Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2289 and Pf1N1B4_2290 are separated by 127 nucleotides Pf1N1B4_2290 and Pf1N1B4_2291 are separated by 84 nucleotides Pf1N1B4_2291 and Pf1N1B4_2292 are separated by 16 nucleotides
Pf1N1B4_2289 - Thymidylate synthase (EC 2.1.1.45), at 2,604,895 to 2,605,866
_2289
Pf1N1B4_2290 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 2,605,994 to 2,606,443
_2290
Pf1N1B4_2291 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,606,528 to 2,608,786
_2291
Pf1N1B4_2292 - Transcriptional regulators, LysR family, at 2,608,803 to 2,609,666
_2292
Position (kb)
2606
2607
2608
2609 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2605.882 kb on - strand at 2605.897 kb on - strand at 2605.966 kb on - strand at 2605.995 kb on + strand at 2605.995 kb on + strand at 2605.996 kb on - strand at 2605.996 kb on - strand at 2606.328 kb on + strand, within Pf1N1B4_2290 at 2606.328 kb on + strand, within Pf1N1B4_2290 at 2606.328 kb on + strand, within Pf1N1B4_2290 at 2606.329 kb on - strand, within Pf1N1B4_2290 at 2606.329 kb on - strand, within Pf1N1B4_2290 at 2606.329 kb on - strand, within Pf1N1B4_2290 at 2606.385 kb on + strand, within Pf1N1B4_2290 at 2606.386 kb on - strand, within Pf1N1B4_2290 at 2606.386 kb on - strand, within Pf1N1B4_2290 at 2606.475 kb on - strand at 2606.494 kb on + strand at 2606.494 kb on + strand at 2606.495 kb on - strand at 2606.495 kb on - strand at 2606.495 kb on - strand at 2606.983 kb on + strand, within Pf1N1B4_2291 at 2606.984 kb on - strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.066 kb on - strand, within Pf1N1B4_2291 at 2607.387 kb on + strand, within Pf1N1B4_2291 at 2607.387 kb on + strand, within Pf1N1B4_2291 at 2607.387 kb on + strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.506 kb on + strand, within Pf1N1B4_2291 at 2607.506 kb on + strand, within Pf1N1B4_2291 at 2607.506 kb on + strand, within Pf1N1B4_2291 at 2607.506 kb on + strand, within Pf1N1B4_2291 at 2607.506 kb on + strand, within Pf1N1B4_2291 at 2607.506 kb on + strand, within Pf1N1B4_2291 at 2607.507 kb on - strand, within Pf1N1B4_2291 at 2607.623 kb on + strand, within Pf1N1B4_2291 at 2607.623 kb on + strand, within Pf1N1B4_2291 at 2607.624 kb on - strand, within Pf1N1B4_2291 at 2607.707 kb on + strand, within Pf1N1B4_2291 at 2607.830 kb on + strand, within Pf1N1B4_2291 at 2607.830 kb on + strand, within Pf1N1B4_2291 at 2607.857 kb on + strand, within Pf1N1B4_2291 at 2607.914 kb on + strand, within Pf1N1B4_2291 at 2607.914 kb on + strand, within