Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_209 and Pf1N1B4_210 are separated by 141 nucleotides Pf1N1B4_210 and Pf1N1B4_211 overlap by 1 nucleotides Pf1N1B4_211 and Pf1N1B4_212 overlap by 25 nucleotides
Pf1N1B4_209 - TETRATRICOPEPTIDE REPEAT FAMILY PROTEIN, at 256,833 to 257,780
_209
Pf1N1B4_210 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 257,922 to 259,661
_210
Pf1N1B4_211 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 259,661 to 260,527
_211
Pf1N1B4_212 - Thiol:disulfide interchange protein DsbG precursor, at 260,503 to 261,270
_212
Position (kb) 257
258
259
260 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 256.929 kb on + strand at 256.929 kb on + strand at 256.962 kb on + strand, within Pf1N1B4_209 at 256.962 kb on + strand, within Pf1N1B4_209 at 256.962 kb on + strand, within Pf1N1B4_209 at 257.005 kb on - strand, within Pf1N1B4_209 at 257.057 kb on + strand, within Pf1N1B4_209 at 257.083 kb on - strand, within Pf1N1B4_209 at 257.113 kb on - strand, within Pf1N1B4_209 at 257.113 kb on - strand, within Pf1N1B4_209 at 257.161 kb on + strand, within Pf1N1B4_209 at 257.161 kb on + strand at 257.161 kb on + strand, within Pf1N1B4_209 at 257.162 kb on - strand, within Pf1N1B4_209 at 257.162 kb on - strand, within Pf1N1B4_209 at 257.162 kb on - strand, within Pf1N1B4_209 at 257.214 kb on + strand, within Pf1N1B4_209 at 257.221 kb on + strand, within Pf1N1B4_209 at 257.222 kb on - strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.280 kb on + strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.281 kb on - strand, within Pf1N1B4_209 at 257.284 kb on + strand, within Pf1N1B4_209 at 257.284 kb on + strand, within Pf1N1B4_209 at 257.284 kb on + strand, within Pf1N1B4_209 at 257.284 kb on + strand at 257.284 kb on + strand, within Pf1N1B4_209 at 257.285 kb on - strand, within Pf1N1B4_209 at 257.298 kb on + strand, within Pf1N1B4_209 at 257.299 kb on - strand, within Pf1N1B4_209 at 257.299 kb on - strand, within Pf1N1B4_209 at 257.486 kb on - strand, within Pf1N1B4_209 at 257.486 kb on - strand, within Pf1N1B4_209 at 257.486 kb on - strand at 257.557 kb on - strand, within Pf1N1B4_209 at 257.634 kb on + strand at 257.634 kb on + strand, within Pf1N1B4_209 at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.692 kb on + strand at 257.707 kb on + strand at 257.708 kb on - strand at 257.763 kb on + strand at 257.763 kb on + strand at 257.763 kb on + strand at 257.841 kb on - strand at 257.841 kb on - strand at 258.017 kb on - strand at 258.017 kb on - strand at 258.100 kb on - strand, within Pf1N1B4_210 at 258.102 kb on + strand, within Pf1N1B4_210 at 258.102 kb on + strand, within Pf1N1B4_210 at 258.102 kb on + strand, within Pf1N1B4_210 at 258.103 kb on - strand, within Pf1N1B4_210 at 258.103 kb on - strand, within Pf1N1B4_210 at 258.103 kb on - strand, within Pf1N1B4_210 at 258.109 kb on - strand, within Pf1N1B4_210 at 258.147 kb on + strand, within Pf1N1B4_210 at 258.147 kb on + strand, within Pf1N1B4_210 at 