Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2192 and Pf1N1B4_2193 overlap by 4 nucleotides Pf1N1B4_2193 and Pf1N1B4_2194 are separated by 50 nucleotides Pf1N1B4_2194 and Pf1N1B4_2195 overlap by 1 nucleotides Pf1N1B4_2195 and Pf1N1B4_2196 are separated by 255 nucleotides
Pf1N1B4_2192 - Twin-arginine translocation protein TatB, at 2,503,926 to 2,505,059
_2192
Pf1N1B4_2193 - hypothetical protein, at 2,505,056 to 2,505,520
_2193
Pf1N1B4_2194 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 2,505,571 to 2,507,481
_2194
Pf1N1B4_2195 - Small-conductance mechanosensitive channel, at 2,507,481 to 2,508,062
_2195
Pf1N1B4_2196 - Homoserine/homoserine lactone efflux protein, at 2,508,318 to 2,508,950
_2196
Position (kb)
2505
2506
2507
2508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2504.720 kb on + strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.820 kb on - strand, within Pf1N1B4_2192 at 2504.820 kb on - strand, within Pf1N1B4_2192 at 2504.974 kb on + strand at 2505.057 kb on + strand at 2505.057 kb on + strand at 2505.057 kb on + strand at 2505.057 kb on + strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.236 kb on + strand, within Pf1N1B4_2193 at 2505.237 kb on - strand, within Pf1N1B4_2193 at 2505.237 kb on - strand, within Pf1N1B4_2193 at 2505.237 kb on - strand, within Pf1N1B4_2193 at 2505.345 kb on - strand, within Pf1N1B4_2193 at 2505.382 kb on + strand, within Pf1N1B4_2193 at 2505.382 kb on + strand, within Pf1N1B4_2193 at 2505.383 kb on - strand, within Pf1N1B4_2193 at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.484 kb on - strand at 2505.484 kb on - strand at 2505.484 kb on - strand at 2505.484 kb on - strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.526 kb on + strand at 2505.526 kb on + strand at 2505.526 kb on + strand at 2505.554 kb on + strand at 2505.570 kb on + strand at 2505.570 kb on + strand at 2505.596 kb on + strand at 2505.596 kb on + strand at 2505.597 kb on - strand at 2505.621 kb on - strand at 2505.817 kb on + strand, within Pf1N1B4_2194 at 2505.818 kb on - strand, within Pf1N1B4_2194 at 2505.818 kb on - strand, within Pf1N1B4_2194 at 2506.010 kb on + strand, within Pf1N1B4_2194 at 2506.010 kb on + strand, within Pf1N1B4_2194 at 2506.011 kb on - strand, within Pf1N1B4_2194 at 2506.011 kb on - strand, within Pf1N1B4_2194 at 2506.011 kb on - strand, within Pf1N1B4_2194 at 2506.147 kb on - strand, within Pf1N1B4_2194 at 2506.147 kb on - strand at 2506.217 kb on + strand, within Pf1N1B4_2194 at 2506.249 kb on + strand, within Pf1N1B4_2194 at 2506.315 kb on + strand, within Pf1N1B4_2194 at 2506.315 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2507.050 kb on - strand, within Pf1N1B4_2194 at 2507.050 kb on - strand, within Pf1N1B4_2194 at 2507.062 kb on + strand, within Pf1N1B4_2194 at 2507.063 kb on - strand, within Pf1N1B4_2194 at 2507.113 kb on + strand, within Pf1N1B4_2194 at 2507.114 kb on - strand, within Pf1N1B4_2194 at 2507.135 kb on - strand, within Pf1N1B4_2194 at 2507.335 kb on + strand at 2507.335 kb on + strand at 2507.335 kb on + strand at 2507.335 kb on + strand at 2507.336 kb on - strand at 2507.568 kb on + strand, within Pf1N1B4_2195 at 2507.568 kb on + strand, within Pf1N1B4_2195 at 2507.569 kb on - strand at 2507.592 kb on - strand, within Pf1N1B4_2195 at 2507.828 kb on + strand, within Pf1N1B4_2195 at 2507.925 kb on + strand, within Pf1N1B4_2195 at 2507.926 kb on - strand, within Pf1N1B4_2195 at 2508.060 kb on + strand at 2508.060 kb on + strand at 2508.060 kb on + strand at 2508.060 kb on + strand at 2508.214 kb on + strand at 2508.278 kb on + strand at 2508.278 kb on + strand at 2508.278 kb on + strand at 2508.278 kb on + strand at 2508.279 kb on - strand at 2508.283 kb on + strand at 2508.283 kb on + strand at 2508.284 kb on - strand at 2508.284 kb on - strand at 2508.284 kb on - strand at 2508.284 kb on - strand at 2508.