Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3644 and Pf1N1B4_3645 are separated by 11 nucleotides Pf1N1B4_3645 and Pf1N1B4_3646 overlap by 4 nucleotides Pf1N1B4_3646 and Pf1N1B4_3647 overlap by 8 nucleotides Pf1N1B4_3647 and Pf1N1B4_3648 are separated by 474 nucleotides
Pf1N1B4_3644 - Cytochrome c heme lyase subunit CcmF, at 4,049,334 to 4,051,322
_3644
Pf1N1B4_3645 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 4,051,334 to 4,051,855
_3645
Pf1N1B4_3646 - Cytochrome c heme lyase subunit CcmL, at 4,051,852 to 4,052,325
_3646
Pf1N1B4_3647 - Cytochrome c heme lyase subunit CcmH, at 4,052,318 to 4,053,520
_3647
Pf1N1B4_3648 - hypothetical protein, at 4,053,995 to 4,054,498
_3648
Position (kb)
4052
4053
4054 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4051.575 kb on + strand, within Pf1N1B4_3645 at 4051.823 kb on + strand at 4051.823 kb on + strand at 4052.308 kb on - strand at 4052.646 kb on - strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.777 kb on + strand, within Pf1N1B4_3647 at 4052.778 kb on - strand, within Pf1N1B4_3647 at 4052.778 kb on - strand, within Pf1N1B4_3647 at 4052.778 kb on - strand, within Pf1N1B4_3647 at 4052.778 kb on - strand, within Pf1N1B4_3647 at 4052.778 kb on - strand, within Pf1N1B4_3647 at 4052.778 kb on - strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.795 kb on + strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.796 kb on - strand, within Pf1N1B4_3647 at 4052.847 kb on + strand, within Pf1N1B4_3647 at 4052.847 kb on + strand, within Pf1N1B4_3647 at 4052.847 kb on + strand, within Pf1N1B4_3647 at 4052.847 kb on + strand, within Pf1N1B4_3647 at 4052.848 kb on - strand at 4052.848 kb on - strand, within Pf1N1B4_3647 at 4052.848 kb on - strand, within Pf1N1B4_3647 at 4052.900 kb on + strand, within Pf1N1B4_3647 at 4052.900 kb on + strand, within Pf1N1B4_3647 at 4052.900 kb on + strand at 4052.900 kb on + strand at 4052.900 kb on + strand, within Pf1N1B4_3647 at 4052.901 kb on - strand at 4052.901 kb on - strand, within Pf1N1B4_3647 at 4052.901 kb on - strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.082 kb on + strand, within Pf1N1B4_3647 at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.083 kb on - strand at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.083 kb on - strand, within Pf1N1B4_3647 at 4053.173 kb on - strand, within Pf1N1B4_3647 at 4053.518 kb on + strand at 4053.518 kb on + strand at 4053.518 kb on + strand at 4053.519 kb on - strand at 4053.568 kb on - strand at 4053.568 kb on - strand at 4053.756 kb on - strand at 4053.783 