Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3612

Experiment: copper (II) chloride 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3610 and Pf1N1B4_3611 are separated by 31 nucleotidesPf1N1B4_3611 and Pf1N1B4_3612 are separated by 83 nucleotidesPf1N1B4_3612 and Pf1N1B4_3613 are separated by 47 nucleotidesPf1N1B4_3613 and Pf1N1B4_3614 are separated by 60 nucleotidesPf1N1B4_3614 and Pf1N1B4_3615 are separated by 9 nucleotides Pf1N1B4_3610 - Serine phosphatase RsbU, regulator of sigma subunit, at 4,020,417 to 4,022,120 _3610 Pf1N1B4_3611 - Hpt domain protein, at 4,022,152 to 4,022,520 _3611 Pf1N1B4_3612 - Flagellar hook-length control protein FliK, at 4,022,604 to 4,023,965 _3612 Pf1N1B4_3613 - hypothetical protein, at 4,024,013 to 4,024,129 _3613 Pf1N1B4_3614 - Flagellar biosynthesis protein FliL, at 4,024,190 to 4,024,693 _3614 Pf1N1B4_3615 - Flagellar motor switch protein FliM, at 4,024,703 to 4,025,671 _3615 Position (kb) 4022 4023 4024Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4021.689 kb on + strandat 4021.689 kb on + strand, within Pf1N1B4_3610at 4021.746 kb on + strand, within Pf1N1B4_3610at 4021.746 kb on + strand, within Pf1N1B4_3610at 4021.747 kb on - strand, within Pf1N1B4_3610at 4021.845 kb on + strand, within Pf1N1B4_3610at 4021.887 kb on + strand, within Pf1N1B4_3610at 4021.887 kb on + strand, within Pf1N1B4_3610at 4021.888 kb on - strand, within Pf1N1B4_3610at 4021.888 kb on - strandat 4022.313 kb on + strandat 4022.325 kb on + strand, within Pf1N1B4_3611at 4022.325 kb on + strand, within Pf1N1B4_3611at 4022.464 kb on + strand, within Pf1N1B4_3611at 4022.518 kb on + strandat 4022.561 kb on + strandat 4022.571 kb on + strandat 4022.572 kb on - strandat 4022.575 kb on + strandat 4022.575 kb on + strandat 4022.575 kb on + strandat 4022.727 kb on + strandat 4022.727 kb on + strandat 4022.728 kb on - strandat 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.564 kb on + strandat 4023.564 kb on + strand, within Pf1N1B4_3612at 4023.565 kb on - strand, within Pf1N1B4_3612at 4023.565 kb on - strand, within Pf1N1B4_3612at 4023.565 kb on - strand, within Pf1N1B4_3612at 4023.565 kb on - strand, within Pf1N1B4_3612at 4023.565 kb on - strand, within Pf1N1B4_3612at 4023.954 kb on + strandat 4023.954 kb on + strandat 4023.958 kb on - strandat 4023.958 kb on - strandat 4023.958 kb on - strandat 4024.015 kb on - strandat 4024.029 kb on - strand, within Pf1N1B4_3613at 4024.029 kb on - strand, within Pf1N1B4_3613at 4024.029 kb on - strandat 4024.029 kb on - strand, within Pf1N1B4_3613at 4024.029 kb on - strand, within Pf1N1B4_3613at 4024.029 kb on - strand, within Pf1N1B4_3613at 4024.129 kb on + strandat 4024.184 kb on - strandat 4024.184 kb on - strandat 4024.195 kb on + strandat 4024.196 kb on - strandat 4024.418 kb on + strand, within Pf1N1B4_3614at 4024.418 kb on + strand, within Pf1N1B4_3614at 4024.418 kb on + strand, within Pf1N1B4_3614at 4024.419 kb on - strand, within Pf1N1B4_3614at 4024.419 kb on - strand, within Pf1N1B4_3614at 4024.419 kb on - strand, within Pf1N1B4_3614at 4024.442 kb on + strand, within Pf1N1B4_3614at 4024.443 kb on - strand, within Pf1N1B4_3614at 4024.443 kb on - strand, within Pf1N1B4_3614at 4024.443 kb on - strand, within Pf1N1B4_3614at 4024.691 kb on + strandat 4024.692 kb on - strandat 4024.814 kb on + strand, within Pf1N1B4_3615at 4024.815 kb on - strand, within Pf1N1B4_3615at 4024.815 kb on - strand, within Pf1N1B4_3615at 4024.815 kb on - strand, within Pf1N1B4_3615