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 2,504,720 + Pf1N1B4_2192 0.70 +1.4 2,504,721 - Pf1N1B4_2192 0.70 +0.7 2,504,721 - Pf1N1B4_2192 0.70 +0.4 2,504,819 + Pf1N1B4_2192 0.79 -0.1 2,504,819 + Pf1N1B4_2192 0.79 +0.8 2,504,819 + Pf1N1B4_2192 0.79 -0.7 2,504,819 + Pf1N1B4_2192 0.79 +1.5 2,504,820 - Pf1N1B4_2192 0.79 -0.5 2,504,820 - Pf1N1B4_2192 0.79 -0.6 2,504,974 + -0.5 2,505,057 + +0.5 2,505,057 + +0.7 2,505,057 + -0.6 2,505,057 + +1.9 2,505,058 - +0.0 2,505,058 - -0.1 2,505,058 - +0.3 2,505,058 - +0.4 2,505,058 - -1.5 2,505,236 + Pf1N1B4_2193 0.39 +1.3 2,505,237 - Pf1N1B4_2193 0.39 -0.2 2,505,237 - Pf1N1B4_2193 0.39 +0.7 2,505,237 - Pf1N1B4_2193 0.39 -1.6 2,505,345 - Pf1N1B4_2193 0.62 -0.3 2,505,382 + Pf1N1B4_2193 0.70 +0.7 2,505,382 + Pf1N1B4_2193 0.70 +0.7 2,505,383 - Pf1N1B4_2193 0.70 -1.3 2,505,483 + +0.1 2,505,483 + -1.9 2,505,483 + -1.5 2,505,483 + +0.7 2,505,483 + +1.1 2,505,483 + +0.0 2,505,483 + +0.4 2,505,483 + +1.7 2,505,483 + -0.6 2,505,483 + +0.7 2,505,483 + -2.0 2,505,483 + -0.9 2,505,483 + -0.0 2,505,484 - +1.1 2,505,484 - -0.9 2,505,484 - +0.4 2,505,484 - +1.7 2,505,520 + +0.1 2,505,520 + -0.4 2,505,520 + +0.1 2,505,520 + +0.8 2,505,520 + +0.2 2,505,520 + -1.1 2,505,520 + +1.3 2,505,520 + -0.3 2,505,520 + -1.3 2,505,520 + -0.6 2,505,520 + -2.6 2,505,520 + +0.2 2,505,520 + +0.9 2,505,521 - -0.9 2,505,521 - +1.3 2,505,521 - +0.4 2,505,521 - -1.5 2,505,521 - -0.6 2,505,521 - +0.1 2,505,521 - +1.7 2,505,521 - +0.1 2,505,521 - +2.7 2,505,521 - +0.7 2,505,521 - -0.9 2,505,521 - +1.2 2,505,521 - +0.1 2,505,521 - +0.3 2,505,521 - -0.6 2,505,521 - -0.3 2,505,526 + -0.0 2,505,526 + -1.3 2,505,526 + +1.7 2,505,554 + +1.2 2,505,570 + +0.3 2,505,570 + +0.6 2,505,596 + -1.0 2,505,596 + +0.1 2,505,597 - -1.2 2,505,621 - -1.5 2,505,817 + Pf1N1B4_2194 0.13 +1.1 2,505,818 - Pf1N1B4_2194 0.13 -0.0 2,505,818 - Pf1N1B4_2194 0.13 -0.2 2,506,010 + Pf1N1B4_2194 0.23 +0.4 2,506,010 + Pf1N1B4_2194 0.23 +0.7 2,506,011 - Pf1N1B4_2194 0.23 +0.1 2,506,011 - Pf1N1B4_2194 0.23 +1.7 2,506,011 - Pf1N1B4_2194 0.23 +0.5 2,506,147 - Pf1N1B4_2194 0.30 -1.1 2,506,147 - +0.1 2,506,217 + Pf1N1B4_2194 0.34 -0.3 2,506,249 + Pf1N1B4_2194 0.35 -0.6 2,506,315 + Pf1N1B4_2194 0.39 +1.1 2,506,315 + Pf1N1B4_2194 0.39 -0.1 2,506,354 + Pf1N1B4_2194 0.41 +0.8 2,506,354 + Pf1N1B4_2194 0.41 +2.1 2,506,354 + Pf1N1B4_2194 0.41 -0.9 2,506,354 + Pf1N1B4_2194 0.41 +1.7 2,506,355 - Pf1N1B4_2194 0.41 +0.9 2,506,355 - Pf1N1B4_2194 0.41 +0.1 2,506,355 - Pf1N1B4_2194 0.41 +0.5 2,506,355 - Pf1N1B4_2194 0.41 -1.5 2,507,050 - Pf1N1B4_2194 0.77 +0.6 2,507,050 - Pf1N1B4_2194 0.77 +1.4 2,507,062 + Pf1N1B4_2194 0.78 -0.3 2,507,063 - Pf1N1B4_2194 0.78 -1.1 2,507,113 + Pf1N1B4_2194 0.81 +1.7 2,507,114 - Pf1N1B4_2194 0.81 -0.0 2,507,135 - Pf1N1B4_2194 0.82 -0.1 2,507,335 + +0.6 2,507,335 + +0.4 2,507,335 + -0.4 2,507,335 + +1.1 2,507,336 - +0.4 2,507,568 + Pf1N1B4_2195 0.15 +0.4 2,507,568 + Pf1N1B4_2195 0.15 -0.4 2,507,569 - +0.4 2,507,592 - Pf1N1B4_2195 0.19 +1.1 2,507,828 + Pf1N1B4_2195 0.60 +0.7 2,507,925 + Pf1N1B4_2195 0.76 +1.0 2,507,926 - Pf1N1B4_2195 0.76 +1.7 2,508,060 + -0.7 2,508,060 + +1.0 2,508,060 + +1.1 2,508,060 + +1.1 2,508,214 + +1.0 2,508,278 + -0.1 2,508,278 + -1.2 2,508,278 + +2.0 2,508,278 + -0.3 2,508,279 - -0.7 2,508,283 + +0.9 2,508,283 + -1.4 2,508,284 - -0.6 2,508,284 - -0.3 2,508,284 - +1.2 2,508,284 - +0.4 2,508,284 - -0.6
Or see this region's nucleotide sequence