Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3610 and Pf1N1B4_3611 are separated by 31 nucleotides Pf1N1B4_3611 and Pf1N1B4_3612 are separated by 83 nucleotides Pf1N1B4_3612 and Pf1N1B4_3613 are separated by 47 nucleotides Pf1N1B4_3613 and Pf1N1B4_3614 are separated by 60 nucleotides Pf1N1B4_3614 and Pf1N1B4_3615 are separated by 9 nucleotides
Pf1N1B4_3610 - Serine phosphatase RsbU, regulator of sigma subunit, at 4,020,417 to 4,022,120
_3610
Pf1N1B4_3611 - Hpt domain protein, at 4,022,152 to 4,022,520
_3611
Pf1N1B4_3612 - Flagellar hook-length control protein FliK, at 4,022,604 to 4,023,965
_3612
Pf1N1B4_3613 - hypothetical protein, at 4,024,013 to 4,024,129
_3613
Pf1N1B4_3614 - Flagellar biosynthesis protein FliL, at 4,024,190 to 4,024,693
_3614
Pf1N1B4_3615 - Flagellar motor switch protein FliM, at 4,024,703 to 4,025,671
_3615
Position (kb)
4022
4023
4024 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4021.689 kb on + strand at 4021.689 kb on + strand, within Pf1N1B4_3610 at 4021.746 kb on + strand, within Pf1N1B4_3610 at 4021.746 kb on + strand, within Pf1N1B4_3610 at 4021.747 kb on - strand, within Pf1N1B4_3610 at 4021.845 kb on + strand, within Pf1N1B4_3610 at 4021.887 kb on + strand, within Pf1N1B4_3610 at 4021.887 kb on + strand, within Pf1N1B4_3610 at 4021.888 kb on - strand, within Pf1N1B4_3610 at 4021.888 kb on - strand at 4022.313 kb on + strand at 4022.325 kb on + strand, within Pf1N1B4_3611 at 4022.325 kb on + strand, within Pf1N1B4_3611 at 4022.464 kb on + strand, within Pf1N1B4_3611 at 4022.518 kb on + strand at 4022.561 kb on + strand at 4022.571 kb on + strand at 4022.572 kb on - strand at 4022.575 kb on + strand at 4022.575 kb on + strand at 4022.575 kb on + strand at 4022.727 kb on + strand at 4022.727 kb on + strand at 4022.728 kb on - strand at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.954 kb on + strand at 4023.954 kb on + strand at 4023.958 kb on - strand at 4023.958 kb on - strand at 4023.958 kb on - strand at 4024.015 kb on - strand at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.129 kb on + strand at 4024.184 kb on - strand at 4024.184 kb on - strand at 4024.195 kb on + strand at 4024.196 kb on - strand at 4024.418 kb on + strand, within Pf1N1B4_3614 at 4024.418 kb on + strand, within Pf1N1B4_3614 at 4024.418 kb on + strand, within Pf1N1B4_3614 at 4024.419 kb on - strand, within Pf1N1B4_3614 at 4024.419 kb on - strand, within Pf1N1B4_3614 at 4024.419 kb on - strand, within Pf1N1B4_3614 at 4024.442 kb on + strand, within Pf1N1B4_3614 at 4024.443 kb on - strand, within Pf1N1B4_3614 at 4024.443 kb on - strand, within Pf1N1B4_3614 at 4024.443 kb on - strand, within Pf1N1B4_3614 at 4024.691 kb on + strand at 4024.692 kb on - strand at 4024.814 kb on + strand, within Pf1N1B4_3615 at 4024.815 kb on - strand, within Pf1N1B4_3615 at 4024.815 kb on - strand, within Pf1N1B4_3615 at 4024.815 kb on - strand, within Pf1N1B4_3615
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 4,021,689 + +1.6 4,021,689 + Pf1N1B4_3610 0.75 -0.2 4,021,746 + Pf1N1B4_3610 0.78 +0.9 4,021,746 + Pf1N1B4_3610 0.78 +0.4 4,021,747 - Pf1N1B4_3610 0.78 +0.8 4,021,845 + Pf1N1B4_3610 0.84 +1.0 4,021,887 + Pf1N1B4_3610 0.86 +0.7 4,021,887 + Pf1N1B4_3610 0.86 -0.7 4,021,888 - Pf1N1B4_3610 0.86 -0.8 4,021,888 - +1.2 4,022,313 + +1.1 4,022,325 + Pf1N1B4_3611 0.47 -0.9 4,022,325 + Pf1N1B4_3611 0.47 +0.9 4,022,464 + Pf1N1B4_3611 0.85 -2.0 4,022,518 + +0.6 4,022,561 + -0.4 4,022,571 + -0.3 4,022,572 - -2.0 4,022,575 + +0.7 4,022,575 + +0.4 4,022,575 + -0.4 4,022,727 + +0.3 4,022,727 + +0.3 4,022,728 - +1.7 4,023,564 + Pf1N1B4_3612 0.70 -1.7 4,023,564 + Pf1N1B4_3612 0.70 -1.7 4,023,564 + Pf1N1B4_3612 0.70 +1.0 4,023,564 + Pf1N1B4_3612 0.70 -0.9 4,023,564 + Pf1N1B4_3612 0.70 +1.6 4,023,564 + Pf1N1B4_3612 0.70 -1.2 4,023,564 + -0.9 4,023,564 + Pf1N1B4_3612 0.70 -0.2 4,023,565 - Pf1N1B4_3612 0.71 -1.7 4,023,565 - Pf1N1B4_3612 0.71 -0.9 4,023,565 - Pf1N1B4_3612 0.71 -0.9 4,023,565 - Pf1N1B4_3612 0.71 -0.8 4,023,565 - Pf1N1B4_3612 0.71 +1.1 4,023,954 + -0.1 4,023,954 + +1.9 4,023,958 - +0.9 4,023,958 - -1.7 4,023,958 - -0.3 4,024,015 - +1.1 4,024,029 - Pf1N1B4_3613 0.14 -0.2 4,024,029 - Pf1N1B4_3613 0.14 -0.4 4,024,029 - -0.4 4,024,029 - Pf1N1B4_3613 0.14 -0.7 4,024,029 - Pf1N1B4_3613 0.14 +1.7 4,024,029 - Pf1N1B4_3613 0.14 -0.2 4,024,129 + +1.9 4,024,184 - -1.0 4,024,184 - +0.8 4,024,195 + +1.1 4,024,196 - +0.3 4,024,418 + Pf1N1B4_3614 0.45 -0.6 4,024,418 + Pf1N1B4_3614 0.45 +0.7 4,024,418 + Pf1N1B4_3614 0.45 -0.9 4,024,419 - Pf1N1B4_3614 0.45 -1.2 4,024,419 - Pf1N1B4_3614 0.45 -0.1 4,024,419 - Pf1N1B4_3614 0.45 -1.3 4,024,442 + Pf1N1B4_3614 0.50 +0.1 4,024,443 - Pf1N1B4_3614 0.50 +0.4 4,024,443 - Pf1N1B4_3614 0.50 -0.6 4,024,443 - Pf1N1B4_3614 0.50 +0.0 4,024,691 + +3.0 4,024,692 - -0.2 4,024,814 + Pf1N1B4_3615 0.11 -0.0 4,024,815 - Pf1N1B4_3615 0.12 -1.7 4,024,815 - Pf1N1B4_3615 0.12 -1.0 4,024,815 - Pf1N1B4_3615 0.12 -2.2
Or see this region's nucleotide sequence