Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1962 and Pf1N1B4_1963 are separated by 14 nucleotides Pf1N1B4_1963 and Pf1N1B4_1964 are separated by 0 nucleotides Pf1N1B4_1964 and Pf1N1B4_1965 are separated by 3 nucleotides Pf1N1B4_1965 and Pf1N1B4_1966 overlap by 4 nucleotides
Pf1N1B4_1962 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 2,242,531 to 2,243,769
_1962
Pf1N1B4_1963 - O-antigen export system permease protein RfbD, at 2,243,784 to 2,244,581
_1963
Pf1N1B4_1964 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 2,244,582 to 2,246,024
_1964
Pf1N1B4_1965 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 2,246,028 to 2,246,999
_1965
Pf1N1B4_1966 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,246,996 to 2,247,754
_1966
Position (kb)
2244
2245
2246
2247 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2243.589 kb on + strand, within Pf1N1B4_1962 at 2243.590 kb on - strand, within Pf1N1B4_1962 at 2243.590 kb on - strand, within Pf1N1B4_1962 at 2243.590 kb on - strand, within Pf1N1B4_1962 at 2243.653 kb on + strand at 2243.654 kb on - strand at 2243.654 kb on - strand at 2243.654 kb on - strand at 2243.738 kb on + strand at 2243.747 kb on + strand at 2243.747 kb on + strand at 2243.748 kb on - strand at 2243.748 kb on - strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.773 kb on + strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.774 kb on - strand at 2243.920 kb on + strand, within Pf1N1B4_1963 at 2243.921 kb on - strand, within Pf1N1B4_1963 at 2243.921 kb on - strand at 2244.364 kb on + strand, within Pf1N1B4_1963 at 2244.364 kb on + strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.365 kb on - strand, within Pf1N1B4_1963 at 2244.373 kb on + strand, within Pf1N1B4_1963 at 2244.373 kb on + strand, within Pf1N1B4_1963 at 2244.374 kb on - strand, within Pf1N1B4_1963 at 2244.374 kb on - strand, within Pf1N1B4_1963 at 2244.374 kb on - strand, within Pf1N1B4_1963 at 2244.436 kb on + strand, within Pf1N1B4_1963 at 2244.436 kb on + strand at 2244.436 kb on + strand, within Pf1N1B4_1963 at 2244.437 kb on - strand, within Pf1N1B4_1963 at 2244.437 kb on - strand, within Pf1N1B4_1963 at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.637 kb on + strand at 2244.638 kb on - strand at 2244.857 kb on + strand, within Pf1N1B4_1964 at 2244.858 kb on - strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.898 kb on + strand at 2244.898 kb on + strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.899 kb on - strand, within Pf1N1B4_1964 at 2244.938 kb on - strand, within Pf1N1B4_1964 at 2245.047 kb on - strand, within Pf1N1B4_1964 at 2245.250 kb on - strand, within Pf1N1B4_1964 at 2245.460 kb on - strand, within Pf1N1B4_1964 at 2245.460 kb on - strand, within Pf1N1B4_1964 at 2245.460 kb on - strand, within Pf1N1B4_1964 at 2245.837 kb on + strand, within Pf1N1B4_1964 at 2245.837 kb on + strand, within Pf1N1B4_1964 at 2245.837 kb on + strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.838 kb on - strand at 