258.148 kb on - strand, within Pf1N1B4_210 at 258.324 kb on + strand, within Pf1N1B4_210 at 258.324 kb on + strand at 258.324 kb on + strand, within Pf1N1B4_210 at 258.324 kb on + strand, within Pf1N1B4_210 at 258.324 kb on + strand, within Pf1N1B4_210 at 258.325 kb on - strand, within Pf1N1B4_210 at 258.325 kb on - strand, within Pf1N1B4_210 at 258.325 kb on - strand, within Pf1N1B4_210 at 258.325 kb on - strand, within Pf1N1B4_210 at 258.325 kb on - strand, within Pf1N1B4_210 at 258.325 kb on - strand, within Pf1N1B4_210 at 258.328 kb on + strand, within Pf1N1B4_210 at 258.355 kb on + strand, within Pf1N1B4_210 at 258.355 kb on + strand, within Pf1N1B4_210 at 258.356 kb on - strand, within Pf1N1B4_210 at 258.550 kb on - strand, within Pf1N1B4_210 at 258.550 kb on - strand, within Pf1N1B4_210 at 258.550 kb on - strand, within Pf1N1B4_210 at 258.550 kb on - strand, within Pf1N1B4_210 at 258.573 kb on + strand at 258.573 kb on + strand, within Pf1N1B4_210 at 258.574 kb on - strand, within Pf1N1B4_210 at 258.574 kb on - strand, within Pf1N1B4_210 at 258.574 kb on - strand, within Pf1N1B4_210 at 258.574 kb on - strand, within Pf1N1B4_210 at 258.855 kb on + strand at 258.855 kb on + strand, within Pf1N1B4_210 at 258.855 kb on + strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.926 kb on - strand at 259.108 kb on - strand at 259.184 kb on - strand, within Pf1N1B4_210 at 259.251 kb on + strand, within Pf1N1B4_210 at 259.381 kb on - strand, within Pf1N1B4_210 at 259.384 kb on - strand, within Pf1N1B4_210 at 259.384 kb on - strand, within Pf1N1B4_210 at 259.465 kb on + strand at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.529 kb on + strand at 259.529 kb on + strand at 259.530 kb on - strand at 259.530 kb on - strand at 259.530 kb on - strand at 259.672 kb on + strand at 259.699 kb on + strand at 259.700 kb on - strand at 259.847 kb on + strand, within Pf1N1B4_211 at 259.848 kb on - strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.950 kb on - strand, within Pf1N1B4_211 at 259.998 kb on + strand, within Pf1N1B4_211 at 259.998 kb on + strand, within Pf1N1B4_211 at 259.999 kb on - strand, within Pf1N1B4_211 at 260.044 kb on + strand, within Pf1N1B4_211 at 260.044 kb on + strand, within Pf1N1B4_211 at 260.045 kb on - strand, within Pf1N1B4_211 at 260.045 kb on - strand, within Pf1N1B4_211 at 260.045 kb on - strand, within Pf1N1B4_211 at 260.048 kb on + strand, within Pf1N1B4_211 at 260.048 kb on + strand, within Pf1N1B4_211 at 260.049 kb on - strand, within Pf1N1B4_211 at 260.049 kb on - strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.107 kb on - strand, within Pf1N1B4_211 at 260.107 kb on - strand, within Pf1N1B4_211 at 260.107 kb on - strand, within Pf1N1B4_211 at 260.120 kb on + strand, within Pf1N1B4_211 at 260.120 kb on + strand, within Pf1N1B4_211 at 260.365 kb on + strand, within Pf1N1B4_211 at 260.366 kb on - strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.377 kb on + strand, within Pf1N1B4_211
