Experiment: R2A with copper (II) chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10585 and CA265_RS10590 are separated by 198 nucleotides CA265_RS10590 and CA265_RS10595 are separated by 88 nucleotides CA265_RS10595 and CA265_RS10600 are separated by 160 nucleotides CA265_RS10600 and CA265_RS10605 overlap by 14 nucleotides
CA265_RS10585: CA265_RS10585 - transcriptional regulator, at 2,619,819 to 2,620,181
_RS10585
CA265_RS10590: CA265_RS10590 - hypothetical protein, at 2,620,380 to 2,620,880
_RS10590
CA265_RS10595: CA265_RS10595 - dicarboxylate/amino acid:cation symporter, at 2,620,969 to 2,622,345
_RS10595
CA265_RS10600: CA265_RS10600 - RNA polymerase subunit sigma-24, at 2,622,506 to 2,623,003
_RS10600
CA265_RS10605: CA265_RS10605 - hypothetical protein, at 2,622,990 to 2,623,589
_RS10605
Position (kb)
2620
2621
2622
2623 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2619.991 kb on + strand, within CA265_RS10585 at 2620.056 kb on - strand, within CA265_RS10585 at 2620.068 kb on - strand, within CA265_RS10585 at 2620.095 kb on + strand, within CA265_RS10585 at 2620.103 kb on + strand, within CA265_RS10585 at 2620.115 kb on + strand, within CA265_RS10585 at 2620.124 kb on + strand, within CA265_RS10585 at 2620.124 kb on + strand, within CA265_RS10585 at 2620.128 kb on + strand, within CA265_RS10585 at 2620.259 kb on + strand at 2620.260 kb on - strand at 2620.294 kb on - strand at 2620.747 kb on + strand, within CA265_RS10590 at 2620.811 kb on + strand at 2621.002 kb on + strand at 2621.002 kb on + strand at 2621.227 kb on + strand, within CA265_RS10595 at 2621.227 kb on + strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.228 kb on - strand, within CA265_RS10595 at 2621.288 kb on - strand, within CA265_RS10595 at 2621.353 kb on + strand, within CA265_RS10595 at 2621.354 kb on - strand, within CA265_RS10595 at 2621.398 kb on - strand, within CA265_RS10595 at 2621.530 kb on + strand, within CA265_RS10595 at 2621.532 kb on + strand, within CA265_RS10595 at 2621.533 kb on - strand, within CA265_RS10595 at 2621.538 kb on - strand, within CA265_RS10595 at 2621.546 kb on - strand, within CA265_RS10595 at 2621.549 kb on - strand, within CA265_RS10595 at 2621.555 kb on - strand, within CA265_RS10595 at 2621.621 kb on + strand, within CA265_RS10595 at 2621.622 kb on - strand, within CA265_RS10595 at 2621.684 kb on - strand, within CA265_RS10595 at 2621.855 kb on - strand, within CA265_RS10595 at 2621.979 kb on + strand, within CA265_RS10595 at 2622.049 kb on + strand, within CA265_RS10595 at 2622.163 kb on - strand, within CA265_RS10595 at 2622.239 kb on - strand at 2622.239 kb on - strand at 2622.241 kb on - strand at 2622.385 kb on - strand at 2622.390 kb on - strand at 2622.482 kb on + strand at 2622.493 kb on + strand at 2622.493 kb on + strand at 2622.495 kb on + strand at 2622.495 kb on + strand at 2622.525 kb