Pf1N1B4_2291 at 2607.914 kb on + strand, within Pf1N1B4_2291 at 2607.914 kb on + strand, within Pf1N1B4_2291 at 2607.914 kb on + strand, within Pf1N1B4_2291 at 2607.914 kb on + strand, within Pf1N1B4_2291 at 2607.915 kb on - strand at 2607.915 kb on - strand, within Pf1N1B4_2291 at 2607.945 kb on + strand, within Pf1N1B4_2291 at 2607.945 kb on + strand, within Pf1N1B4_2291 at 2607.945 kb on + strand, within Pf1N1B4_2291 at 2607.946 kb on - strand, within Pf1N1B4_2291 at 2607.946 kb on - strand, within Pf1N1B4_2291 at 2607.946 kb on - strand, within Pf1N1B4_2291 at 2607.946 kb on - strand, within Pf1N1B4_2291 at 2607.946 kb on - strand, within Pf1N1B4_2291 at 2607.946 kb on - strand, within Pf1N1B4_2291 at 2608.110 kb on - strand, within Pf1N1B4_2291 at 2608.110 kb on - strand, within Pf1N1B4_2291 at 2608.110 kb on - strand, within Pf1N1B4_2291 at 2608.223 kb on - strand, within Pf1N1B4_2291 at 2608.346 kb on - strand, within Pf1N1B4_2291 at 2608.346 kb on - strand, within Pf1N1B4_2291 at 2608.398 kb on + strand, within Pf1N1B4_2291 at 2608.398 kb on + strand, within Pf1N1B4_2291 at 2608.399 kb on - strand, within Pf1N1B4_2291 at 2608.399 kb on - strand, within Pf1N1B4_2291 at 2608.399 kb on - strand, within Pf1N1B4_2291 at 2608.436 kb on + strand, within Pf1N1B4_2291 at 2608.437 kb on - strand, within Pf1N1B4_2291 at 2608.785 kb on - strand at 2608.785 kb on - strand at 2608.785 kb on - strand at 2608.830 kb on + strand at 2608.830 kb on + strand at 2608.830 kb on + strand at 2608.830 kb on + strand at 2608.830 kb on + strand at 2608.830 kb on + strand at 2608.831 kb on - strand at 2608.831 kb on - strand at 2608.831 kb on - strand at 2608.831 kb on - strand at 2608.853 kb on + strand at 2608.853 kb on + strand at 2608.854 kb on - strand at 2608.854 kb on - strand at 2608.854 kb on - strand at 2608.856 kb on + strand at 2608.947 kb on + strand, within Pf1N1B4_2292 at 2608.947 kb on + strand, within Pf1N1B4_2292 at 2609.101 kb on - strand at 2609.101 kb on - strand, within Pf1N1B4_2292 at 2609.101 kb on - strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.103 kb on + strand, within Pf1N1B4_2292 at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.104 kb on - strand at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.104 kb on - strand, within Pf1N1B4_2292 at 2609.303 kb on + strand, within Pf1N1B4_2292 at 2609.303 kb on + strand, within Pf1N1B4_2292 at 2609.304 kb on - strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.357 kb on + strand at 2609.357 kb on + strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.358 kb on - strand, within Pf1N1B4_2292 at 2609.583 kb on + strand at 2609.583 kb on + strand at 2609.584 kb on - strand at 2609.584 kb on - strand at 2609.584 kb on - strand at 2609.589 kb on + strand at 2609.