kb on + strand at 4053.783 kb on + strand at 4053.784 kb on - strand at 4053.828 kb on + strand at 4053.828 kb on + strand at 4053.829 kb on - strand at 4053.829 kb on - strand at 4053.829 kb on - strand at 4053.829 kb on - strand at 4053.829 kb on - strand at 4054.068 kb on + strand at 4054.068 kb on + strand, within Pf1N1B4_3648 at 4054.068 kb on + strand, within Pf1N1B4_3648 at 4054.097 kb on + strand, within Pf1N1B4_3648 at 4054.097 kb on + strand, within Pf1N1B4_3648 at 4054.097 kb on + strand, within Pf1N1B4_3648 at 4054.098 kb on - strand, within Pf1N1B4_3648 at 4054.098 kb on - strand, within Pf1N1B4_3648 at 4054.098 kb on - strand, within Pf1N1B4_3648 at 4054.098 kb on - strand, within Pf1N1B4_3648 at 4054.098 kb on - strand, within Pf1N1B4_3648 at 4054.098 kb on - strand, within Pf1N1B4_3648 at 4054.265 kb on + strand, within Pf1N1B4_3648 at 4054.333 kb on - strand, within Pf1N1B4_3648 at 4054.448 kb on + strand at 4054.448 kb on + strand at 4054.448 kb on + strand at 4054.448 kb on + strand at 4054.449 kb on - strand at 4054.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 4,051,575 + Pf1N1B4_3645 0.46 -0.5 4,051,823 + -0.7 4,051,823 + -1.2 4,052,308 - +0.3 4,052,646 - Pf1N1B4_3647 0.27 -1.5 4,052,777 + Pf1N1B4_3647 0.38 +0.1 4,052,777 + Pf1N1B4_3647 0.38 -1.5 4,052,777 + Pf1N1B4_3647 0.38 -0.8 4,052,777 + Pf1N1B4_3647 0.38 -1.4 4,052,777 + Pf1N1B4_3647 0.38 +0.4 4,052,777 + Pf1N1B4_3647 0.38 -0.2 4,052,777 + Pf1N1B4_3647 0.38 -0.5 4,052,777 + Pf1N1B4_3647 0.38 -2.5 4,052,777 + Pf1N1B4_3647 0.38 -0.5 4,052,777 + Pf1N1B4_3647 0.38 +2.7 4,052,777 + Pf1N1B4_3647 0.38 -0.5 4,052,777 + Pf1N1B4_3647 0.38 -0.5 4,052,777 + Pf1N1B4_3647 0.38 -1.1 4,052,777 + Pf1N1B4_3647 0.38 -0.8 4,052,777 + Pf1N1B4_3647 0.38 -0.8 4,052,778 - Pf1N1B4_3647 0.38 -1.2 4,052,778 - Pf1N1B4_3647 0.38 -0.5 4,052,778 - Pf1N1B4_3647 0.38 -0.1 4,052,778 - Pf1N1B4_3647 0.38 +0.1 4,052,778 - Pf1N1B4_3647 0.38 -2.3 4,052,778 - Pf1N1B4_3647 0.38 -1.2 4,052,795 + Pf1N1B4_3647 0.40 -1.2 4,052,795 + Pf1N1B4_3647 0.40 -0.1 4,052,795 + Pf1N1B4_3647 0.40 -0.1 4,052,795 + Pf1N1B4_3647 0.40 -1.6 4,052,795 + Pf1N1B4_3647 0.40 -1.5 4,052,795 + Pf1N1B4_3647 0.40 +0.1 4,052,795 + Pf1N1B4_3647 0.40 -0.5 4,052,795 + Pf1N1B4_3647 0.40 -0.0 4,052,795 + Pf1N1B4_3647 0.40 -1.1 4,052,795 + Pf1N1B4_3647 0.40 -0.5 4,052,795 + Pf1N1B4_3647 0.40 +0.6 4,052,795 + Pf1N1B4_3647 0.40 -1.1 4,052,795 + Pf1N1B4_3647 0.40 +0.1 4,052,795 + Pf1N1B4_3647 0.40 +2.3 4,052,795 + Pf1N1B4_3647 0.40 -1.5 4,052,795 + Pf1N1B4_3647 0.40 -1.9 4,052,795 + Pf1N1B4_3647 0.40 +0.6 4,052,795 + Pf1N1B4_3647 