Experiment: R2A with copper (II) chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22215 and CA265_RS22220 are separated by 144 nucleotides CA265_RS22220 and CA265_RS22225 are separated by 105 nucleotides CA265_RS22225 and CA265_RS22230 are separated by 1 nucleotides CA265_RS22230 and CA265_RS22235 are separated by 12 nucleotides
CA265_RS22215: CA265_RS22215 - type VI secretion system needle protein Hcp, at 5,459,673 to 5,460,068
_RS22215
CA265_RS22220: CA265_RS22220 - hypothetical protein, at 5,460,213 to 5,460,608
_RS22220
CA265_RS22225: CA265_RS22225 - hypothetical protein, at 5,460,714 to 5,461,265
_RS22225
CA265_RS22230: CA265_RS22230 - hypothetical protein, at 5,461,267 to 5,461,854
_RS22230
CA265_RS22235: CA265_RS22235 - type IV secretion protein Rhs, at 5,461,867 to 5,463,666
_RS22235
Position (kb) 5460
5461
5462 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5460.047 kb on + strand at 5460.084 kb on - strand at 5460.099 kb on - strand at 5460.123 kb on - strand at 5460.123 kb on - strand at 5460.124 kb on + strand at 5460.125 kb on - strand at 5460.125 kb on - strand at 5460.133 kb on + strand at 5460.134 kb on - strand at 5460.134 kb on - strand at 5460.138 kb on + strand at 5460.204 kb on - strand at 5460.210 kb on - strand at 5460.463 kb on + strand, within CA265_RS22220 at 5460.481 kb on - strand, within CA265_RS22220 at 5460.631 kb on - strand at 5460.638 kb on + strand at 5460.639 kb on - strand at 5460.694 kb on - strand at 5460.694 kb on - strand at 5460.724 kb on + strand at 5460.777 kb on + strand, within CA265_RS22225 at 5460.777 kb on + strand, within CA265_RS22225 at 5460.777 kb on + strand, within CA265_RS22225 at 5460.777 kb on + strand, within CA265_RS22225 at 5460.778 kb on - strand, within CA265_RS22225 at 5460.779 kb on + strand, within CA265_RS22225 at 5460.788 kb on - strand, within CA265_RS22225 at 5460.857 kb on + strand, within CA265_RS22225 at 5460.897 kb on - strand, within CA265_RS22225 at 5460.986 kb on + strand, within CA265_RS22225 at 5461.086 kb on + strand, within CA265_RS22225 at 5461.087 kb on - strand, within CA265_RS22225 at 5461.224 kb on - strand at 5461.224 kb on - strand at 5461.224 kb on - strand at 5461.232 kb on + strand at 5461.233 kb on - strand at 5461.323 kb on + strand at 5461.369 kb on + strand, within CA265_RS22230 at 5461.374 kb on + strand, within CA265_RS22230 at 5461.375 kb on - strand, within CA265_RS22230 at 5461.375 kb on - strand, within CA265_RS22230 at 5461.380 kb on + strand, within CA265_RS22230 at 5461.425 kb on + strand, within CA265_RS22230 at 5461.426 kb on - strand, within CA265_RS22230 at 5461.426 kb on - strand, within CA265_RS22230 at 5461.428 kb on + strand, within CA265_RS22230 at 5461.429 kb on - strand, within CA265_RS22230 at 5461.540 kb on - strand, within CA265_RS22230 at 5461.555 kb on - strand, within CA265_RS22230 at 5461.620 kb on + strand, within CA265_RS22230 at 5461.620 kb on + strand, within CA265_RS22230 at 5461.738 kb on - strand, within CA265_RS22230 at 5461.738 kb on - strand, within CA265_RS22230 at 5461.788 kb on + strand, within CA265_RS22230 at 5461.789 kb on - strand, within CA265_RS22230 at 5461.789 kb on - strand, within CA265_RS22230 at 5461.894 kb on - strand at 5461.894 kb on - strand at 5461.898 kb on + strand at 5461.899 kb on - strand at 5461.933 kb on - strand at 5461.933 kb on - strand at 5461.937 kb on + strand at 5461.951 kb on - strand at 5461.964 kb on - strand at 5461.981 kb on - strand at 5462.164 kb on + strand, within CA265_RS22235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.16 mM remove 5,460,047 + +1.9 5,460,084 - -1.2 5,460,099 - +0.3 5,460,123 - +0.7 5,460,123 - +1.3 5,460,124 + +1.7 5,460,125 - +1.7 5,460,125 - +2.1 5,460,133 + +0.1 5,460,134 - +0.4 5,460,134 - -1.1 5,460,138 + -3.0 5,460,204 - -1.4 5,460,210 - +0.6 5,460,463 + CA265_RS22220 0.63 -0.0 5,460,481 - CA265_RS22220 0.68 -2.2 5,460,631 - +1.5 5,460,638 + -2.1 5,460,639 - +1.3 5,460,694 - +2.6 5,460,694 - -2.7 5,460,724 + -1.3 5,460,777 + CA265_RS22225 0.11 -2.1 5,460,777 + CA265_RS22225 0.11 +1.4 5,460,777 + CA265_RS22225 0.11 +0.7 5,460,777 + CA265_RS22225 0.11 -1.7 5,460,778 - CA265_RS22225 0.12 -1.0 5,460,779 + CA265_RS22225 0.12 -2.0 5,460,788 - CA265_RS22225 0.13 -1.7 5,460,857 + CA265_RS22225 0.26 -0.6 5,460,897 - CA265_RS22225 0.33 +0.3 5,460,986 + CA265_RS22225 0.49 +0.5 5,461,086 + CA265_RS22225 0.67 -0.9 5,461,087 - CA265_RS22225 0.68 -0.9 5,461,224 - +0.4 5,461,224 - -0.6 5,461,224 - -0.8 5,461,232 + -0.3 5,461,233 - -0.2 5,461,323 + +0.5 5,461,369 + CA265_RS22230 0.17 -3.4 5,461,374 + CA265_RS22230 0.18 +0.0 5,461,375 - CA265_RS22230 0.18 -1.1 5,461,375 - CA265_RS22230 0.18 +0.7 5,461,380 + CA265_RS22230 0.19 +0.7 5,461,425 + CA265_RS22230 0.27 -0.1 5,461,426 - CA265_RS22230 0.27 -0.9 5,461,426 - CA265_RS22230 0.27 -0.1 5,461,428 + CA265_RS22230 0.27 -3.9 5,461,429 - CA265_RS22230 0.28 -2.4 5,461,540 - CA265_RS22230 0.46 +0.5 5,461,555 - CA265_RS22230 0.49 -2.5 5,461,620 + CA265_RS22230 0.60 +1.6 5,461,620 + CA265_RS22230 0.60 -3.5 5,461,738 - CA265_RS22230 0.80 -2.7 5,461,738 - CA265_RS22230 0.80 -0.5 5,461,788 + CA265_RS22230 0.89 -1.3 5,461,789 - CA265_RS22230 0.89 -1.7 5,461,789 - CA265_RS22230 0.89 -0.7 5,461,894 - -1.9 5,461,894 - +0.2 5,461,898 + -0.2 5,461,899 - -1.8 5,461,933 - +0.3 5,461,933 - +0.7 5,461,937 + -1.0 5,461,951 - -3.8 5,461,964 - -0.4 5,461,981 - -0.2 5,462,164 + CA265_RS22235 0.17 -1.8
Or see this region's nucleotide sequence