Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3296 and Pf1N1B4_3297 are separated by 314 nucleotides Pf1N1B4_3297 and Pf1N1B4_3298 are separated by 32 nucleotides Pf1N1B4_3298 and Pf1N1B4_3299 overlap by 2 nucleotides Pf1N1B4_3299 and Pf1N1B4_3300 overlap by 47 nucleotides
Pf1N1B4_3296 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 3,683,379 to 3,684,263
_3296
Pf1N1B4_3297 - Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70), at 3,684,578 to 3,685,270
_3297
Pf1N1B4_3298 - DNA or RNA helicases of superfamily II, at 3,685,303 to 3,685,521
_3298
Pf1N1B4_3299 - hypothetical protein, at 3,685,520 to 3,685,660
_3299
Pf1N1B4_3300 - Allophanate hydrolase (EC 3.5.1.54), at 3,685,614 to 3,687,392
_3300
Position (kb)
3684
3685
3686 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3683.590 kb on + strand at 3683.590 kb on + strand, within Pf1N1B4_3296 at 3683.590 kb on + strand, within Pf1N1B4_3296 at 3683.591 kb on - strand, within Pf1N1B4_3296 at 3683.591 kb on - strand, within Pf1N1B4_3296 at 3683.653 kb on + strand, within Pf1N1B4_3296 at 3683.740 kb on + strand, within Pf1N1B4_3296 at 3683.740 kb on + strand, within Pf1N1B4_3296 at 3683.831 kb on - strand at 3683.851 kb on + strand, within Pf1N1B4_3296 at 3684.088 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.214 kb on + strand at 3684.234 kb on + strand at 3684.234 kb on + strand at 3684.234 kb on + strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.236 kb on + strand at 3684.236 kb on + strand at 3684.268 kb on + strand at 3684.269 kb on - strand at 3684.326 kb on + strand at 3684.326 kb on + strand at 3684.327 kb on - strand at 3684.332 kb on + strand at 3684.333 kb on - strand at 3684.333 kb on - strand at 3684.333 kb on - strand at 3684.391 kb on + strand at 3684.391 kb on + strand at 3684.391 kb on + strand at 3684.392 kb on - strand at 3684.392 kb on - strand at 3684.392 kb on - strand at 3684.393 kb on + strand at 3684.393 kb on + strand at 3684.393 kb on + strand at 3684.394 kb on - strand at 3684.394 kb on - strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.461 kb on - strand at 3684.533 kb on - strand at 3684.579 kb on + strand at 3684.580 kb on - strand at 3684.677 kb on + strand, within Pf1N1B4_3297 at 3684.713 kb on - strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.876 kb on + strand, within Pf1N1B4_3297 at 3684.876 kb on + strand, within Pf1N1B4_3297 at 3684.877 kb on - strand at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.226 kb on - strand at 3685.226 kb on - strand at 3685.304 kb on + strand at 3685.304 kb on + strand at 3685.304 kb on + strand at 3685.305 kb on - strand at 3685.305 kb on - strand at 3685.305 kb on - strand at 3685.305 kb on - strand at 3685.305 kb on - strand at 3685.324 kb on + strand at 3685.515 kb on + strand at 3685.634 kb on - strand at 3685.634 kb on - strand at 3685.634 kb on - strand at 3685.684 kb on + strand at 3685.698 kb on - strand at 3685.731 kb on + strand at 3685.731 kb on + strand at 3685.731 kb on + strand at 3685.732 kb on - strand at 3685.732 kb on - strand at 3685.732 kb on - strand at 3685.804 kb on - strand, within Pf1N1B4_3300 at 3685.883 kb on - strand, within Pf1N1B4_3300 at 3685.883 kb on - strand, within Pf1N1B4_3300 at 3685.883 kb on - strand, within Pf1N1B4_3300 at 3686.015 kb on + strand, within Pf1N1B4_3300 at 3686.015 kb on + strand at 3686.015 kb on + strand at 3686.016 kb on - strand, within Pf1N1B4_3300 at 3686.016 kb on - strand, within Pf1N1B4_3300 at 3686.016 kb on - strand, within Pf1N1B4_3300 at 3686.016 kb on - strand, within Pf1N1B4_3300 at 3686.016 kb on - strand, within Pf1N1B4_3300 at 3686.016 kb on - strand, within Pf1N1B4_3300 at 3686.060 kb on + strand, within Pf1N1B4_3300 at 3686.080 kb on + strand, within Pf1N1B4_3300 at 3686.080 kb on + strand, within Pf1N1B4_3300 at 3686.081 kb on - strand, within Pf1N1B4_3300 at 3686.081 kb on - strand, within Pf1N1B4_3300 at 3686.081 kb on - strand, within Pf1N1B4_3300 at 3686.194 kb on + strand, within Pf1N1B4_3300 at 3686.194 kb on + strand, within Pf1N1B4_3300 at 3686.194 kb on + strand, within Pf1N1B4_3300 at 3686.194 kb on + strand, within Pf1N1B4_3300 at 3686.195 kb on - strand, within Pf1N1B4_3300 at 3686.195 kb on - strand, within Pf1N1B4_3300 at 3686.198 kb on - strand, within Pf1N1B4_3300
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 3,683,590 + +1.3 3,683,590 + Pf1N1B4_3296 0.24 -0.2 3,683,590 + Pf1N1B4_3296 0.24 +0.3 3,683,591 - Pf1N1B4_3296 0.24 -0.0 3,683,591 - Pf1N1B4_3296 0.24 +0.3 3,683,653 + Pf1N1B4_3296 0.31 +0.3 3,683,740 + Pf1N1B4_3296 0.41 -0.3 3,683,740 + Pf1N1B4_3296 0.41 +2.9 3,683,831 - +0.3 3,683,851 + Pf1N1B4_3296 0.53 -0.4 3,684,088 + Pf1N1B4_3296 0.80 -0.3 3,684,173 + Pf1N1B4_3296 0.90 +0.1 3,684,173 + Pf1N1B4_3296 0.90 +0.0 3,684,173 + Pf1N1B4_3296 0.90 +1.7 3,684,214 + +1.3 3,684,234 + -0.3 3,684,234 + -0.2 3,684,234 + -0.4 3,684,235 - +1.6 3,684,235 - -0.3 3,684,235 - -1.0 3,684,235 - -0.3 3,684,235 - -0.0 3,684,235 - +0.6 3,684,236 + +1.3 3,684,236 + -0.3 3,684,268 + +0.2 3,684,269 - +1.9 3,684,326 + -0.4 3,684,326 + -0.6 3,684,327 - +0.5 3,684,332 + +1.3 3,684,333 - -1.2 3,684,333 - +1.1 3,684,333 - +1.0 3,684,391 + +0.2 3,684,391 + -0.3 3,684,391 + -0.3 3,684,392 - -1.7 3,684,392 - -0.4 3,684,392 - -1.7 3,684,393 + -1.0 3,684,393 + -0.0 3,684,393 + -0.7 3,684,394 - -1.2 3,684,394 - +1.3 3,684,455 + -2.5 3,684,455 + +0.5 3,684,455 + +0.5 3,684,455 + -0.4 3,684,461 - +0.7 3,684,533 - -1.3 3,684,579 + -0.7 3,684,580 - +1.1 3,684,677 + Pf1N1B4_3297 0.14 -1.7 3,684,713 - Pf1N1B4_3297 0.19 -0.3 3,684,873 + Pf1N1B4_3297 0.43 -1.7 3,684,873 + Pf1N1B4_3297 0.43 -0.7 3,684,873 + Pf1N1B4_3297 0.43 -1.4 3,684,873 + Pf1N1B4_3297 0.43 -0.1 3,684,873 + Pf1N1B4_3297 0.43 -0.4 3,684,874 - Pf1N1B4_3297 0.43 -0.7 3,684,874 - Pf1N1B4_3297 0.43 -0.5 3,684,874 - Pf1N1B4_3297 0.43 -1.0 3,684,876 + Pf1N1B4_3297 0.43 -0.4 3,684,876 + Pf1N1B4_3297 0.43 +0.9 3,684,877 - -0.7 3,684,877 - Pf1N1B4_3297 0.43 -0.6 3,684,877 - Pf1N1B4_3297 0.43 -1.9 3,684,877 - Pf1N1B4_3297 0.43 -1.0 3,685,217 + +0.6 3,685,217 + +0.7 3,685,217 + -0.2 3,685,217 + +1.5 3,685,226 - +0.9 3,685,226 - -1.7 3,685,304 + +2.1 3,685,304 + +2.0 3,685,304 + -0.8 3,685,305 - +1.3 3,685,305 - +1.3 3,685,305 - -2.0 3,685,305 - +1.7 3,685,305 - -0.9 3,685,324 + +0.3 3,685,515 + +2.0 3,685,634 - -0.7 3,685,634 - +0.1 3,685,634 - +0.1 3,685,684 + -0.9 3,685,698 - +0.1 3,685,731 + -0.7 3,685,731 + -1.3 3,685,731 + -1.1 3,685,732 - +0.6 3,685,732 - -1.0 3,685,732 - +0.0 3,685,804 - Pf1N1B4_3300 0.11 +1.0 3,685,883 - Pf1N1B4_3300 0.15 -0.6 3,685,883 - Pf1N1B4_3300 0.15 +1.0 3,685,883 - Pf1N1B4_3300 0.15 -0.6 3,686,015 + Pf1N1B4_3300 0.23 -1.3 3,686,015 + +0.9 3,686,015 + -1.5 3,686,016 - Pf1N1B4_3300 0.23 +0.3 3,686,016 - Pf1N1B4_3300 0.23 -1.5 3,686,016 - Pf1N1B4_3300 0.23 +1.3 3,686,016 - Pf1N1B4_3300 0.23 +1.5 3,686,016 - Pf1N1B4_3300 0.23 +0.5 3,686,016 - Pf1N1B4_3300 0.23 -0.1 3,686,060 + Pf1N1B4_3300 0.25 -1.0 3,686,080 + Pf1N1B4_3300 0.26 +0.8 3,686,080 + Pf1N1B4_3300 0.26 -1.1 3,686,081 - Pf1N1B4_3300 0.26 +0.5 3,686,081 - Pf1N1B4_3300 0.26 -1.6 3,686,081 - Pf1N1B4_3300 0.26 -2.1 3,686,194 + Pf1N1B4_3300 0.33 +0.8 3,686,194 + Pf1N1B4_3300 0.33 -1.0 3,686,194 + Pf1N1B4_3300 0.33 +0.9 3,686,194 + Pf1N1B4_3300 0.33 +2.3 3,686,195 - Pf1N1B4_3300 0.33 +0.6 3,686,195 - Pf1N1B4_3300 0.33 +0.8 3,686,198 - Pf1N1B4_3300 0.33 +0.3
Or see this region's nucleotide sequence