0.40 +0.3 4,052,795 + Pf1N1B4_3647 0.40 -1.2 4,052,795 + Pf1N1B4_3647 0.40 -1.8 4,052,796 - Pf1N1B4_3647 0.40 -1.3 4,052,796 - Pf1N1B4_3647 0.40 +0.5 4,052,796 - Pf1N1B4_3647 0.40 -1.3 4,052,796 - Pf1N1B4_3647 0.40 -1.5 4,052,796 - +0.1 4,052,796 - Pf1N1B4_3647 0.40 -2.1 4,052,796 - -1.7 4,052,796 - Pf1N1B4_3647 0.40 -2.1 4,052,796 - Pf1N1B4_3647 0.40 -0.5 4,052,796 - Pf1N1B4_3647 0.40 -0.5 4,052,796 - Pf1N1B4_3647 0.40 +0.3 4,052,796 - Pf1N1B4_3647 0.40 -1.2 4,052,796 - Pf1N1B4_3647 0.40 -1.5 4,052,796 - Pf1N1B4_3647 0.40 +0.4 4,052,796 - Pf1N1B4_3647 0.40 -0.5 4,052,796 - Pf1N1B4_3647 0.40 -0.9 4,052,847 + Pf1N1B4_3647 0.44 +0.1 4,052,847 + Pf1N1B4_3647 0.44 -0.8 4,052,847 + Pf1N1B4_3647 0.44 -2.8 4,052,847 + Pf1N1B4_3647 0.44 +0.4 4,052,848 - +0.1 4,052,848 - Pf1N1B4_3647 0.44 -0.9 4,052,848 - Pf1N1B4_3647 0.44 +0.1 4,052,900 + Pf1N1B4_3647 0.48 -2.2 4,052,900 + Pf1N1B4_3647 0.48 +1.1 4,052,900 + -1.2 4,052,900 + -0.9 4,052,900 + Pf1N1B4_3647 0.48 -1.2 4,052,901 - -0.9 4,052,901 - Pf1N1B4_3647 0.48 -2.5 4,052,901 - Pf1N1B4_3647 0.48 -3.0 4,053,082 + Pf1N1B4_3647 0.64 -0.9 4,053,082 + Pf1N1B4_3647 0.64 -1.9 4,053,082 + Pf1N1B4_3647 0.64 +0.1 4,053,082 + Pf1N1B4_3647 0.64 -0.9 4,053,082 + Pf1N1B4_3647 0.64 -0.5 4,053,082 + Pf1N1B4_3647 0.64 +0.5 4,053,082 + Pf1N1B4_3647 0.64 -0.5 4,053,082 + Pf1N1B4_3647 0.64 -1.9 4,053,082 + Pf1N1B4_3647 0.64 -0.1 4,053,083 - Pf1N1B4_3647 0.64 -1.0 4,053,083 - Pf1N1B4_3647 0.64 +2.4 4,053,083 - Pf1N1B4_3647 0.64 -0.6 4,053,083 - Pf1N1B4_3647 0.64 -1.1 4,053,083 - +0.5 4,053,083 - Pf1N1B4_3647 0.64 -0.2 4,053,083 - Pf1N1B4_3647 0.64 -1.2 4,053,083 - Pf1N1B4_3647 0.64 -1.9 4,053,173 - Pf1N1B4_3647 0.71 -1.0 4,053,518 + +2.1 4,053,518 + +0.6 4,053,518 + +0.7 4,053,519 - -1.1 4,053,568 - +0.1 4,053,568 - -0.2 4,053,756 - -0.6 4,053,783 + -0.3 4,053,783 + -0.4 4,053,784 - -0.9 4,053,828 + -0.3 4,053,828 + +0.4 4,053,829 - -0.6 4,053,829 - -1.2 4,053,829 - -0.5 4,053,829 - -0.1 4,053,829 - +1.1 4,054,068 + +1.7 4,054,068 + Pf1N1B4_3648 0.14 -0.2 4,054,068 + Pf1N1B4_3648 0.14 -0.9 4,054,097 + Pf1N1B4_3648 0.20 +0.7 4,054,097 + Pf1N1B4_3648 0.20 +0.7 4,054,097 + Pf1N1B4_3648 0.20 -0.5 4,054,098 - Pf1N1B4_3648 0.20 -0.1 4,054,098 - Pf1N1B4_3648 0.20 -0.2 4,054,098 - Pf1N1B4_3648 0.20 -1.2 4,054,098 - Pf1N1B4_3648 0.20 +0.8 4,054,098 - Pf1N1B4_3648 0.20 +0.8 4,054,098 - Pf1N1B4_3648 0.20 +1.1 4,054,265 + Pf1N1B4_3648 0.54 +0.6 4,054,333 - Pf1N1B4_3648 0.67 -1.0 4,054,448 + +0.4 4,054,448 + +0.7 4,054,448 + -0.5 4,054,448 + -2.8 4,054,449 - +0.1 4,054,449 - +0.7
Or see this region's nucleotide sequence