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM
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4,021,689 + +1.6
4,021,689 + Pf1N1B4_3610 0.75 -0.2
4,021,746 + Pf1N1B4_3610 0.78 +0.9
4,021,746 + Pf1N1B4_3610 0.78 +0.4
4,021,747 - Pf1N1B4_3610 0.78 +0.8
4,021,845 + Pf1N1B4_3610 0.84 +1.0
4,021,887 + Pf1N1B4_3610 0.86 +0.7
4,021,887 + Pf1N1B4_3610 0.86 -0.7
4,021,888 - Pf1N1B4_3610 0.86 -0.8
4,021,888 - +1.2
4,022,313 + +1.1
4,022,325 + Pf1N1B4_3611 0.47 -0.9
4,022,325 + Pf1N1B4_3611 0.47 +0.9
4,022,464 + Pf1N1B4_3611 0.85 -2.0
4,022,518 + +0.6
4,022,561 + -0.4
4,022,571 + -0.3
4,022,572 - -2.0
4,022,575 + +0.7
4,022,575 + +0.4
4,022,575 + -0.4
4,022,727 + +0.3
4,022,727 + +0.3
4,022,728 - +1.7
4,023,564 + Pf1N1B4_3612 0.70 -1.7
4,023,564 + Pf1N1B4_3612 0.70 -1.7
4,023,564 + Pf1N1B4_3612 0.70 +1.0
4,023,564 + Pf1N1B4_3612 0.70 -0.9
4,023,564 + Pf1N1B4_3612 0.70 +1.6
4,023,564 + Pf1N1B4_3612 0.70 -1.2
4,023,564 + -0.9
4,023,564 + Pf1N1B4_3612 0.70 -0.2
4,023,565 - Pf1N1B4_3612 0.71 -1.7
4,023,565 - Pf1N1B4_3612 0.71 -0.9
4,023,565 - Pf1N1B4_3612 0.71 -0.9
4,023,565 - Pf1N1B4_3612 0.71 -0.8
4,023,565 - Pf1N1B4_3612 0.71 +1.1
4,023,954 + -0.1
4,023,954 + +1.9
4,023,958 - +0.9
4,023,958 - -1.7
4,023,958 - -0.3
4,024,015 - +1.1
4,024,029 - Pf1N1B4_3613 0.14 -0.2
4,024,029 - Pf1N1B4_3613 0.14 -0.4
4,024,029 - -0.4
4,024,029 - Pf1N1B4_3613 0.14 -0.7
4,024,029 - Pf1N1B4_3613 0.14 +1.7
4,024,029 - Pf1N1B4_3613 0.14 -0.2
4,024,129 + +1.9
4,024,184 - -1.0
4,024,184 - +0.8
4,024,195 + +1.1
4,024,196 - +0.3
4,024,418 + Pf1N1B4_3614 0.45 -0.6
4,024,418 + Pf1N1B4_3614 0.45 +0.7
4,024,418 + Pf1N1B4_3614 0.45 -0.9
4,024,419 - Pf1N1B4_3614 0.45 -1.2
4,024,419 - Pf1N1B4_3614 0.45 -0.1
4,024,419 - Pf1N1B4_3614 0.45 -1.3
4,024,442 + Pf1N1B4_3614 0.50 +0.1
4,024,443 - Pf1N1B4_3614 0.50 +0.4
4,024,443 - Pf1N1B4_3614 0.50 -0.6
4,024,443 - Pf1N1B4_3614 0.50 +0.0
4,024,691 + +3.0
4,024,692 - -0.2
4,024,814 + Pf1N1B4_3615 0.11 -0.0
4,024,815 - Pf1N1B4_3615 0.12 -1.7
4,024,815 - Pf1N1B4_3615 0.12 -1.0
4,024,815 - Pf1N1B4_3615 0.12 -2.2

Or see this region's nucleotide sequence