2245.838 kb on - strand, within Pf1N1B4_1964 at 2245.840 kb on + strand, within Pf1N1B4_1964 at 2245.840 kb on + strand, within Pf1N1B4_1964 at 2245.910 kb on + strand at 2245.911 kb on - strand at 2245.911 kb on - strand at 2245.911 kb on - strand at 2245.911 kb on - strand at 2246.029 kb on + strand at 2246.030 kb on - strand at 2246.030 kb on - strand at 2246.151 kb on + strand, within Pf1N1B4_1965 at 2246.152 kb on - strand, within Pf1N1B4_1965 at 2246.191 kb on + strand, within Pf1N1B4_1965 at 2246.191 kb on + strand, within Pf1N1B4_1965 at 2246.192 kb on - strand, within Pf1N1B4_1965 at 2246.201 kb on - strand, within Pf1N1B4_1965 at 2246.201 kb on - strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.275 kb on + strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.276 kb on - strand, within Pf1N1B4_1965 at 2246.323 kb on + strand, within Pf1N1B4_1965 at 2246.323 kb on + strand, within Pf1N1B4_1965 at 2246.324 kb on - strand, within Pf1N1B4_1965 at 2246.533 kb on + strand, within Pf1N1B4_1965 at 2246.635 kb on + strand, within Pf1N1B4_1965 at 2246.635 kb on + strand, within Pf1N1B4_1965 at 2246.635 kb on + strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.636 kb on - strand, within Pf1N1B4_1965 at 2246.764 kb on + strand, within Pf1N1B4_1965 at 2246.764 kb on + strand, within Pf1N1B4_1965 at 2246.765 kb on - strand, within Pf1N1B4_1965 at 2246.765 kb on - strand, within Pf1N1B4_1965 at 2246.837 kb on - strand, within Pf1N1B4_1965 at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.920 kb on + strand at 2246.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 2,243,589 + Pf1N1B4_1962 0.85 -1.6 2,243,590 - Pf1N1B4_1962 0.85 -2.0 2,243,590 - Pf1N1B4_1962 0.85 +0.8 2,243,590 - Pf1N1B4_1962 0.85 -0.3 2,243,653 + -0.1 2,243,654 - +1.4 2,243,654 - -1.8 2,243,654 - +1.4 2,243,738 + -0.8 2,243,747 + -1.9 2,243,747 + +0.9 2,243,748 - +0.2 2,243,748 - +1.5 2,243,773 + -1.9 2,243,773 + -1.0 2,243,773 + +1.1 2,243,773 + -0.2 2,243,773 + -0.2 2,243,773 + -0.5 2,243,773 + -0.3 2,243,773 + +0.3 2,243,773 + +0.2 2,243,773 + -1.5 2,243,774 - +2.4 2,243,774 - -0.2 2,243,774 - -0.8 2,243,774 - +0.6 2,243,774 - -0.2 2,243,774 - -0.5 2,243,774 - +0.8 2,243,774 - +1.4 2,243,774 - -1.2 2,243,920 + Pf1N1B4_1963 0.17 +0.2 2,243,921 - Pf1N1B4_1963 0.17 +0.0 2,243,921 - +0.2 2,244,364 + Pf1N1B4_1963 0.73 +0.2 2,244,364 + Pf1N1B4_1963 0.73 +1.3 2,244,365 - Pf1N1B4_1963 0.73 -0.4 2,244,365 - Pf1N1B4_1963 0.73 -0.6 2,244,365 - Pf1N1B4_1963 0.73 -0.2 2,244,365 - Pf1N1B4_1963 0.73 +0.8 2,244,365 - Pf1N1B4_1963 0.73 +0.2 2,244,373 + Pf1N1B4_1963 0.74 +0.8 2,244,373 + Pf1N1B4_1963 0.74 -1.0 2,244,374 - Pf1N1B4_1963 0.74 +0.2 2,244,374 - Pf1N1B4_1963 0.74 +0.2 2,244,374 - Pf1N1B4_1963 0.74 -0.8 2,244,436 + Pf1N1B4_1963 0.82 -0.5 2,244,436 + +1.2 2,244,436 + Pf1N1B4_1963 0.82 +0.8 2,244,437 - Pf1N1B4_1963 0.82 +0.5 2,244,437 - Pf1N1B4_1963 0.82 -1.0 2,244,637 + -1.4 2,244,637 + +0.8 2,244,637 + -1.0 2,244,637 + -0.9 2,244,637 + +1.2 2,244,638 - -0.2 2,244,857 + Pf1N1B4_1964 