Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2328 and Pf1N1B4_2329 are separated by 74 nucleotides Pf1N1B4_2329 and Pf1N1B4_2330 are separated by 72 nucleotides Pf1N1B4_2330 and Pf1N1B4_2331 are separated by 60 nucleotides Pf1N1B4_2331 and Pf1N1B4_2332 are separated by 9 nucleotides Pf1N1B4_2332 and Pf1N1B4_2333 are separated by 116 nucleotides
Pf1N1B4_2328 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 2,648,347 to 2,649,069
_2328
Pf1N1B4_2329 - COG2363, at 2,649,144 to 2,649,515
_2329
Pf1N1B4_2330 - Sulfur carrier protein ThiS, at 2,649,588 to 2,649,806
_2330
Pf1N1B4_2331 - Thiazole biosynthesis protein ThiG, at 2,649,867 to 2,650,661
_2331
Pf1N1B4_2332 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 2,650,671 to 2,651,396
_2332
Pf1N1B4_2333 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 2,651,513 to 2,651,836
_2333
Position (kb)
2649
2650
2651 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2649.007 kb on + strand at 2649.007 kb on + strand at 2649.058 kb on + strand at 2649.069 kb on + strand at 2649.069 kb on + strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.105 kb on + strand at 2649.105 kb on + strand at 2649.106 kb on - strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.276 kb on + strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.290 kb on + strand, within Pf1N1B4_2329 at 2649.310 kb on + strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.784 kb on + strand, within Pf1N1B4_2330 at 2649.852 kb on + strand at 2649.881 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.912 kb on - strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.916 kb on - strand at 2649.934 kb on + strand at 2649.934 kb on + strand at 2649.935 kb on - strand at 2649.939 kb on - strand at 2650.083 kb on + strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.098 kb on - strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331 at 2650.591 kb on - strand at 2650.620 kb on + strand at 2650.620 kb on + strand at 2650.620 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.660 kb on - strand at 2650.660 kb on - strand at 2650.813 kb on + strand, within Pf1N1B4_2332 at 2651.029 kb on - strand, within Pf1N1B4_2332 at 2651.235 kb on + strand, within Pf1N1B4_2332 at 2651.235 kb on + strand, within Pf1N1B4_2332 at 2651.235 kb on + strand, within Pf1N1B4_2332 at 2651.394 kb on + strand at 2651.394 kb on + strand at 2651.504 kb on + strand at 2651.504 kb on + strand at 2651.505 kb on - strand at 2651.505 kb on - strand at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.622 kb on + strand, within Pf1N1B4_2333 at 2651.622 kb on + strand, within Pf1N1B4_2333 at 2651.622 kb on + strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 2,649,007 + +0.8 2,649,007 + -1.6 2,649,058 + -1.8 2,649,069 + +0.6 2,649,069 + -0.9 2,649,070 - +0.5 2,649,070 - +0.8 2,649,070 - -0.6 2,649,105 + -1.5 2,649,105 + +1.7 2,649,106 - +0.1 2,649,107 + -1.6 2,649,107 + +1.3 2,649,107 + -0.7 2,649,107 + +0.1 2,649,107 + +0.9 2,649,107 + -1.6 2,649,107 + +2.1 2,649,107 + +1.0 2,649,107 + -0.8 2,649,107 + -0.8 2,649,107 + -0.4 2,649,107 + +0.7 2,649,108 - -0.2 2,649,108 - -1.3 2,649,108 - -1.1 2,649,108 - -0.8 2,649,108 - -1.8 2,649,108 - -0.4 2,649,108 - -0.9 2,649,108 - +1.4 2,649,108 - +1.7 2,649,108 - +0.5 2,649,108 - -1.9 2,649,108 - -1.6 2,649,108 - +1.7 2,649,108 - -0.0 2,649,108 - +1.4 2,649,108 - +0.2 2,649,276 + Pf1N1B4_2329 0.35 -0.4 2,649,277 - Pf1N1B4_2329 0.36 -0.0 2,649,277 - Pf1N1B4_2329 0.36 -0.3 2,649,277 - Pf1N1B4_2329 0.36 -1.1 2,649,277 - Pf1N1B4_2329 0.36 +1.0 2,649,277 - Pf1N1B4_2329 0.36 -0.6 2,649,277 - Pf1N1B4_2329 0.36 -0.3 2,649,277 - Pf1N1B4_2329 0.36 +0.9 2,649,290 + Pf1N1B4_2329 0.39 -1.6 2,649,310 + Pf1N1B4_2329 0.45 -0.3 2,649,311 - Pf1N1B4_2329 0.45 -0.8 2,649,311 - Pf1N1B4_2329 0.45 +0.2 2,649,402 + Pf1N1B4_2329 0.69 -0.9 2,649,402 + Pf1N1B4_2329 0.69 +0.7 2,649,402 + Pf1N1B4_2329 0.69 -0.9 2,649,784 + Pf1N1B4_2330 0.89 +0.9 2,649,852 + +0.5 2,649,881 + +0.1 2,649,911 + +0.1 2,649,911 + -1.7 2,649,911 + -2.6 2,649,912 - -0.6 2,649,915 + -0.1 2,649,915 + -0.0 2,649,915 + -1.6 2,649,915 + +0.7 2,649,916 - +1.4 2,649,934 + -0.2 2,649,934 + +0.9 2,649,935 - +1.3 2,649,939 - +1.1 2,650,083 + Pf1N1B4_2331 0.27 -1.0 2,650,084 - Pf1N1B4_2331 0.27 -0.5 2,650,084 - Pf1N1B4_2331 0.27 +0.4 2,650,098 - Pf1N1B4_2331 0.29 -1.5 2,650,284 + Pf1N1B4_2331 0.52 -1.5 2,650,284 + Pf1N1B4_2331 0.52 -0.5 2,650,284 + Pf1N1B4_2331 0.52 -0.1 2,650,284 + Pf1N1B4_2331 0.52 -1.3 2,650,284 + Pf1N1B4_2331 0.52 -1.5 2,650,285 - Pf1N1B4_2331 0.53 -0.3 2,650,285 - Pf1N1B4_2331 0.53 -0.6 2,650,591 - -0.6 2,650,620 + -0.6 2,650,620 + +0.8 2,650,620 + -0.3 2,650,659 + -0.6 2,650,659 + +0.2 2,650,659 + +0.9 2,650,659 + -1.5 2,650,659 + -0.5 2,650,659 + -0.1 2,650,660 - -0.5 2,650,660 - -0.4 2,650,813 + Pf1N1B4_2332 0.20 -0.5 2,651,029 - Pf1N1B4_2332 0.49 +1.3 2,651,235 + Pf1N1B4_2332 0.78 +0.1 2,651,235 + Pf1N1B4_2332 0.78 -1.3 2,651,235 + Pf1N1B4_2332 0.78 +0.2 2,651,394 + -0.7 2,651,394 + -0.1 2,651,504 + -1.4 2,651,504 + +1.7 2,651,505 - -0.2 2,651,505 - +0.5 2,651,577 + Pf1N1B4_2333 0.20 +0.7 2,651,577 + Pf1N1B4_2333 0.20 -0.8 2,651,577 + Pf1N1B4_2333 0.20 +1.7 2,651,577 + Pf1N1B4_2333 0.20 +0.4 2,651,577 + Pf1N1B4_2333 0.20 -0.3 2,651,577 + Pf1N1B4_2333 0.20 +1.1 2,651,577 + Pf1N1B4_2333 0.20 +0.1 2,651,578 - Pf1N1B4_2333 0.20 +0.7 2,651,578 - Pf1N1B4_2333 0.20 -0.3 2,651,578 - Pf1N1B4_2333 0.20 +0.3 2,651,578 - Pf1N1B4_2333 0.20 +0.8 2,651,578 - Pf1N1B4_2333 0.20 -1.6 2,651,578 - Pf1N1B4_2333 0.20 +0.1 2,651,578 - +0.7 2,651,578 - Pf1N1B4_2333 0.20 -1.8 2,651,578 - Pf1N1B4_2333 0.20 -1.6 2,651,578 - Pf1N1B4_2333 0.20 -0.7 2,651,578 - Pf1N1B4_2333 0.20 -0.2 2,651,578 - Pf1N1B4_2333 0.20 +0.7 2,651,578 - Pf1N1B4_2333 0.20 +0.1 2,651,622 + Pf1N1B4_2333 0.34 -0.1 2,651,622 + Pf1N1B4_2333 0.34 +0.3 2,651,622 + Pf1N1B4_2333 0.34 -0.4 2,651,623 - Pf1N1B4_2333 0.34 -0.2 2,651,623 - Pf1N1B4_2333 0.34 +1.5 2,651,623 - Pf1N1B4_2333 0.34 -1.9 2,651,623 - Pf1N1B4_2333 0.34 +0.3 2,651,623 - Pf1N1B4_2333 0.34 +0.7 2,651,623 - Pf1N1B4_2333 0.34 -0.0 2,651,623 - Pf1N1B4_2333 0.34 +1.0 2,651,623 - Pf1N1B4_2333 0.34 +0.7 2,651,623 - Pf1N1B4_2333 0.34 -1.3 2,651,623 - Pf1N1B4_2333 0.34 +0.3 2,651,623 - Pf1N1B4_2333 0.34 +0.9
Or see this region's nucleotide sequence