Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1373 and Pf1N1B4_1374 are separated by 28 nucleotides Pf1N1B4_1374 and Pf1N1B4_1375 are separated by 201 nucleotides Pf1N1B4_1375 and Pf1N1B4_1376 are separated by 118 nucleotides
Pf1N1B4_1373 - Urease accessory protein UreG, at 1,571,368 to 1,571,982
_1373
Pf1N1B4_1374 - HupE-UreJ family metal transporter, at 1,572,011 to 1,572,583
_1374
Pf1N1B4_1375 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 1,572,785 to 1,573,912
_1375
Pf1N1B4_1376 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,574,031 to 1,574,798
_1376
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1571.837 kb on + strand, within Pf1N1B4_1373 at 1571.850 kb on - strand, within Pf1N1B4_1373 at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1572.007 kb on - strand at 1572.007 kb on - strand at 1572.226 kb on + strand, within Pf1N1B4_1374 at 1572.227 kb on - strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.635 kb on + strand at 1572.635 kb on + strand at 1572.743 kb on + strand at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.993 kb on - strand, within Pf1N1B4_1375 at 1572.993 kb on - strand, within Pf1N1B4_1375 at 1573.103 kb on + strand, within Pf1N1B4_1375 at 1573.103 kb on + strand, within Pf1N1B4_1375 at 1573.104 kb on - strand, within Pf1N1B4_1375 at 1573.151 kb on + strand, within Pf1N1B4_1375 at 1573.151 kb on + strand, within Pf1N1B4_1375 at 1573.152 kb on - strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.419 kb on + strand, within Pf1N1B4_1375 at 1573.420 kb on - strand, within Pf1N1B4_1375 at 1573.495 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.597 kb on + strand, within Pf1N1B4_1375 at 1573.709 kb on + strand, within Pf1N1B4_1375 at 1573.709 kb on + strand, within Pf1N1B4_1375 at 1573.710 kb on - strand, within Pf1N1B4_1375 at 1573.788 kb on + strand, within Pf1N1B4_1375 at 1573.789 kb on - strand, within Pf1N1B4_1375 at 1573.789 kb on - strand, within Pf1N1B4_1375 at 1573.789 kb on - strand, within Pf1N1B4_1375 at 1573.805 kb on + strand at 1573.806 kb on - strand at 1573.901 kb on + strand at 1573.902 kb on - strand at 1573.917 kb on + strand at 1573.917 kb on + strand at 1573.917 kb on + strand at 1573.918 kb on - strand at 1573.918 kb on - strand at 1573.918 kb on - strand at 1573.918 kb on - strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.925 kb on + strand at 1574.032 kb on + strand at 1574.032 kb on + strand at 1574.042 kb on + strand at 1574.043 kb on - strand at 1574.185 kb on + strand, within Pf1N1B4_1376 at 1574.185 kb on + strand, within Pf1N1B4_1376 at 1574.186 kb on - strand, within Pf1N1B4_1376 at 1574.186 kb on - strand, within Pf1N1B4_1376 at 1574.186 kb on - strand, within Pf1N1B4_1376 at 1574.198 kb on - strand, within Pf1N1B4_1376 at 1574.198 kb on - strand, within Pf1N1B4_1376 at 1574.344 kb on + strand, within Pf1N1B4_1376 at 1574.344 kb on + strand, within Pf1N1B4_1376 at 1574.344 kb on + strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.368 kb on + strand, within Pf1N1B4_1376 at 1574.368 kb on + strand, within Pf1N1B4_1376 at 1574.368 kb on + strand, within Pf1N1B4_1376 at 1574.369 kb on - strand, within Pf1N1B4_1376 at 1574.369 kb on - strand, within Pf1N1B4_1376 at 1574.369 kb on - strand, within Pf1N1B4_1376 at 1574.452 kb on + strand, within Pf1N1B4_1376 at 1574.452 kb on + strand at 1574.452 kb on + strand, within Pf1N1B4_1376 at 1574.452 kb on + strand at 1574.452 kb on + strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.780 kb on + strand at 1574.780 kb on + strand at 1574.780 kb on + strand at 1574.781 kb on - strand at 1574.781 kb on - strand at 1574.838 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 1,571,837 + Pf1N1B4_1373 0.76 -0.7 1,571,850 - Pf1N1B4_1373 0.78 -0.0 1,571,990 + -0.2 1,571,990 + -1.2 1,571,990 + -0.4 1,571,990 + -0.9 1,571,991 - -0.3 1,571,991 - +1.3 1,571,991 - +0.1 1,571,991 - -0.6 1,571,991 - -1.8 1,571,991 - -1.0 1,571,991 - -1.2 1,571,991 - +1.0 1,572,007 - -0.0 1,572,007 - +0.1 1,572,226 + Pf1N1B4_1374 0.38 -1.2 1,572,227 - Pf1N1B4_1374 0.38 +0.3 1,572,437 + Pf1N1B4_1374 0.74 -0.4 1,572,437 + Pf1N1B4_1374 0.74 -0.6 1,572,437 + Pf1N1B4_1374 0.74 +0.9 1,572,437 + Pf1N1B4_1374 0.74 -0.2 1,572,635 + +1.0 1,572,635 + -0.2 1,572,743 + -1.8 1,572,992 + Pf1N1B4_1375 0.18 +0.1 1,572,992 + Pf1N1B4_1375 0.18 -0.3 1,572,992 + Pf1N1B4_1375 0.18 -0.8 1,572,992 + Pf1N1B4_1375 0.18 -0.6 1,572,992 + Pf1N1B4_1375 0.18 -1.9 1,572,993 - Pf1N1B4_1375 0.18 +0.6 1,572,993 - Pf1N1B4_1375 0.18 +1.2 1,573,103 + Pf1N1B4_1375 0.28 -0.0 1,573,103 + Pf1N1B4_1375 0.28 -0.7 1,573,104 - Pf1N1B4_1375 0.28 +0.2 1,573,151 + Pf1N1B4_1375 0.32 -0.8 1,573,151 + Pf1N1B4_1375 0.32 -1.9 1,573,152 - Pf1N1B4_1375 0.33 +1.8 1,573,280 + Pf1N1B4_1375 0.44 -1.5 1,573,280 + Pf1N1B4_1375 0.44 -0.9 1,573,280 + Pf1N1B4_1375 0.44 +0.1 1,573,280 + Pf1N1B4_1375 0.44 -2.0 1,573,281 - Pf1N1B4_1375 0.44 -0.3 1,573,281 - Pf1N1B4_1375 0.44 -0.9 1,573,281 - Pf1N1B4_1375 0.44 -0.2 1,573,281 - Pf1N1B4_1375 0.44 -1.0 1,573,392 + Pf1N1B4_1375 0.54 +0.1 1,573,392 + Pf1N1B4_1375 0.54 -0.0 1,573,392 + Pf1N1B4_1375 0.54 -0.4 1,573,392 + Pf1N1B4_1375 0.54 -2.2 1,573,392 + Pf1N1B4_1375 0.54 -1.3 1,573,393 - Pf1N1B4_1375 0.54 -2.4 1,573,393 - Pf1N1B4_1375 0.54 -0.9 1,573,393 - Pf1N1B4_1375 0.54 -1.1 1,573,393 - Pf1N1B4_1375 0.54 -0.8 1,573,393 - Pf1N1B4_1375 0.54 +0.6 1,573,393 - Pf1N1B4_1375 0.54 -1.2 1,573,419 + Pf1N1B4_1375 0.56 -1.2 1,573,420 - Pf1N1B4_1375 0.56 -1.6 1,573,495 + Pf1N1B4_1375 0.63 +0.6 1,573,529 + Pf1N1B4_1375 0.66 -0.8 1,573,529 + Pf1N1B4_1375 0.66 -0.9 1,573,529 + Pf1N1B4_1375 0.66 -2.2 1,573,530 - Pf1N1B4_1375 0.66 -1.1 1,573,530 - Pf1N1B4_1375 0.66 -0.4 1,573,530 - Pf1N1B4_1375 0.66 -2.0 1,573,530 - Pf1N1B4_1375 0.66 -0.7 1,573,530 - Pf1N1B4_1375 0.66 -0.8 1,573,597 + Pf1N1B4_1375 0.72 -0.2 1,573,709 + Pf1N1B4_1375 0.82 -0.4 1,573,709 + Pf1N1B4_1375 0.82 -0.8 1,573,710 - Pf1N1B4_1375 0.82 -1.4 1,573,788 + Pf1N1B4_1375 0.89 +0.6 1,573,789 - Pf1N1B4_1375 0.89 -1.2 1,573,789 - Pf1N1B4_1375 0.89 +0.2 1,573,789 - Pf1N1B4_1375 0.89 -1.6 1,573,805 + -1.8 1,573,806 - +0.8 1,573,901 + +0.6 1,573,902 - -0.9 1,573,917 + -1.4 1,573,917 + -1.6 1,573,917 + -0.7 1,573,918 - -1.2 1,573,918 - -1.2 1,573,918 - +0.4 1,573,918 - -0.7 1,573,921 + -0.2 1,573,921 + +2.0 1,573,921 + -0.8 1,573,921 + +1.7 1,573,921 + +0.1 1,573,922 - +0.6 1,573,922 - -1.2 1,573,922 - -1.4 1,573,922 - +0.2 1,573,922 - -1.4 1,573,922 - +1.0 1,573,922 - -0.1 1,573,922 - +0.3 1,573,922 - +0.3 1,573,922 - -0.0 1,573,922 - -0.3 1,573,922 - +0.0 1,573,922 - -0.2 1,573,922 - -2.6 1,573,922 - +0.5 1,573,922 - +0.6 1,573,922 - +0.2 1,573,922 - -0.6 1,573,922 - +0.8 1,573,925 + -1.2 1,574,032 + -0.9 1,574,032 + +1.0 1,574,042 + -0.6 1,574,043 - +0.5 1,574,185 + Pf1N1B4_1376 0.20 -1.6 1,574,185 + Pf1N1B4_1376 0.20 -0.6 1,574,186 - Pf1N1B4_1376 0.20 +1.1 1,574,186 - Pf1N1B4_1376 0.20 +0.8 1,574,186 - Pf1N1B4_1376 0.20 -0.9 1,574,198 - Pf1N1B4_1376 0.22 -1.0 1,574,198 - Pf1N1B4_1376 0.22 -0.0 1,574,344 + Pf1N1B4_1376 0.41 -0.2 1,574,344 + Pf1N1B4_1376 0.41 -1.0 1,574,344 + Pf1N1B4_1376 0.41 -0.5 1,574,345 - Pf1N1B4_1376 0.41 -1.2 1,574,345 - Pf1N1B4_1376 0.41 -1.1 1,574,345 - Pf1N1B4_1376 0.41 +0.7 1,574,345 - Pf1N1B4_1376 0.41 +0.1 1,574,345 - Pf1N1B4_1376 0.41 +0.6 1,574,368 + Pf1N1B4_1376 0.44 +0.1 1,574,368 + Pf1N1B4_1376 0.44 -0.0 1,574,368 + Pf1N1B4_1376 0.44 -0.2 1,574,369 - Pf1N1B4_1376 0.44 +0.8 1,574,369 - Pf1N1B4_1376 0.44 -0.2 1,574,369 - Pf1N1B4_1376 0.44 +0.8 1,574,452 + Pf1N1B4_1376 0.55 -2.1 1,574,452 + -0.8 1,574,452 + Pf1N1B4_1376 0.55 +0.2 1,574,452 + +0.8 1,574,452 + Pf1N1B4_1376 0.55 -1.0 1,574,453 - Pf1N1B4_1376 0.55 +0.4 1,574,453 - Pf1N1B4_1376 0.55 +0.9 1,574,453 - Pf1N1B4_1376 0.55 -0.1 1,574,453 - Pf1N1B4_1376 0.55 +1.3 1,574,453 - Pf1N1B4_1376 0.55 -0.8 1,574,453 - Pf1N1B4_1376 0.55 -0.4 1,574,453 - Pf1N1B4_1376 0.55 -0.7 1,574,780 + -0.0 1,574,780 + +0.8 1,574,780 + -2.0 1,574,781 - -0.1 1,574,781 - -1.6 1,574,838 + +0.2
Or see this region's nucleotide sequence