Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3586 and Pf1N1B4_3587 are separated by 36 nucleotides Pf1N1B4_3587 and Pf1N1B4_3588 are separated by 2 nucleotides Pf1N1B4_3588 and Pf1N1B4_3589 overlap by 4 nucleotides
Pf1N1B4_3586 - DegT/DnrJ/EryC1/StrS aminotransferase, at 3,998,444 to 3,999,607
_3586
Pf1N1B4_3587 - N-Acetylneuraminate cytidylyltransferase (EC 2.7.7.43), at 3,999,644 to 4,000,339
_3587
Pf1N1B4_3588 - N-Acetylneuraminate cytidylyltransferase (EC 2.7.7.43), at 4,000,342 to 4,001,838
_3588
Pf1N1B4_3589 - N-acetylneuraminate synthase (EC 2.5.1.56), at 4,001,835 to 4,002,887
_3589
Position (kb)
4000
4001
4002 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3999.431 kb on + strand at 3999.431 kb on + strand, within Pf1N1B4_3586 at 3999.431 kb on + strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.433 kb on + strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.456 kb on - strand, within Pf1N1B4_3586 at 3999.456 kb on - strand, within Pf1N1B4_3586 at 3999.507 kb on - strand at 3999.507 kb on - strand at 3999.509 kb on + strand at 3999.510 kb on - strand at 3999.566 kb on + strand at 3999.566 kb on + strand at 3999.566 kb on + strand at 3999.566 kb on + strand at 3999.567 kb on - strand at 3999.567 kb on - strand at 3999.567 kb on - strand at 3999.567 kb on - strand at 3999.606 kb on - strand at 3999.620 kb on + strand at 3999.620 kb on + strand at 3999.621 kb on - strand at 3999.900 kb on + strand, within Pf1N1B4_3587 at 3999.900 kb on + strand, within Pf1N1B4_3587 at 3999.900 kb on + strand, within Pf1N1B4_3587 at 3999.900 kb on + strand, within Pf1N1B4_3587 at 3999.900 kb on + strand, within Pf1N1B4_3587 at 3999.900 kb on + strand, within Pf1N1B4_3587 at 3999.901 kb on - strand, within Pf1N1B4_3587 at 3999.901 kb on - strand, within Pf1N1B4_3587 at 3999.901 kb on - strand, within Pf1N1B4_3587 at 3999.901 kb on - strand, within Pf1N1B4_3587 at 3999.901 kb on - strand, within Pf1N1B4_3587 at 3999.933 kb on - strand, within Pf1N1B4_3587 at 3999.933 kb on - strand, within Pf1N1B4_3587 at 3999.933 kb on - strand, within Pf1N1B4_3587 at 3999.948 kb on - strand, within Pf1N1B4_3587 at 4000.371 kb on + strand at 4000.419 kb on - strand at 4000.419 kb on - strand at 4000.525 kb on + strand, within Pf1N1B4_3588 at 4000.646 kb on - strand, within Pf1N1B4_3588 at 4000.646 kb on - strand, within Pf1N1B4_3588 at 4000.730 kb on - strand, within Pf1N1B4_3588 at 4000.781 kb on - strand, within Pf1N1B4_3588 at 4001.594 kb on - strand at 4001.629 kb on + strand, within Pf1N1B4_3588 at 4001.915 kb on + strand at 4001.995 kb on - strand, within Pf1N1B4_3589 at 4001.996 kb on + strand, within Pf1N1B4_3589 at 4002.225 kb on + strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.256 kb on + strand, within Pf1N1B4_3589 at 4002.257 kb on - strand, within Pf1N1B4_3589 at 4002.402 kb on + strand, within Pf1N1B4_3589 at 4002.704 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 3,999,431 + -0.4 3,999,431 + Pf1N1B4_3586 0.85 -1.6 3,999,431 + Pf1N1B4_3586 0.85 +0.9 3,999,432 - Pf1N1B4_3586 0.85 +0.2 3,999,432 - Pf1N1B4_3586 0.85 +0.8 3,999,432 - Pf1N1B4_3586 0.85 -0.0 3,999,433 + Pf1N1B4_3586 0.85 +1.3 3,999,434 - Pf1N1B4_3586 0.85 -1.2 3,999,434 - Pf1N1B4_3586 0.85 +3.2 3,999,434 - Pf1N1B4_3586 0.85 -1.8 3,999,434 - Pf1N1B4_3586 0.85 -2.0 3,999,434 - Pf1N1B4_3586 0.85 -0.4 3,999,434 - Pf1N1B4_3586 0.85 -0.8 3,999,434 - Pf1N1B4_3586 0.85 -1.2 3,999,456 - Pf1N1B4_3586 0.87 +1.4 3,999,456 - Pf1N1B4_3586 0.87 -1.3 3,999,507 - +0.4 3,999,507 - -0.4 3,999,509 + -0.1 3,999,510 - +0.3 3,999,566 + -0.6 3,999,566 + -2.2 3,999,566 + +1.2 3,999,566 + +0.0 3,999,567 - +0.2 3,999,567 - +0.8 3,999,567 - -0.7 3,999,567 - -0.4 3,999,606 - -0.4 3,999,620 + +0.8 3,999,620 + -1.8 3,999,621 - +0.6 3,999,900 + Pf1N1B4_3587 0.37 -1.2 3,999,900 + Pf1N1B4_3587 0.37 -0.2 3,999,900 + Pf1N1B4_3587 0.37 -0.3 3,999,900 + Pf1N1B4_3587 0.37 +1.5 3,999,900 + Pf1N1B4_3587 0.37 +0.4 3,999,900 + Pf1N1B4_3587 0.37 -0.6 3,999,901 - Pf1N1B4_3587 0.37 -0.2 3,999,901 - Pf1N1B4_3587 0.37 +0.6 3,999,901 - Pf1N1B4_3587 0.37 -0.3 3,999,901 - Pf1N1B4_3587 0.37 +0.2 3,999,901 - Pf1N1B4_3587 0.37 -0.0 3,999,933 - Pf1N1B4_3587 0.42 -1.2 3,999,933 - Pf1N1B4_3587 0.42 +0.4 3,999,933 - Pf1N1B4_3587 0.42 +0.7 3,999,948 - Pf1N1B4_3587 0.44 -1.0 4,000,371 + +0.1 4,000,419 - -0.9 4,000,419 - -0.4 4,000,525 + Pf1N1B4_3588 0.12 +0.0 4,000,646 - Pf1N1B4_3588 0.20 +1.2 4,000,646 - Pf1N1B4_3588 0.20 +0.8 4,000,730 - Pf1N1B4_3588 0.26 +1.6 4,000,781 - Pf1N1B4_3588 0.29 +0.4 4,001,594 - +1.2 4,001,629 + Pf1N1B4_3588 0.86 -0.8 4,001,915 + +0.2 4,001,995 - Pf1N1B4_3589 0.15 +1.6 4,001,996 + Pf1N1B4_3589 0.15 -0.2 4,002,225 + Pf1N1B4_3589 0.37 +0.2 4,002,226 - Pf1N1B4_3589 0.37 -1.7 4,002,226 - Pf1N1B4_3589 0.37 -0.3 4,002,226 - Pf1N1B4_3589 0.37 -2.7 4,002,256 + Pf1N1B4_3589 0.40 +0.0 4,002,257 - Pf1N1B4_3589 0.40 +0.3 4,002,402 + Pf1N1B4_3589 0.54 +0.4 4,002,704 - Pf1N1B4_3589 0.83 -0.0 4,002,721 - Pf1N1B4_3589 0.84 -0.2 4,002,721 - Pf1N1B4_3589 0.84 +0.4 4,002,721 - Pf1N1B4_3589 0.84 -0.8 4,002,721 - Pf1N1B4_3589 0.84 -0.7 4,002,721 - Pf1N1B4_3589 0.84 +0.2 4,002,721 - Pf1N1B4_3589 0.84 -0.3 4,002,721 - Pf1N1B4_3589 0.84 +1.8 4,002,721 - Pf1N1B4_3589 0.84 -0.4 4,002,721 - Pf1N1B4_3589 0.84 -1.3 4,002,721 - Pf1N1B4_3589 0.84 -0.8 4,002,721 - Pf1N1B4_3589 0.84 -0.5 4,002,721 - Pf1N1B4_3589 0.84 +0.1
Or see this region's nucleotide sequence