Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3024 and Pf1N1B4_3025 are separated by 175 nucleotides Pf1N1B4_3025 and Pf1N1B4_3026 overlap by 11 nucleotides Pf1N1B4_3026 and Pf1N1B4_3027 are separated by 1 nucleotides
Pf1N1B4_3024 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,400,954 to 3,402,378
_3024
Pf1N1B4_3025 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,402,554 to 3,403,378
_3025
Pf1N1B4_3026 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,403,368 to 3,404,315
_3026
Pf1N1B4_3027 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,404,317 to 3,405,435
_3027
Position (kb)
3402
3403
3404 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.578 kb on - strand, within Pf1N1B4_3024 at 3401.578 kb on - strand, within Pf1N1B4_3024 at 3401.584 kb on + strand, within Pf1N1B4_3024 at 3401.585 kb on - strand, within Pf1N1B4_3024 at 3401.728 kb on + strand, within Pf1N1B4_3024 at 3401.729 kb on - strand, within Pf1N1B4_3024 at 3401.778 kb on - strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.877 kb on + strand, within Pf1N1B4_3024 at 3401.877 kb on + strand, within Pf1N1B4_3024 at 3401.877 kb on + strand, within Pf1N1B4_3024 at 3401.878 kb on - strand, within Pf1N1B4_3024 at 3401.880 kb on + strand, within Pf1N1B4_3024 at 3401.930 kb on + strand, within Pf1N1B4_3024 at 3402.002 kb on + strand, within Pf1N1B4_3024 at 3402.002 kb on + strand, within Pf1N1B4_3024 at 3402.003 kb on - strand, within Pf1N1B4_3024 at 3402.003 kb on - strand, within Pf1N1B4_3024 at 3402.101 kb on + strand, within Pf1N1B4_3024 at 3402.101 kb on + strand at 3402.102 kb on - strand, within Pf1N1B4_3024 at 3402.287 kb on - strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.486 kb on + strand at 3402.486 kb on + strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.555 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.604 kb on - strand at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.882 kb on + strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.919 kb on - strand, within Pf1N1B4_3025 at 3403.335 kb on - strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.463 kb on + strand, within Pf1N1B4_3026 at 3403.489 kb on + strand, within Pf1N1B4_3026 at 3403.490 kb on - strand, within Pf1N1B4_3026 at 3403.665 kb on + strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.732 kb on + strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.888 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.936 kb on + strand, within Pf1N1B4_3026 at 3403.967 kb on - strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.141 kb on - strand at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.228 kb on - strand at 3404.228 kb on - strand at 3404.245 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 3,401,558 + Pf1N1B4_3024 0.42 +0.4 3,401,558 + Pf1N1B4_3024 0.42 +0.8 3,401,558 + Pf1N1B4_3024 0.42 -0.1 3,401,559 - Pf1N1B4_3024 0.42 -0.2 3,401,559 - Pf1N1B4_3024 0.42 -0.4 3,401,559 - Pf1N1B4_3024 0.42 -0.6 3,401,577 + Pf1N1B4_3024 0.44 -0.0 3,401,577 + Pf1N1B4_3024 0.44 -0.6 3,401,577 + Pf1N1B4_3024 0.44 -0.4 3,401,578 - Pf1N1B4_3024 0.44 +1.4 3,401,578 - Pf1N1B4_3024 0.44 -0.2 3,401,584 + Pf1N1B4_3024 0.44 -0.5 3,401,585 - Pf1N1B4_3024 0.44 +0.7 3,401,728 + Pf1N1B4_3024 0.54 -1.2 3,401,729 - Pf1N1B4_3024 0.54 -1.0 3,401,778 - Pf1N1B4_3024 0.58 -0.9 3,401,787 + Pf1N1B4_3024 0.58 -1.0 3,401,787 + -1.0 3,401,787 + Pf1N1B4_3024 0.58 +1.4 3,401,787 + Pf1N1B4_3024 0.58 -0.2 3,401,787 + Pf1N1B4_3024 0.58 +1.4 3,401,788 - Pf1N1B4_3024 0.59 -1.0 3,401,788 - Pf1N1B4_3024 0.59 -1.1 3,401,788 - Pf1N1B4_3024 0.59 +0.4 3,401,788 - Pf1N1B4_3024 0.59 -0.2 3,401,791 + Pf1N1B4_3024 0.59 +0.8 3,401,791 + Pf1N1B4_3024 0.59 -0.2 3,401,791 + Pf1N1B4_3024 0.59 -0.6 3,401,791 + Pf1N1B4_3024 0.59 +0.1 3,401,791 + Pf1N1B4_3024 0.59 +0.1 3,401,792 - Pf1N1B4_3024 0.59 -1.0 3,401,792 - Pf1N1B4_3024 0.59 -0.2 3,401,792 - Pf1N1B4_3024 0.59 -0.8 3,401,792 - Pf1N1B4_3024 0.59 -0.7 3,401,877 + Pf1N1B4_3024 0.65 -0.5 3,401,877 + Pf1N1B4_3024 0.65 -1.3 3,401,877 + Pf1N1B4_3024 0.65 -0.0 3,401,878 - Pf1N1B4_3024 0.65 +1.3 3,401,880 + Pf1N1B4_3024 0.65 +0.2 3,401,930 + Pf1N1B4_3024 0.68 -0.4 3,402,002 + Pf1N1B4_3024 0.74 -0.8 3,402,002 + Pf1N1B4_3024 0.74 +0.9 3,402,003 - Pf1N1B4_3024 0.74 -0.2 3,402,003 - Pf1N1B4_3024 0.74 +2.0 3,402,101 + Pf1N1B4_3024 0.80 +0.2 3,402,101 + -1.0 3,402,102 - Pf1N1B4_3024 0.81 +0.1 3,402,287 - +1.8 3,402,382 + -0.4 3,402,382 + +1.4 3,402,382 + -0.0 3,402,382 + -0.3 3,402,382 + -0.6 3,402,382 + -1.2 3,402,383 - +0.1 3,402,383 - -0.7 3,402,383 - +1.2 3,402,383 - +0.4 3,402,383 - +1.0 3,402,383 - -0.6 3,402,403 + -1.1 3,402,403 + +0.2 3,402,403 + -1.1 3,402,403 + +0.1 3,402,403 + -2.7 3,402,403 + -1.3 3,402,403 + +0.4 3,402,403 + -0.6 3,402,403 + +0.2 3,402,403 + -0.6 3,402,403 + -1.4 3,402,403 + +2.1 3,402,403 + -0.5 3,402,403 + -0.3 3,402,403 + +1.4 3,402,403 + -0.0 3,402,403 + +0.3 3,402,404 - +1.4 3,402,404 - +1.4 3,402,404 - +0.5 3,402,404 - +0.0 3,402,404 - -0.0 3,402,404 - +0.8 3,402,404 - +0.1 3,402,404 - -1.4 3,402,404 - +0.7 3,402,404 - -1.3 