Experiment: copper (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1176 and Pf1N1B4_1177 are separated by 102 nucleotides Pf1N1B4_1177 and Pf1N1B4_1178 are separated by 241 nucleotides Pf1N1B4_1178 and Pf1N1B4_1179 are separated by 75 nucleotides Pf1N1B4_1179 and Pf1N1B4_1180 are separated by 97 nucleotides
Pf1N1B4_1176 - Chaperone protein DnaK, at 1,326,921 to 1,328,837
_1176
Pf1N1B4_1177 - Heat shock protein GrpE, at 1,328,940 to 1,329,506
_1177
Pf1N1B4_1178 - DNA repair protein RecN, at 1,329,748 to 1,331,421
_1178
Pf1N1B4_1179 - Ferric uptake regulation protein FUR, at 1,331,497 to 1,331,901
_1179
Pf1N1B4_1180 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 1,331,999 to 1,332,526
_1180
Position (kb)
1329
1330
1331
1332 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1328.902 kb on - strand at 1328.917 kb on - strand at 1328.917 kb on - strand at 1329.560 kb on - strand at 1329.562 kb on - strand at 1329.591 kb on + strand at 1329.714 kb on - strand at 1329.747 kb on + strand at 1329.748 kb on - strand at 1329.748 kb on - strand at 1329.767 kb on + strand at 1329.767 kb on + strand at 1329.767 kb on + strand at 1329.768 kb on - strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.772 kb on - strand at 1329.772 kb on - strand at 1329.772 kb on - strand at 1329.772 kb on - strand at 1329.775 kb on + strand at 1329.775 kb on + strand at 1329.775 kb on + strand at 1330.192 kb on + strand, within Pf1N1B4_1178 at 1330.472 kb on + strand, within Pf1N1B4_1178 at 1330.472 kb on + strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.598 kb on + strand, within Pf1N1B4_1178 at 1330.645 kb on + strand, within Pf1N1B4_1178 at 1330.672 kb on + strand, within Pf1N1B4_1178 at 1330.673 kb on - strand, within Pf1N1B4_1178 at 1330.673 kb on - strand, within Pf1N1B4_1178 at 1330.673 kb on - strand, within Pf1N1B4_1178 at 1330.734 kb on - strand, within Pf1N1B4_1178 at 1330.734 kb on - strand, within Pf1N1B4_1178 at 1330.804 kb on + strand, within Pf1N1B4_1178 at 1330.804 kb on + strand, within Pf1N1B4_1178 at 1330.804 kb on + strand, within Pf1N1B4_1178 at 1330.816 kb on + strand, within Pf1N1B4_1178 at 1330.883 kb on + strand, within Pf1N1B4_1178 at 1330.883 kb on + strand, within Pf1N1B4_1178 at 1330.947 kb on + strand, within Pf1N1B4_1178 at 1330.947 kb on + strand, within Pf1N1B4_1178 at 1330.986 kb on - strand, within Pf1N1B4_1178 at 1331.039 kb on + strand, within Pf1N1B4_1178 at 1331.064 kb on + strand, within Pf1N1B4_1178 at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.265 kb on - strand at 1331.265 kb on - strand at 1331.295 kb on + strand at 1331.295 kb on + strand at 1331.296 kb on - strand at 1331.296 kb on - strand at 1331.419 kb on + strand at 1331.498 kb on + strand at 1331.499 kb on - strand at 1331.499 kb on - strand at 1332.373 kb on + strand, within Pf1N1B4_1180 at 1332.373 kb on + strand, within Pf1N1B4_1180
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 2.5 mM remove 1,328,902 - +0.5 1,328,917 - +1.0 1,328,917 - -0.3 1,329,560 - +0.9 1,329,562 - -0.9 1,329,591 + +1.8 1,329,714 - -1.1 1,329,747 + +0.2 1,329,748 - -0.8 1,329,748 - -1.8 1,329,767 + +0.4 1,329,767 + +0.5 1,329,767 + +1.1 1,329,768 - +1.5 1,329,771 + +0.5 1,329,771 + +2.1 1,329,771 + -0.6 1,329,771 + -0.1 1,329,771 + -1.1 1,329,771 + -0.4 1,329,771 + +0.7 1,329,772 - +0.5 1,329,772 - +2.1 1,329,772 - +0.1 1,329,772 - +0.9 1,329,775 + -1.9 1,329,775 + +0.9 1,329,775 + +0.5 1,330,192 + Pf1N1B4_1178 0.27 -0.9 1,330,472 + Pf1N1B4_1178 0.43 +0.5 1,330,472 + Pf1N1B4_1178 0.43 +1.1 1,330,473 - Pf1N1B4_1178 0.43 -1.1 1,330,473 - Pf1N1B4_1178 0.43 -1.1 1,330,473 - Pf1N1B4_1178 0.43 -1.3 1,330,473 - Pf1N1B4_1178 0.43 -0.1 1,330,598 + Pf1N1B4_1178 0.51 +1.2 1,330,645 + Pf1N1B4_1178 0.54 -1.2 1,330,672 + Pf1N1B4_1178 0.55 -1.1 1,330,673 - Pf1N1B4_1178 0.55 +1.5 1,330,673 - Pf1N1B4_1178 0.55 -0.1 1,330,673 - Pf1N1B4_1178 0.55 -1.7 1,330,734 - Pf1N1B4_1178 0.59 -0.8 1,330,734 - Pf1N1B4_1178 0.59 -2.1 1,330,804 + Pf1N1B4_1178 0.63 -0.6 1,330,804 + Pf1N1B4_1178 0.63 -0.8 1,330,804 + Pf1N1B4_1178 0.63 +0.2 1,330,816 + Pf1N1B4_1178 0.64 -0.2 1,330,883 + Pf1N1B4_1178 0.68 -0.0 1,330,883 + Pf1N1B4_1178 0.68 -0.5 1,330,947 + Pf1N1B4_1178 0.72 -1.1 1,330,947 + Pf1N1B4_1178 0.72 -0.2 1,330,986 - Pf1N1B4_1178 0.74 -1.1 1,331,039 + Pf1N1B4_1178 0.77 -0.5 1,331,064 + Pf1N1B4_1178 0.79 -1.2 1,331,264 + -0.6 1,331,264 + -1.6 1,331,264 + +0.0 1,331,264 + +0.2 1,331,264 + +0.8 1,331,265 - -0.9 1,331,265 - +1.1 1,331,295 + -0.1 1,331,295 + -1.1 1,331,296 - +1.2 1,331,296 - +1.1 1,331,419 + +0.7 1,331,498 + -1.2 1,331,499 - +0.2 1,331,499 - -0.5 1,332,373 + Pf1N1B4_1180 0.71 -1.3 1,332,373 + Pf1N1B4_1180 0.71 -0.1
Or see this region's nucleotide sequence