Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2233 and Pf1N1B4_2234 are separated by 404 nucleotides Pf1N1B4_2234 and Pf1N1B4_2235 are separated by 50 nucleotides Pf1N1B4_2235 and Pf1N1B4_2236 are separated by 269 nucleotides
Pf1N1B4_2233 - Ferric iron ABC transporter, permease protein, at 2,545,311 to 2,546,894
_2233
Pf1N1B4_2234 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,547,299 to 2,548,132
_2234
Pf1N1B4_2235 - Glycine cleavage system H protein, at 2,548,183 to 2,548,569
_2235
Pf1N1B4_2236 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 2,548,839 to 2,551,712
_2236
Position (kb)
2547
2548
2549 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2546.427 kb on + strand, within Pf1N1B4_2233 at 2546.428 kb on - strand at 2546.523 kb on + strand, within Pf1N1B4_2233 at 2546.524 kb on - strand, within Pf1N1B4_2233 at 2546.609 kb on + strand, within Pf1N1B4_2233 at 2546.610 kb on - strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.650 kb on - strand, within Pf1N1B4_2233 at 2546.650 kb on - strand, within Pf1N1B4_2233 at 2546.892 kb on + strand at 2546.892 kb on + strand at 2546.905 kb on + strand at 2546.906 kb on - strand at 2546.906 kb on - strand at 2546.918 kb on + strand at 2546.918 kb on + strand at 2546.918 kb on + strand at 2546.919 kb on - strand at 2547.063 kb on - strand at 2547.073 kb on - strand at 2547.073 kb on - strand at 2547.196 kb on + strand at 2547.252 kb on - strand at 2547.255 kb on + strand at 2547.256 kb on - strand at 2547.303 kb on - strand at 2547.303 kb on - strand at 2547.354 kb on - strand at 2547.563 kb on + strand, within Pf1N1B4_2234 at 2547.564 kb on - strand, within Pf1N1B4_2234 at 2547.691 kb on + strand, within Pf1N1B4_2234 at 2547.692 kb on - strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234 at 2547.982 kb on + strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.983 kb on - strand, within Pf1N1B4_2234 at 2547.994 kb on + strand, within Pf1N1B4_2234 at 2547.994 kb on + strand, within Pf1N1B4_2234 at 2547.994 kb on + strand, within Pf1N1B4_2234 at 2548.001 kb on - strand, within Pf1N1B4_2234 at 2548.027 kb on - strand, within Pf1N1B4_2234 at 2548.131 kb on - strand at 2548.238 kb on + strand, within Pf1N1B4_2235 at 2548.238 kb on + strand, within Pf1N1B4_2235 at 2548.239 kb on - strand, within Pf1N1B4_2235 at 2548.239 kb on - strand, within Pf1N1B4_2235 at 2548.266 kb on + strand, within Pf1N1B4_2235 at 2548.266 kb on + strand, within Pf1N1B4_2235 at 2548.266 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.301 kb on + strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.302 