on + strand at 2622.525 kb on + strand at 2622.570 kb on + strand, within CA265_RS10600 at 2622.581 kb on + strand, within CA265_RS10600 at 2622.583 kb on + strand, within CA265_RS10600 at 2622.715 kb on - strand, within CA265_RS10600 at 2622.719 kb on + strand, within CA265_RS10600 at 2622.719 kb on + strand, within CA265_RS10600 at 2622.756 kb on + strand, within CA265_RS10600 at 2622.763 kb on + strand, within CA265_RS10600 at 2622.783 kb on + strand, within CA265_RS10600 at 2622.826 kb on + strand, within CA265_RS10600 at 2622.869 kb on - strand, within CA265_RS10600 at 2622.893 kb on + strand, within CA265_RS10600 at 2622.959 kb on - strand at 2622.959 kb on - strand at 2622.978 kb on + strand at 2622.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with copper (II) chloride 0.16 mM remove 2,619,991 + CA265_RS10585 0.47 -0.1 2,620,056 - CA265_RS10585 0.65 +0.6 2,620,068 - CA265_RS10585 0.69 +0.1 2,620,095 + CA265_RS10585 0.76 +0.6 2,620,103 + CA265_RS10585 0.78 -0.3 2,620,115 + CA265_RS10585 0.82 +0.1 2,620,124 + CA265_RS10585 0.84 -2.0 2,620,124 + CA265_RS10585 0.84 -1.7 2,620,128 + CA265_RS10585 0.85 +0.1 2,620,259 + +0.6 2,620,260 - +1.3 2,620,294 - -1.6 2,620,747 + CA265_RS10590 0.73 +1.9 2,620,811 + -0.3 2,621,002 + +2.4 2,621,002 + +0.5 2,621,227 + CA265_RS10595 0.19 +0.5 2,621,227 + CA265_RS10595 0.19 +1.5 2,621,228 - CA265_RS10595 0.19 +0.7 2,621,228 - CA265_RS10595 0.19 -2.2 2,621,228 - CA265_RS10595 0.19 -2.5 2,621,228 - CA265_RS10595 0.19 +2.6 2,621,288 - CA265_RS10595 0.23 -0.7 2,621,353 + CA265_RS10595 0.28 -0.3 2,621,354 - CA265_RS10595 0.28 +0.7 2,621,398 - CA265_RS10595 0.31 +1.0 2,621,530 + CA265_RS10595 0.41 +1.8 2,621,532 + CA265_RS10595 0.41 -1.9 2,621,533 - CA265_RS10595 0.41 +2.1 2,621,538 - CA265_RS10595 0.41 +1.0 2,621,546 - CA265_RS10595 0.42 +1.7 2,621,549 - CA265_RS10595 0.42 +0.1 2,621,555 - CA265_RS10595 0.43 +1.6 2,621,621 + CA265_RS10595 0.47 +0.5 2,621,622 - CA265_RS10595 0.47 +1.3 2,621,684 - CA265_RS10595 0.52 +1.3 2,621,855 - CA265_RS10595 0.64 +1.1 2,621,979 + CA265_RS10595 0.73 +0.1 2,622,049 + CA265_RS10595 0.78 +1.5 2,622,163 - CA265_RS10595 0.87 +2.3 2,622,239 - -1.2 2,622,239 - +0.2 2,622,241 - +0.8 2,622,385 - -1.4 2,622,390 - +0.7 2,622,482 + -1.6 2,622,493 + -0.1 2,622,493 + -1.4 2,622,495 + +0.7 2,622,495 + -1.1 2,622,525 + +0.6 2,622,525 + -1.5 2,622,570 + CA265_RS10600 0.13 +1.7 2,622,581 + CA265_RS10600 0.15 -1.5 2,622,583 + CA265_RS10600 0.15 +0.7 2,622,715 - CA265_RS10600 0.42 -1.3 2,622,719 + CA265_RS10600 0.43 -0.7 2,622,719 + CA265_RS10600 0.43 -1.0 2,622,756 + CA265_RS10600 0.50 -0.5 2,622,763 + CA265_RS10600 0.52 -0.6 2,622,783 + CA265_RS10600 0.56 -2.8 2,622,826 + CA265_RS10600 0.64 -3.1 2,622,869 - CA265_RS10600 0.73 -0.4 2,622,893 + CA265_RS10600 0.78 -0.2 2,622,959 - +0.0 2,622,959 - -0.6 2,622,978 + +3.5 2,622,979 - -3.0
Or see this region's nucleotide sequence