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 2,605,882 - +0.4 2,605,897 - +0.4 2,605,966 - -0.9 2,605,995 + -0.4 2,605,995 + +1.0 2,605,996 - -0.4 2,605,996 - -0.6 2,606,328 + Pf1N1B4_2290 0.74 -1.1 2,606,328 + Pf1N1B4_2290 0.74 -0.4 2,606,328 + Pf1N1B4_2290 0.74 -0.5 2,606,329 - Pf1N1B4_2290 0.74 -1.7 2,606,329 - Pf1N1B4_2290 0.74 +0.4 2,606,329 - Pf1N1B4_2290 0.74 -0.6 2,606,385 + Pf1N1B4_2290 0.87 -2.8 2,606,386 - Pf1N1B4_2290 0.87 -0.6 2,606,386 - Pf1N1B4_2290 0.87 -2.7 2,606,475 - -1.5 2,606,494 + +0.1 2,606,494 + -0.8 2,606,495 - -1.8 2,606,495 - -1.3 2,606,495 - -1.3 2,606,983 + Pf1N1B4_2291 0.20 -1.6 2,606,984 - Pf1N1B4_2291 0.20 -1.9 2,607,065 + Pf1N1B4_2291 0.24 -2.0 2,607,065 + Pf1N1B4_2291 0.24 -1.1 2,607,065 + Pf1N1B4_2291 0.24 -1.9 2,607,065 + Pf1N1B4_2291 0.24 -1.5 2,607,065 + Pf1N1B4_2291 0.24 +0.1 2,607,065 + Pf1N1B4_2291 0.24 -1.9 2,607,066 - Pf1N1B4_2291 0.24 -1.9 2,607,387 + Pf1N1B4_2291 0.38 -1.9 2,607,387 + Pf1N1B4_2291 0.38 -1.6 2,607,387 + Pf1N1B4_2291 0.38 -2.4 2,607,388 - Pf1N1B4_2291 0.38 -2.2 2,607,388 - Pf1N1B4_2291 0.38 -1.6 2,607,388 - Pf1N1B4_2291 0.38 -1.9 2,607,388 - Pf1N1B4_2291 0.38 +0.7 2,607,506 + Pf1N1B4_2291 0.43 -1.5 2,607,506 + Pf1N1B4_2291 0.43 -2.3 2,607,506 + Pf1N1B4_2291 0.43 -1.8 2,607,506 + Pf1N1B4_2291 0.43 -0.5 2,607,506 + Pf1N1B4_2291 0.43 -2.4 2,607,506 + Pf1N1B4_2291 0.43 -2.0 2,607,507 - Pf1N1B4_2291 0.43 -1.6 2,607,623 + Pf1N1B4_2291 0.48 -1.8 2,607,623 + Pf1N1B4_2291 0.48 -0.3 2,607,624 - Pf1N1B4_2291 0.49 -1.1 2,607,707 + Pf1N1B4_2291 0.52 -1.5 2,607,830 + Pf1N1B4_2291 0.58 -1.8 2,607,830 + Pf1N1B4_2291 0.58 -0.9 2,607,857 + Pf1N1B4_2291 0.59 -1.3 2,607,914 + Pf1N1B4_2291 0.61 -2.3 2,607,914 + Pf1N1B4_2291 0.61 -1.9 2,607,914 + Pf1N1B4_2291 0.61 -1.1 2,607,914 + Pf1N1B4_2291 0.61 -0.6 2,607,914 + Pf1N1B4_2291 0.61 -2.3 2,607,914 + Pf1N1B4_2291 0.61 -1.6 2,607,915 - -0.3 2,607,915 - Pf1N1B4_2291 0.61 -1.1 2,607,945 + Pf1N1B4_2291 0.63 -1.1 2,607,945 + Pf1N1B4_2291 0.63 -1.3 2,607,945 + Pf1N1B4_2291 0.63 -1.1 2,607,946 - Pf1N1B4_2291 0.63 -2.1 2,607,946 - Pf1N1B4_2291 0.63 -1.6 2,607,946 - Pf1N1B4_2291 0.63 +0.5 2,607,946 - Pf1N1B4_2291 0.63 -4.2 2,607,946 - Pf1N1B4_2291 0.63 -3.7 2,607,946 - Pf1N1B4_2291 0.63 -2.3 2,608,110 - Pf1N1B4_2291 0.70 -0.9 2,608,110 - Pf1N1B4_2291 0.70 -1.6 2,608,110 - Pf1N1B4_2291 0.70 +1.1 2,608,223 - Pf1N1B4_2291 0.75 -2.8 2,608,346 - Pf1N1B4_2291 0.80 -1.3 2,608,346 - Pf1N1B4_2291 0.80 -1.2 2,608,398 + Pf1N1B4_2291 0.83 -2.4 2,608,398 + Pf1N1B4_2291 0.83 -2.6 2,608,399 - Pf1N1B4_2291 0.83 -0.6 2,608,399 - Pf1N1B4_2291 0.83 -1.5 2,608,399 - Pf1N1B4_2291 0.83 -2.5 2,608,436 + Pf1N1B4_2291 0.84 -0.9 2,608,437 - Pf1N1B4_2291 0.85 -1.2 2,608,785 - -0.5 2,608,785 - -1.5 2,608,785 - -1.3 2,608,830 + +1.7 2,608,830 + -1.1 2,608,830 + +1.2 2,608,830 + +1.2 2,608,830 + +0.1 2,608,830 + -0.6 2,608,831 - -0.4 2,608,831 - +0.7 2,608,831 - -0.9 2,608,831 - +0.5 2,608,853 + -0.4 2,608,853 + +0.5 2,608,854 - +0.0 2,608,854 - +0.1 2,608,854 - -0.5 2,608,856 + -1.5 2,608,947 + Pf1N1B4_2292 0.17 -0.6 2,608,947 + Pf1N1B4_2292 0.17 +0.5 2,609,101 - -0.3 2,609,101 - Pf1N1B4_2292 0.34 -0.3 2,609,101 - Pf1N1B4_2292 0.34 -1.9 2,609,103 + Pf1N1B4_2292 0.35 +2.2 2,609,103 + Pf1N1B4_2292 0.35 +0.1 2,609,103 + Pf1N1B4_2292 0.35 -1.2 2,609,103 + Pf1N1B4_2292 0.35 +1.0 2,609,103 + Pf1N1B4_2292 0.35 -0.5 2,609,103 + Pf1N1B4_2292 0.35 -1.6 2,609,103 + Pf1N1B4_2292 0.35 +0.9 2,609,104 - Pf1N1B4_2292 0.35 +1.4 2,609,104 - Pf1N1B4_2292 0.35 +1.0 2,609,104 - +1.1 2,609,104 - Pf1N1B4_2292 0.35 -0.9 2,609,104 - Pf1N1B4_2292 0.35 -1.8 2,609,104 - Pf1N1B4_2292 0.35 -1.4 2,609,104 - Pf1N1B4_2292 0.35 +1.0 2,609,104 - Pf1N1B4_2292 0.35 +1.2 2,609,303 + Pf1N1B4_2292 0.58 +0.3 2,609,303 + Pf1N1B4_2292 0.58 +1.7 2,609,304 - Pf1N1B4_2292 0.58 +1.0 2,609,357 + Pf1N1B4_2292 0.64 -0.8 2,609,357 + Pf1N1B4_2292 0.64 +0.7 2,609,357 + Pf1N1B4_2292 0.64 +0.7 2,609,357 + Pf1N1B4_2292 0.64 +0.5 2,609,357 + Pf1N1B4_2292 0.64 +0.5 2,609,357 + Pf1N1B4_2292 0.64 +1.7 2,609,357 + Pf1N1B4_2292 0.64 -1.5 2,609,357 + Pf1N1B4_2292 0.64 +1.0 2,609,357 + Pf1N1B4_2292 0.64 +1.0 2,609,357 + Pf1N1B4_2292 0.64 +1.7 2,609,357 + Pf1N1B4_2292 0.64 +0.7 2,609,357 + Pf1N1B4_2292 0.64 -0.6 2,609,357 + Pf1N1B4_2292 0.64 -1.3 2,609,357 + Pf1N1B4_2292 0.64 +1.7 2,609,357 + Pf1N1B4_2292 0.64 +1.1 2,609,357 + +1.7 2,609,357 + Pf1N1B4_2292 0.64 +0.1 2,609,358 - Pf1N1B4_2292 0.64 -1.6 2,609,358 - Pf1N1B4_2292 0.64 +1.1 2,609,358 - Pf1N1B4_2292 0.64 -1.1 2,609,358 - Pf1N1B4_2292 0.64 +1.2 2,609,358 - Pf1N1B4_2292 0.64 +1.0 2,609,358 - Pf1N1B4_2292 0.64 +0.7 2,609,358 - Pf1N1B4_2292 0.64 -0.6 2,609,358 - Pf1N1B4_2292 0.64 -0.4 2,609,358 - Pf1N1B4_2292 0.64 -0.5 2,609,358 - Pf1N1B4_2292 0.64 -0.4 2,609,583 + -0.9 2,609,583 + +1.0 2,609,584 - +0.1 2,609,584 - +1.1 2,609,584 - -0.3 2,609,589 + +0.2 2,609,665 - +0.7
Or see this region's nucleotide sequence