kb on - strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.393 kb on + strand, within Pf1N1B4_2235 at 2548.394 kb on - strand, within Pf1N1B4_2235 at 2548.394 kb on - strand, within Pf1N1B4_2235 at 2548.394 kb on - strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.471 kb on + strand, within Pf1N1B4_2235 at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.546 kb on + strand at 2548.547 kb on - strand at 2548.547 kb on - strand at 2548.547 kb on - strand at 2548.567 kb on + strand at 2548.568 kb on - strand at 2548.582 kb on - strand at 2548.642 kb on + strand at 2548.642 kb on + strand at 2548.643 kb on - strand at 2548.643 kb on - strand at 2548.689 kb on + strand at 2548.689 kb on + strand at 2548.689 kb on + strand at 2548.690 kb on - strand at 2548.691 kb on + strand at 2548.691 kb on + strand at 2548.692 kb on - strand at 2548.692 kb on - strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.739 kb on + strand at 2548.782 kb on + strand at 2548.783 kb on - strand at 2548.880 kb on + strand at 2548.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 2,546,427 + Pf1N1B4_2233 0.70 -0.4 2,546,428 - +0.7 2,546,523 + Pf1N1B4_2233 0.77 +1.2 2,546,524 - Pf1N1B4_2233 0.77 -1.0 2,546,609 + Pf1N1B4_2233 0.82 -1.1 2,546,610 - Pf1N1B4_2233 0.82 -0.3 2,546,649 + Pf1N1B4_2233 0.84 +1.0 2,546,649 + Pf1N1B4_2233 0.84 +1.7 2,546,649 + Pf1N1B4_2233 0.84 +1.7 2,546,650 - Pf1N1B4_2233 0.85 -0.3 2,546,650 - Pf1N1B4_2233 0.85 +0.1 2,546,892 + -1.8 2,546,892 + -0.3 2,546,905 + +1.3 2,546,906 - -0.6 2,546,906 - -0.6 2,546,918 + +0.0 2,546,918 + +0.0 2,546,918 + -0.6 2,546,919 - +0.7 2,547,063 - +1.7 2,547,073 - +1.1 2,547,073 - -0.9 2,547,196 + -1.4 2,547,252 - +0.1 2,547,255 + -0.3 2,547,256 - +0.5 2,547,303 - -0.6 2,547,303 - +1.3 2,547,354 - -0.8 2,547,563 + Pf1N1B4_2234 0.32 -1.9 2,547,564 - Pf1N1B4_2234 0.32 +0.1 2,547,691 + Pf1N1B4_2234 0.47 -0.1 2,547,692 - Pf1N1B4_2234 0.47 -2.2 2,547,879 + Pf1N1B4_2234 0.70 -1.0 2,547,879 + Pf1N1B4_2234 0.70 -1.3 2,547,982 + Pf1N1B4_2234 0.82 +0.2 2,547,983 - Pf1N1B4_2234 0.82 +0.5 2,547,983 - Pf1N1B4_2234 0.82 +1.0 2,547,983 - Pf1N1B4_2234 0.82 +0.4 2,547,983 - Pf1N1B4_2234 0.82 -2.2 2,547,994 + Pf1N1B4_2234 0.83 -0.5 2,547,994 + Pf1N1B4_2234 0.83 -0.4 2,547,994 + Pf1N1B4_2234 0.83 +0.2 2,548,001 - Pf1N1B4_2234 0.84 -1.2 2,548,027 - Pf1N1B4_2234 0.87 -1.5 2,548,131 - +0.4 2,548,238 + Pf1N1B4_2235 0.14 +2.3 2,548,238 + Pf1N1B4_2235 0.14 -1.9 2,548,239 - Pf1N1B4_2235 0.14 -1.0 2,548,239 - Pf1N1B4_2235 0.14 -0.3 2,548,266 + Pf1N1B4_2235 0.21 -1.3 2,548,266 + Pf1N1B4_2235 