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 256,929 + -1.5 256,929 + +0.9 256,962 + Pf1N1B4_209 0.14 +0.8 256,962 + Pf1N1B4_209 0.14 +0.5 256,962 + Pf1N1B4_209 0.14 -0.3 257,005 - Pf1N1B4_209 0.18 -2.5 257,057 + Pf1N1B4_209 0.24 +1.3 257,083 - Pf1N1B4_209 0.26 +0.7 257,113 - Pf1N1B4_209 0.30 +0.1 257,113 - Pf1N1B4_209 0.30 +0.1 257,161 + Pf1N1B4_209 0.35 -0.9 257,161 + +2.1 257,161 + Pf1N1B4_209 0.35 -0.2 257,162 - Pf1N1B4_209 0.35 +0.1 257,162 - Pf1N1B4_209 0.35 +1.8 257,162 - Pf1N1B4_209 0.35 +0.4 257,214 + Pf1N1B4_209 0.40 +0.8 257,221 + Pf1N1B4_209 0.41 +0.3 257,222 - Pf1N1B4_209 0.41 -0.6 257,280 + Pf1N1B4_209 0.47 +1.4 257,280 + Pf1N1B4_209 0.47 -0.4 257,280 + Pf1N1B4_209 0.47 +1.7 257,280 + Pf1N1B4_209 0.47 -1.9 257,280 + Pf1N1B4_209 0.47 +0.8 257,280 + Pf1N1B4_209 0.47 -1.2 257,280 + Pf1N1B4_209 0.47 -0.1 257,280 + Pf1N1B4_209 0.47 -0.1 257,280 + Pf1N1B4_209 0.47 +0.6 257,281 - Pf1N1B4_209 0.47 +0.1 257,281 - Pf1N1B4_209 0.47 +0.3 257,281 - Pf1N1B4_209 0.47 -0.2 257,281 - Pf1N1B4_209 0.47 -0.5 257,281 - Pf1N1B4_209 0.47 -1.5 257,281 - Pf1N1B4_209 0.47 -0.1 257,281 - Pf1N1B4_209 0.47 +0.6 257,281 - Pf1N1B4_209 0.47 +0.8 257,281 - Pf1N1B4_209 0.47 -1.2 257,284 + Pf1N1B4_209 0.48 -1.5 257,284 + Pf1N1B4_209 0.48 -0.2 257,284 + Pf1N1B4_209 0.48 -0.6 257,284 + +0.7 257,284 + Pf1N1B4_209 0.48 +1.4 257,285 - Pf1N1B4_209 0.48 +1.1 257,298 + Pf1N1B4_209 0.49 +0.1 257,299 - Pf1N1B4_209 0.49 -0.9 257,299 - Pf1N1B4_209 0.49 +1.5 257,486 - Pf1N1B4_209 0.69 +1.1 257,486 - Pf1N1B4_209 0.69 +0.2 257,486 - -1.2 257,557 - Pf1N1B4_209 0.76 +0.8 257,634 + -0.5 257,634 + Pf1N1B4_209 0.84 -0.7 257,692 + +0.3 257,692 + -0.5 257,692 + -0.2 257,692 + +0.1 257,692 + -0.2 257,692 + +0.1 257,692 + -0.4 257,692 + -0.4 257,692 + -1.4 257,692 + -1.1 257,692 + +0.1 257,692 + -0.9 257,692 + +0.1 257,707 + -1.1 257,708 - +0.3 257,763 + +0.1 257,763 + +0.1 257,763 + -0.4 257,841 - -0.3 257,841 - -0.9 258,017 - +0.5 258,017 - -2.8 258,100 - Pf1N1B4_210 0.10 -0.7 258,102 + Pf1N1B4_210 0.10 +0.3 258,102 + Pf1N1B4_210 0.10 -1.1 258,102 + Pf1N1B4_210 0.10 +0.4 258,103 - Pf1N1B4_210 0.10 -1.9 258,103 - Pf1N1B4_210 0.10 -0.2 258,103 - Pf1N1B4_210 0.10 -1.4 258,109 - Pf1N1B4_210 0.11 -0.9 258,147 + Pf1N1B4_210 0.13 -1.7 258,147 + Pf1N1B4_210 0.13 +0.1 258,148 - Pf1N1B4_210 0.13 -1.3 258,324 + Pf1N1B4_210 0.23 -0.6 258,324 + +0.8 258,324 + Pf1N1B4_210 0.23 +0.4 258,324 + Pf1N1B4_210 0.23 +0.9 258,324 + Pf1N1B4_210 0.23 -0.9 258,325 - Pf1N1B4_210 0.23 -2.1 258,325 - Pf1N1B4_210 0.23 -0.9 258,325 - Pf1N1B4_210 0.23 -0.5 258,325 - Pf1N1B4_210 0.23 +0.8 258,325 - Pf1N1B4_210 0.23 +0.1 258,325 - Pf1N1B4_210 0.23 -1.9 258,328 + Pf1N1B4_210 0.23 -0.6 258,355 + Pf1N1B4_210 0.25 +2.7 258,355 + Pf1N1B4_210 0.25 -0.8 258,356 - Pf1N1B4_210 0.25 -1.2 258,550 - Pf1N1B4_210 0.36 -1.1 258,550 - Pf1N1B4_210 0.36 +0.0 258,550 - Pf1N1B4_210 0.36 -2.2 258,550 - Pf1N1B4_210 0.36 -0.0 258,573 + +0.1 258,573 + Pf1N1B4_210 0.37 -0.1 258,574 - Pf1N1B4_210 0.37 -2.2 258,574 - Pf1N1B4_210 0.37 -0.5 258,574 - Pf1N1B4_210 0.37 -1.3 258,574 - Pf1N1B4_210 0.37 -1.5 258,855 + +0.1 258,855 + Pf1N1B4_210 0.54 +1.1 258,855 + Pf1N1B4_210 0.54 -3.7 258,856 - Pf1N1B4_210 0.54 -2.1 258,856 - Pf1N1B4_210 0.54 -0.4 258,856 - Pf1N1B4_210 0.54 -0.8 258,856 - Pf1N1B4_210 0.54 -1.9 258,856 - Pf1N1B4_210 0.54 -1.3 258,926 - -0.5 259,108 - -2.1 259,184 - Pf1N1B4_210 0.73 -0.9 259,251 + Pf1N1B4_210 0.76 -0.0 259,381 - Pf1N1B4_210 0.84 -1.7 259,384 - Pf1N1B4_210 0.84 -2.1 259,384 - Pf1N1B4_210 0.84 -2.6 259,465 + -0.9 259,466 - Pf1N1B4_210 0.89 -0.8 259,466 - Pf1N1B4_210 0.89 -0.7 259,466 - Pf1N1B4_210 0.89 -1.5 259,466 - Pf1N1B4_210 0.89 -0.7 259,466 - Pf1N1B4_210 0.89 -1.2 259,529 + -1.9 259,529 + +0.1 259,530 - -2.5 259,530 - -1.5 259,530 - -1.5 259,672 + -0.9 259,699 + -1.1 259,700 - +0.2 259,847 + Pf1N1B4_211 0.21 -0.3 259,848 - Pf1N1B4_211 0.22 -1.8 259,949 + Pf1N1B4_211 0.33 -0.1 259,949 + Pf1N1B4_211 0.33 -2.2 259,949 + Pf1N1B4_211 0.33 +0.1 259,949 + Pf1N1B4_211 0.33 -1.2 259,949 + Pf1N1B4_211 0.33 -1.2 259,950 - Pf1N1B4_211 0.33 -1.6 259,998 + Pf1N1B4_211 0.39 -0.4 259,998 + Pf1N1B4_211 0.39 -1.1 259,999 - Pf1N1B4_211 0.39 -0.3 260,044 + Pf1N1B4_211 0.44 -0.3 260,044 + Pf1N1B4_211 0.44 -0.6 260,045 - Pf1N1B4_211 0.44 -1.1 260,045 - Pf1N1B4_211 0.44 -3.0 260,045 - Pf1N1B4_211 0.44 -2.5 260,048 + Pf1N1B4_211 0.45 -1.1 260,048 + Pf1N1B4_211 0.45 -0.7 260,049 - Pf1N1B4_211 0.45 -1.1 260,049 - Pf1N1B4_211 0.45 -2.6 260,106 + Pf1N1B4_211 0.51 -0.5 260,106 + Pf1N1B4_211 0.51 -1.7 260,106 + Pf1N1B4_211 0.51 -0.8 260,106 + Pf1N1B4_211 0.51 +0.1 260,106 + Pf1N1B4_211 0.51 -3.0 260,106 + Pf1N1B4_211 0.51 -0.5 260,106 + Pf1N1B4_211 0.51 -1.0 260,106 + Pf1N1B4_211 0.51 -1.7 260,107 - Pf1N1B4_211 0.51 -2.0 260,107 - Pf1N1B4_211 0.51 -1.3 260,107 - Pf1N1B4_211 0.51 -0.9 260,120 + Pf1N1B4_211 0.53 -1.6 260,120 + Pf1N1B4_211 0.53 -2.1 260,365 + Pf1N1B4_211 0.81 -0.4 260,366 - Pf1N1B4_211 0.81 -0.9 260,375 + Pf1N1B4_211 0.82 -3.6 260,375 + Pf1N1B4_211 0.82 -1.4 260,375 + Pf1N1B4_211 0.82 +0.1 260,375 + Pf1N1B4_211 0.82 +0.4 260,375 + Pf1N1B4_211 0.82 -1.4 260,375 + Pf1N1B4_211 0.82 -0.3 260,375 + Pf1N1B4_211 0.82 -0.6 260,375 + Pf1N1B4_211 0.82 -0.9 260,375 + Pf1N1B4_211 0.82 -2.2 260,375 + Pf1N1B4_211 0.82 -1.2 260,375 + Pf1N1B4_211 0.82 -0.7 260,375 + Pf1N1B4_211 0.82 -1.0 260,375 + Pf1N1B4_211 0.82 +0.1 260,375 + Pf1N1B4_211 0.82 +0.4 260,375 + Pf1N1B4_211 0.82 -2.2 260,375 + Pf1N1B4_211 0.82 -0.7 260,376 - Pf1N1B4_211 0.82 -2.5 260,376 - Pf1N1B4_211 0.82 -0.7 260,376 - Pf1N1B4_211 0.82 -1.5 260,376 - Pf1N1B4_211 0.82 -3.5 260,376 - Pf1N1B4_211 0.82 -2.0 260,376 - Pf1N1B4_211 0.82 -1.6 260,376 - Pf1N1B4_211 0.82 -0.5 260,376 - Pf1N1B4_211 0.82 -1.4 260,376 - Pf1N1B4_211 0.82 -0.4 260,376 - Pf1N1B4_211 0.82 -0.8 260,376 - Pf1N1B4_211 0.82 -0.7 260,376 - Pf1N1B4_211 0.82 -0.9 260,376 - Pf1N1B4_211 0.82 -0.5 260,376 - Pf1N1B4_211 0.82 -0.8 260,376 - Pf1N1B4_211 0.82 -1.4 260,376 - Pf1N1B4_211 0.82 -1.8 260,376 - Pf1N1B4_211 0.82 -0.5 260,377 + Pf1N1B4_211 0.83 -0.1
Or see this region's nucleotide sequence