0.19 -0.9 2,244,858 - Pf1N1B4_1964 0.19 +1.8 2,244,898 + Pf1N1B4_1964 0.22 +0.8 2,244,898 + Pf1N1B4_1964 0.22 -2.1 2,244,898 + Pf1N1B4_1964 0.22 -1.4 2,244,898 + Pf1N1B4_1964 0.22 -1.2 2,244,898 + Pf1N1B4_1964 0.22 -0.2 2,244,898 + +0.8 2,244,898 + Pf1N1B4_1964 0.22 -0.2 2,244,899 - Pf1N1B4_1964 0.22 -1.0 2,244,899 - Pf1N1B4_1964 0.22 +0.5 2,244,899 - Pf1N1B4_1964 0.22 -0.7 2,244,899 - Pf1N1B4_1964 0.22 +0.8 2,244,899 - Pf1N1B4_1964 0.22 -1.5 2,244,899 - Pf1N1B4_1964 0.22 +0.8 2,244,899 - Pf1N1B4_1964 0.22 +0.6 2,244,938 - Pf1N1B4_1964 0.25 +3.4 2,245,047 - Pf1N1B4_1964 0.32 -1.0 2,245,250 - Pf1N1B4_1964 0.46 -0.2 2,245,460 - Pf1N1B4_1964 0.61 -0.4 2,245,460 - Pf1N1B4_1964 0.61 -1.2 2,245,460 - Pf1N1B4_1964 0.61 -1.4 2,245,837 + Pf1N1B4_1964 0.87 +0.2 2,245,837 + Pf1N1B4_1964 0.87 -0.9 2,245,837 + Pf1N1B4_1964 0.87 -0.0 2,245,838 - Pf1N1B4_1964 0.87 +2.1 2,245,838 - Pf1N1B4_1964 0.87 -1.5 2,245,838 - Pf1N1B4_1964 0.87 -2.7 2,245,838 - Pf1N1B4_1964 0.87 +1.8 2,245,838 - Pf1N1B4_1964 0.87 +0.0 2,245,838 - Pf1N1B4_1964 0.87 -0.8 2,245,838 - -0.8 2,245,838 - Pf1N1B4_1964 0.87 -0.5 2,245,840 + Pf1N1B4_1964 0.87 -1.0 2,245,840 + Pf1N1B4_1964 0.87 -1.8 2,245,910 + +0.3 2,245,911 - -0.5 2,245,911 - -2.1 2,245,911 - -1.4 2,245,911 - +0.8 2,246,029 + -0.2 2,246,030 - -0.6 2,246,030 - -0.4 2,246,151 + Pf1N1B4_1965 0.13 -1.2 2,246,152 - Pf1N1B4_1965 0.13 -1.0 2,246,191 + Pf1N1B4_1965 0.17 -1.0 2,246,191 + Pf1N1B4_1965 0.17 +0.4 2,246,192 - Pf1N1B4_1965 0.17 -0.4 2,246,201 - Pf1N1B4_1965 0.18 -1.4 2,246,201 - Pf1N1B4_1965 0.18 -0.8 2,246,275 + Pf1N1B4_1965 0.25 -0.3 2,246,275 + Pf1N1B4_1965 0.25 +1.3 2,246,275 + Pf1N1B4_1965 0.25 -0.5 2,246,275 + Pf1N1B4_1965 0.25 -0.4 2,246,275 + Pf1N1B4_1965 0.25 -0.4 2,246,275 + Pf1N1B4_1965 0.25 -1.7 2,246,275 + Pf1N1B4_1965 0.25 +1.2 2,246,275 + Pf1N1B4_1965 0.25 -0.4 2,246,275 + Pf1N1B4_1965 0.25 -0.7 2,246,275 + Pf1N1B4_1965 0.25 -0.2 2,246,276 - Pf1N1B4_1965 0.26 +0.9 2,246,276 - Pf1N1B4_1965 0.26 -0.2 2,246,276 - Pf1N1B4_1965 0.26 -0.2 2,246,276 - Pf1N1B4_1965 0.26 -0.2 2,246,276 - Pf1N1B4_1965 0.26 -0.9 2,246,276 - Pf1N1B4_1965 0.26 -0.2 2,246,323 + Pf1N1B4_1965 0.30 -0.4 2,246,323 + Pf1N1B4_1965 0.30 -0.9 2,246,324 - Pf1N1B4_1965 0.30 -0.4 2,246,533 + Pf1N1B4_1965 0.52 -1.1 2,246,635 + Pf1N1B4_1965 0.62 -1.2 2,246,635 + Pf1N1B4_1965 0.62 -0.1 2,246,635 + Pf1N1B4_1965 0.62 +0.8 2,246,636 - Pf1N1B4_1965 0.63 -0.2 2,246,636 - Pf1N1B4_1965 0.63 +1.2 2,246,636 - Pf1N1B4_1965 0.63 -1.0 2,246,636 - Pf1N1B4_1965 0.63 -0.2 2,246,636 - Pf1N1B4_1965 0.63 +0.4 2,246,764 + Pf1N1B4_1965 0.76 +0.7 2,246,764 + Pf1N1B4_1965 0.76 +1.3 2,246,765 - Pf1N1B4_1965 0.76 -1.3 2,246,765 - Pf1N1B4_1965 0.76 -0.3 2,246,837 - Pf1N1B4_1965 0.83 -1.6 2,246,918 + +0.3 2,246,918 + -0.8 2,246,918 + -0.0 2,246,918 + -1.0 2,246,918 + +1.4 2,246,919 - -1.1 2,246,919 - +0.4 2,246,919 - -1.0 2,246,919 - -1.7 2,246,919 - +0.8 2,246,919 - +0.2 2,246,919 - -0.2 2,246,919 - -1.4 2,246,919 - +1.1 2,246,920 + -0.4 2,246,950 + -0.3
Or see this region's nucleotide sequence