Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1235 and Pf1N1B4_1236 overlap by 4 nucleotides Pf1N1B4_1236 and Pf1N1B4_5178 are separated by 154 nucleotides Pf1N1B4_5178 and Pf1N1B4_5179 are separated by 171 nucleotides
Pf1N1B4_1235 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 1,402,162 to 1,405,851
_1235
Pf1N1B4_1236 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 1,405,848 to 1,409,300
_1236
Pf1N1B4_5178 - 5S RNA, at 1,409,455 to 1,409,574
_5178
Pf1N1B4_5179 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 1,409,746 to 1,412,637
_5179
Position (kb)
1405
1406
1407
1408
1409
1410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1404.998 kb on - strand, within Pf1N1B4_1235 at 1405.377 kb on + strand, within Pf1N1B4_1235 at 1405.629 kb on + strand at 1405.749 kb on - strand at 1405.761 kb on + strand at 1405.761 kb on + strand at 1405.761 kb on + strand at 1406.030 kb on - strand at 1406.030 kb on - strand at 1406.098 kb on - strand at 1406.225 kb on + strand, within Pf1N1B4_1236 at 1406.403 kb on + strand, within Pf1N1B4_1236 at 1406.580 kb on + strand, within Pf1N1B4_1236 at 1406.739 kb on + strand, within Pf1N1B4_1236 at 1406.740 kb on - strand, within Pf1N1B4_1236 at 1406.797 kb on + strand, within Pf1N1B4_1236 at 1406.874 kb on + strand, within Pf1N1B4_1236 at 1406.874 kb on + strand, within Pf1N1B4_1236 at 1406.875 kb on - strand, within Pf1N1B4_1236 at 1406.896 kb on + strand, within Pf1N1B4_1236 at 1406.897 kb on - strand, within Pf1N1B4_1236 at 1407.067 kb on + strand, within Pf1N1B4_1236 at 1407.067 kb on + strand, within Pf1N1B4_1236 at 1407.067 kb on + strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.354 kb on + strand, within Pf1N1B4_1236 at 1407.430 kb on + strand at 1407.463 kb on - strand, within Pf1N1B4_1236 at 1407.546 kb on - strand, within Pf1N1B4_1236 at 1407.799 kb on - strand, within Pf1N1B4_1236 at 1408.006 kb on + strand, within Pf1N1B4_1236 at 1408.006 kb on + strand, within Pf1N1B4_1236 at 1408.006 kb on + strand at 1408.007 kb on - strand, within Pf1N1B4_1236 at 1408.007 kb on - strand, within Pf1N1B4_1236 at 1408.095 kb on + strand at 1408.096 kb on - strand at 1408.294 kb on + strand, within Pf1N1B4_1236 at 1408.299 kb on - strand, within Pf1N1B4_1236 at 1408.299 kb on - strand, within Pf1N1B4_1236 at 1408.299 kb on - strand at 1408.328 kb on + strand, within Pf1N1B4_1236 at 1408.328 kb on + strand, within Pf1N1B4_1236 at 1408.334 kb on - strand, within Pf1N1B4_1236 at 1408.442 kb on - strand, within Pf1N1B4_1236 at 1408.880 kb on + strand, within Pf1N1B4_1236 at 1408.880 kb on + strand, within Pf1N1B4_1236 at 1408.885 kb on - strand, within Pf1N1B4_1236 at 1408.885 kb on - strand, within Pf1N1B4_1236 at 1408.885 kb on - strand, within Pf1N1B4_1236 at 1409.167 kb on - strand at 1409.267 kb on + strand at 1409.267 kb on + strand at 1409.268 kb on - strand at 1409.268 kb on - strand at 1409.300 kb on + strand at 1409.301 kb on - strand at 1409.301 kb on - strand at 1409.301 kb on - strand at 1409.320 kb on + strand at 1409.330 kb on - strand at 1409.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 1,404,998 - Pf1N1B4_1235 0.77 -0.0 1,405,377 + Pf1N1B4_1235 0.87 -1.8 1,405,629 + -1.2 1,405,749 - +0.1 1,405,761 + +0.5 1,405,761 + -0.3 1,405,761 + +1.5 1,406,030 - +0.8 1,406,030 - -2.0 1,406,098 - -1.9 1,406,225 + Pf1N1B4_1236 0.11 -1.2 1,406,403 + Pf1N1B4_1236 0.16 -2.8 1,406,580 + Pf1N1B4_1236 0.21 -1.5 1,406,739 + Pf1N1B4_1236 0.26 -0.8 1,406,740 - Pf1N1B4_1236 0.26 -0.2 1,406,797 + Pf1N1B4_1236 0.27 +0.8 1,406,874 + Pf1N1B4_1236 0.30 -2.0 1,406,874 + Pf1N1B4_1236 0.30 -0.8 1,406,875 - Pf1N1B4_1236 0.30 -0.8 1,406,896 + Pf1N1B4_1236 0.30 -0.8 1,406,897 - Pf1N1B4_1236 0.30 +0.8 1,407,067 + Pf1N1B4_1236 0.35 -1.3 1,407,067 + Pf1N1B4_1236 0.35 +0.5 1,407,067 + Pf1N1B4_1236 0.35 -0.0 1,407,068 - Pf1N1B4_1236 0.35 -1.7 1,407,068 - Pf1N1B4_1236 0.35 -2.2 1,407,068 - Pf1N1B4_1236 0.35 -0.5 1,407,068 - Pf1N1B4_1236 0.35 -1.3 1,407,354 + Pf1N1B4_1236 0.44 +0.4 1,407,430 + +0.5 1,407,463 - Pf1N1B4_1236 0.47 +2.0 1,407,546 - Pf1N1B4_1236 0.49 -1.6 1,407,799 - Pf1N1B4_1236 0.57 +0.5 1,408,006 + Pf1N1B4_1236 0.62 -1.4 1,408,006 + Pf1N1B4_1236 0.62 -0.0 1,408,006 + +0.5 1,408,007 - Pf1N1B4_1236 0.63 -0.2 1,408,007 - Pf1N1B4_1236 0.63 -0.5 1,408,095 + +0.2 1,408,096 - +1.5 1,408,294 + Pf1N1B4_1236 0.71 -0.5 1,408,299 - Pf1N1B4_1236 0.71 -0.8 1,408,299 - Pf1N1B4_1236 0.71 +0.3 1,408,299 - -0.0 1,408,328 + Pf1N1B4_1236 0.72 +2.0 1,408,328 + Pf1N1B4_1236 0.72 -1.4 1,408,334 - Pf1N1B4_1236 0.72 +0.1 1,408,442 - Pf1N1B4_1236 0.75 +0.2 1,408,880 + Pf1N1B4_1236 0.88 -1.5 1,408,880 + Pf1N1B4_1236 0.88 -0.3 1,408,885 - Pf1N1B4_1236 0.88 -1.6 1,408,885 - Pf1N1B4_1236 0.88 +0.7 1,408,885 - Pf1N1B4_1236 0.88 -1.5 1,409,167 - -1.3 1,409,267 + -0.8 1,409,267 + +0.4 1,409,268 - -0.5 1,409,268 - +0.5 1,409,300 + -0.0 1,409,301 - -1.5 1,409,301 - -0.8 1,409,301 - -1.0 1,409,320 + +0.5 1,409,330 - +1.5 1,409,342 + -1.9
Or see this region's nucleotide sequence