3,402,404 - -2.2 3,402,404 - -1.6 3,402,404 - -0.6 3,402,404 - -1.2 3,402,404 - -1.0 3,402,486 + +0.3 3,402,486 + -0.4 3,402,487 - +1.4 3,402,487 - -0.2 3,402,487 - +0.4 3,402,487 - +1.4 3,402,555 + +0.1 3,402,600 + +0.1 3,402,600 + -1.2 3,402,600 + -0.1 3,402,603 + +1.0 3,402,603 + -0.3 3,402,603 + +0.5 3,402,603 + +0.3 3,402,604 - -1.4 3,402,825 + Pf1N1B4_3025 0.33 -1.0 3,402,825 + Pf1N1B4_3025 0.33 -0.2 3,402,882 + Pf1N1B4_3025 0.40 -0.4 3,402,883 - Pf1N1B4_3025 0.40 -1.4 3,402,883 - Pf1N1B4_3025 0.40 -0.6 3,402,883 - Pf1N1B4_3025 0.40 -0.7 3,402,883 - +0.0 3,402,883 - Pf1N1B4_3025 0.40 -1.3 3,402,883 - Pf1N1B4_3025 0.40 -0.9 3,402,918 + Pf1N1B4_3025 0.44 -2.3 3,402,918 + Pf1N1B4_3025 0.44 -1.3 3,402,919 - Pf1N1B4_3025 0.44 +1.0 3,403,335 - -0.2 3,403,366 + -1.2 3,403,366 + -0.6 3,403,366 + -0.0 3,403,367 - -1.6 3,403,367 - +0.4 3,403,367 - -1.8 3,403,411 + -0.2 3,403,411 + +1.4 3,403,411 + -1.2 3,403,411 + -1.2 3,403,411 + -0.6 3,403,411 + +1.4 3,403,412 - +1.4 3,403,412 - -1.1 3,403,412 - -0.2 3,403,412 - +1.9 3,403,463 + Pf1N1B4_3026 0.10 +0.4 3,403,489 + Pf1N1B4_3026 0.13 -1.0 3,403,490 - Pf1N1B4_3026 0.13 -0.2 3,403,665 + Pf1N1B4_3026 0.31 +1.6 3,403,666 - Pf1N1B4_3026 0.31 -0.7 3,403,666 - Pf1N1B4_3026 0.31 -0.6 3,403,732 + Pf1N1B4_3026 0.38 -0.2 3,403,733 - Pf1N1B4_3026 0.39 +3.0 3,403,733 - Pf1N1B4_3026 0.39 +1.4 3,403,888 + Pf1N1B4_3026 0.55 -0.1 3,403,903 + Pf1N1B4_3026 0.56 -1.0 3,403,903 + Pf1N1B4_3026 0.56 -0.5 3,403,903 + Pf1N1B4_3026 0.56 +1.0 3,403,904 - Pf1N1B4_3026 0.57 -0.4 3,403,904 - Pf1N1B4_3026 0.57 -0.1 3,403,904 - Pf1N1B4_3026 0.57 +0.1 3,403,936 + Pf1N1B4_3026 0.60 -0.2 3,403,967 - Pf1N1B4_3026 0.63 +0.7 3,403,978 + Pf1N1B4_3026 0.64 -0.2 3,403,978 + Pf1N1B4_3026 0.64 -0.4 3,403,978 + Pf1N1B4_3026 0.64 -1.0 3,403,978 + Pf1N1B4_3026 0.64 +1.0 3,403,978 + Pf1N1B4_3026 0.64 +1.0 3,403,978 + Pf1N1B4_3026 0.64 -0.6 3,403,978 + Pf1N1B4_3026 0.64 +0.6 3,403,978 + +1.4 3,403,979 - Pf1N1B4_3026 0.64 +0.1 3,403,979 - Pf1N1B4_3026 0.64 +0.8 3,403,979 - Pf1N1B4_3026 0.64 +0.0 3,403,979 - Pf1N1B4_3026 0.64 -0.6 3,403,979 - Pf1N1B4_3026 0.64 -1.6 3,403,982 - Pf1N1B4_3026 0.65 -0.2 3,403,982 - Pf1N1B4_3026 0.65 -1.4 3,404,140 + Pf1N1B4_3026 0.81 +1.1 3,404,140 + Pf1N1B4_3026 0.81 +0.2 3,404,140 + Pf1N1B4_3026 0.81 -0.5 3,404,140 + Pf1N1B4_3026 0.81 -0.2 3,404,141 - -0.6 3,404,179 + Pf1N1B4_3026 0.86 -2.4 3,404,179 + Pf1N1B4_3026 0.86 -0.6 3,404,227 + -2.5 3,404,227 + +0.8 3,404,227 + +0.2 3,404,228 - +2.4 3,404,228 - +1.5 3,404,245 + +0.4
Or see this region's nucleotide sequence