4100 - probable transmembrane protein, at 4,548,651 to 4,549,466 _4100 Pf1N1B4_4101 - Transcriptional regulator, AraC family, at 4,549,784 to 4,550,575 _4101 Position (kb) 4548 4549 4550Strain fitness (log2 ratio) -2 -1 0 1 2at 4547.839 kb on + strand, within Pf1N1B4_4098at 4547.889 kb on + strand, within Pf1N1B4_4098at 4547.913 kb on - strandat 4548.192 kb on + strand, within Pf1N1B4_4098at 4548.222 kb on + strand, within Pf1N1B4_4098at 4548.222 kb on + strand, within Pf1N1B4_4098at 4548.223 kb on - strand, within Pf1N1B4_4098at 4548.289 kb on - strand, within Pf1N1B4_4098at 4548.342 kb on + strand, within Pf1N1B4_4098at 4548.342 kb on + strand, within Pf1N1B4_4098at 4548.342 kb on + strand, within Pf1N1B4_4098at 4548.612 kb on - strand, within Pf1N1B4_4099at 4548.612 kb on - strand, within Pf1N1B4_4099at 4548.614 kb on + strand, within Pf1N1B4_4099at 4548.614 kb on + strandat 4548.615 kb on - strand, within Pf1N1B4_4099at 4548.712 kb on + strandat 4548.712 kb on + strandat 4548.712 kb on + strandat 4548.713 kb on - strandat 4548.713 kb on - strandat 4548.879 kb on - strand, within Pf1N1B4_4100at 4548.907 kb on + strand, within Pf1N1B4_4100at 4548.908 kb on - strand, within Pf1N1B4_4100at 4548.908 kb on - strand, within Pf1N1B4_4100at 4548.908 kb on - strand, within Pf1N1B4_4100at 4549.161 kb on - strand, within Pf1N1B4_4100at 4549.482 kb on - strandat 4549.482 kb on - strandat 4549.482 kb on - strandat 4549.569 kb on - strandat 4549.877 kb on - strand, within Pf1N1B4_4101at 4549.881 kb on - strand, within Pf1N1B4_4101at 4549.909 kb on - strand, within Pf1N1B4_4101at 4550.033 kb on + strand, within Pf1N1B4_4101at 4550.033 kb on + strand, within Pf1N1B4_4101at 4550.034 kb on - strand, within Pf1N1B4_4101at 4550.061 kb on + strand, within Pf1N1B4_4101at 4550.062 kb on - strand, within Pf1N1B4_4101at 4550.099 kb on - strandat 4550.099 kb on - strand, within Pf1N1B4_4101at 4550.099 kb on - strand, within Pf1N1B4_4101at 4550.099 kb on - strand, within Pf1N1B4_4101at 4550.099 kb on - strand, within Pf1N1B4_4101at 4550.099 kb on - strandat 4550.121 kb on - strand, within Pf1N1B4_4101at 4550.381 kb on + strand, within Pf1N1B4_4101at 4550.382 kb on - strand, within Pf1N1B4_4101at 4550.387 kb on - strand, within Pf1N1B4_4101at 4550.402 kb on + strand, within Pf1N1B4_4101at 4550.402 kb on + strand, within Pf1N1B4_4101at 4550.402 kb on + strand, within Pf1N1B4_4101at 4550.403 kb on - strand, within Pf1N1B4_4101at 4550.403 kb on - strand, within Pf1N1B4_4101

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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4,547,839 + Pf1N1B4_4098 0.47 +1.4
4,547,889 + Pf1N1B4_4098 0.51 -1.6
4,547,913 - -0.0
4,548,192 + Pf1N1B4_4098 0.77 +0.1
4,548,222 + Pf1N1B4_4098 0.79 -2.0
4,548,222 + Pf1N1B4_4098 0.79 -0.0
4,548,223 - Pf1N1B4_4098 0.79 -0.6
4,548,289 - Pf1N1B4_4098 0.85 +0.1
4,548,342 + Pf1N1B4_4098 0.89 -0.4
4,548,342 + Pf1N1B4_4098 0.89 -0.6
4,548,342 + Pf1N1B4_4098 0.89 -0.6
4,548,612 - Pf1N1B4_4099 0.44 +1.0
4,548,612 - Pf1N1B4_4099 0.44 -0.6
4,548,614 + Pf1N1B4_4099 0.46 +0.3
4,548,614 + +0.4
4,548,615 - Pf1N1B4_4099 0.47 -0.6
4,548,712 + -0.6
4,548,712 + +0.1
4,548,712 + +1.3
4,548,713 - +1.0
4,548,713 - +0.1
4,548,879 - Pf1N1B4_4100 0.28 +2.4
4,548,907 + Pf1N1B4_4100 0.31 -1.4
4,548,908 - Pf1N1B4_4100 0.31 +0.4
4,548,908 - Pf1N1B4_4100 0.31 -2.6
4,548,908 - Pf1N1B4_4100 0.31 +0.5
4,549,161 - Pf1N1B4_4100 0.62 -2.2
4,549,482 - -1.0
4,549,482 - +0.5
4,549,482 - -0.2
4,549,569 - -0.0
4,549,877 - Pf1N1B4_4101 0.12 -0.4
4,549,881 - Pf1N1B4_4101 0.12 +0.7
4,549,909 - Pf1N1B4_4101 0.16 +1.0
4,550,033 + Pf1N1B4_4101 0.31 +0.4
4,550,033 + Pf1N1B4_4101 0.31 -0.0
4,550,034 - Pf1N1B4_4101 0.32 -2.0
4,550,061 + Pf1N1B4_4101 0.35 -0.6
4,550,062 - Pf1N1B4_4101 0.35 -0.9
4,550,099 - +0.4
4,550,099 - Pf1N1B4_4101 0.40 +1.0
4,550,099 - Pf1N1B4_4101 0.40 -0.4
4,550,099 - Pf1N1B4_4101 0.40 +0.6
4,550,099 - Pf1N1B4_4101 0.40 -1.8
4,550,099 - -0.6
4,550,121 - Pf1N1B4_4101 0.43 -0.4
4,550,381 + Pf1N1B4_4101 0.75 +1.0
4,550,382 - Pf1N1B4_4101 0.76 -0.4
4,550,387 - Pf1N1B4_4101 0.76 -1.7
4,550,402 + Pf1N1B4_4101 0.78 -0.6
4,550,402 + Pf1N1B4_4101 0.78 -0.3
4,550,402 + Pf1N1B4_4101 0.78 +0.5
4,550,403 - Pf1N1B4_4101 0.78 -0.0
4,550,403 - Pf1N1B4_4101 0.78 -0.6

Or see this region's nucleotide sequence