0.21 -0.2 2,548,266 + Pf1N1B4_2235 0.21 -1.5 2,548,301 + Pf1N1B4_2235 0.30 -0.1 2,548,301 + Pf1N1B4_2235 0.30 +1.0 2,548,301 + Pf1N1B4_2235 0.30 +1.1 2,548,301 + Pf1N1B4_2235 0.30 -0.2 2,548,301 + Pf1N1B4_2235 0.30 +0.3 2,548,301 + Pf1N1B4_2235 0.30 -1.1 2,548,301 + Pf1N1B4_2235 0.30 -1.0 2,548,301 + Pf1N1B4_2235 0.30 +0.6 2,548,301 + Pf1N1B4_2235 0.30 -1.8 2,548,301 + Pf1N1B4_2235 0.30 -0.4 2,548,301 + Pf1N1B4_2235 0.30 +1.1 2,548,301 + Pf1N1B4_2235 0.30 -0.5 2,548,301 + Pf1N1B4_2235 0.30 +1.7 2,548,301 + Pf1N1B4_2235 0.30 -0.9 2,548,301 + Pf1N1B4_2235 0.30 -0.9 2,548,301 + Pf1N1B4_2235 0.30 +0.0 2,548,301 + Pf1N1B4_2235 0.30 +0.3 2,548,301 + Pf1N1B4_2235 0.30 +0.9 2,548,301 + Pf1N1B4_2235 0.30 +0.7 2,548,301 + Pf1N1B4_2235 0.30 -0.3 2,548,301 + Pf1N1B4_2235 0.30 -1.5 2,548,301 + Pf1N1B4_2235 0.30 +2.3 2,548,301 + Pf1N1B4_2235 0.30 +0.3 2,548,301 + Pf1N1B4_2235 0.30 +0.9 2,548,301 + Pf1N1B4_2235 0.30 -0.7 2,548,301 + Pf1N1B4_2235 0.30 -1.6 2,548,301 + Pf1N1B4_2235 0.30 +0.9 2,548,302 - Pf1N1B4_2235 0.31 +1.0 2,548,302 - Pf1N1B4_2235 0.31 +0.7 2,548,302 - Pf1N1B4_2235 0.31 -1.8 2,548,302 - Pf1N1B4_2235 0.31 -0.5 2,548,302 - Pf1N1B4_2235 0.31 +1.3 2,548,302 - Pf1N1B4_2235 0.31 -1.7 2,548,302 - Pf1N1B4_2235 0.31 +0.1 2,548,393 + Pf1N1B4_2235 0.54 +0.8 2,548,393 + Pf1N1B4_2235 0.54 +0.7 2,548,393 + Pf1N1B4_2235 0.54 -0.3 2,548,393 + Pf1N1B4_2235 0.54 +1.0 2,548,393 + Pf1N1B4_2235 0.54 +0.4 2,548,393 + Pf1N1B4_2235 0.54 +0.3 2,548,393 + Pf1N1B4_2235 0.54 -0.1 2,548,393 + Pf1N1B4_2235 0.54 +0.4 2,548,393 + Pf1N1B4_2235 0.54 +1.7 2,548,393 + Pf1N1B4_2235 0.54 -0.6 2,548,393 + Pf1N1B4_2235 0.54 +0.1 2,548,393 + Pf1N1B4_2235 0.54 -1.3 2,548,394 - Pf1N1B4_2235 0.55 -0.9 2,548,394 - Pf1N1B4_2235 0.55 -1.5 2,548,394 - Pf1N1B4_2235 0.55 -0.1 2,548,471 + Pf1N1B4_2235 0.74 -0.3 2,548,471 + Pf1N1B4_2235 0.74 +0.6 2,548,471 + Pf1N1B4_2235 0.74 +0.7 2,548,471 + Pf1N1B4_2235 0.74 +1.1 2,548,471 + Pf1N1B4_2235 0.74 +0.9 2,548,546 + -1.0 2,548,546 + +1.7 2,548,546 + -0.2 2,548,546 + -0.9 2,548,546 + +1.3 2,548,546 + +1.3 2,548,546 + -0.6 2,548,546 + -0.7 2,548,546 + -1.6 2,548,547 - -0.6 2,548,547 - -0.5 2,548,547 - -0.4 2,548,567 + -0.4 2,548,568 - -0.8 2,548,582 - +0.0 2,548,642 + -0.6 2,548,642 + +0.0 2,548,643 - +1.3 2,548,643 - +0.3 2,548,689 + -0.3 2,548,689 + +3.3 2,548,689 + -0.7 2,548,690 - +1.0 2,548,691 + -0.6 2,548,691 + -0.1 2,548,692 - -0.1 2,548,692 - +1.7 2,548,739 + +0.2 2,548,739 + +0.7 2,548,739 + +3.7 2,548,739 + +1.0 2,548,739 + +0.6 2,548,782 + +0.1 2,548,783 - +1.3 2,548,880 + -0.3 2,548,880 + +0.1
Or see this region's nucleotide sequence