Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01885 and SMc01884 are separated by 187 nucleotides SMc01884 and SMc01883 are separated by 116 nucleotides SMc01883 and SMc01882 are separated by 3 nucleotides SMc01882 and SMc01881 are separated by 204 nucleotides
SMc01885: SMc01885 - aminopeptidase P protein, at 2,321,566 to 2,323,401
SMc01885
SMc01884: SMc01884 - signal peptide protein, at 2,323,589 to 2,323,828
SMc01884
SMc01883: SMc01883 - hypothetical protein, at 2,323,945 to 2,324,253
SMc01883
SMc01882: SMc01882 - hypothetical protein, at 2,324,257 to 2,324,973
SMc01882
SMc01881: SMc01881 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 2,325,178 to 2,325,999
SMc01881
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2323.033 kb on + strand, within SMc01885 at 2323.034 kb on - strand, within SMc01885 at 2323.034 kb on - strand, within SMc01885 at 2323.123 kb on + strand, within SMc01885 at 2323.124 kb on - strand, within SMc01885 at 2323.210 kb on - strand, within SMc01885 at 2323.306 kb on + strand at 2323.380 kb on + strand at 2323.381 kb on - strand at 2323.400 kb on - strand at 2323.494 kb on - strand at 2323.495 kb on + strand at 2323.496 kb on - strand at 2323.883 kb on + strand at 2323.946 kb on + strand at 2323.946 kb on + strand at 2323.947 kb on - strand at 2324.069 kb on + strand, within SMc01883 at 2324.070 kb on - strand, within SMc01883 at 2324.070 kb on - strand, within SMc01883 at 2324.146 kb on + strand, within SMc01883 at 2324.146 kb on + strand, within SMc01883 at 2324.147 kb on - strand, within SMc01883 at 2324.166 kb on + strand, within SMc01883 at 2324.195 kb on + strand, within SMc01883 at 2324.195 kb on + strand, within SMc01883 at 2324.195 kb on + strand, within SMc01883 at 2324.195 kb on + strand, within SMc01883 at 2324.196 kb on - strand, within SMc01883 at 2324.225 kb on + strand at 2324.225 kb on + strand at 2324.225 kb on + strand at 2324.225 kb on + strand at 2324.226 kb on - strand at 2324.466 kb on - strand, within SMc01882 at 2324.541 kb on + strand, within SMc01882 at 2324.561 kb on + strand, within SMc01882 at 2324.561 kb on + strand, within SMc01882 at 2324.561 kb on + strand, within SMc01882 at 2324.561 kb on + strand, within SMc01882 at 2324.562 kb on - strand, within SMc01882 at 2324.585 kb on + strand, within SMc01882 at 2324.829 kb on - strand, within SMc01882 at 2324.849 kb on - strand, within SMc01882 at 2324.849 kb on - strand, within SMc01882 at 2325.018 kb on + strand at 2325.019 kb on - strand at 2325.134 kb on + strand at 2325.139 kb on + strand at 2325.139 kb on + strand at 2325.139 kb on + strand at 2325.140 kb on - strand at 2325.140 kb on - strand at 2325.140 kb on - strand at 2325.205 kb on + strand at 2325.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,323,033 + SMc01885 0.80 -4.5 2,323,034 - SMc01885 0.80 -2.6 2,323,034 - SMc01885 0.80 -0.4 2,323,123 + SMc01885 0.85 -2.3 2,323,124 - SMc01885 0.85 -2.9 2,323,210 - SMc01885 0.90 -1.4 2,323,306 + -1.8 2,323,380 + -3.0 2,323,381 - -1.5 2,323,400 - -0.3 2,323,494 - -0.0 2,323,495 + +0.8 2,323,496 - +0.1 2,323,883 + +1.4 2,323,946 + +0.4 2,323,946 + +0.4 2,323,947 - -2.6 2,324,069 + SMc01883 0.40 -0.3 2,324,070 - SMc01883 0.40 -0.7 2,324,070 - SMc01883 0.40 -0.2 2,324,146 + SMc01883 0.65 -1.3 2,324,146 + SMc01883 0.65 -0.6 2,324,147 - SMc01883 0.65 -0.6 2,324,166 + SMc01883 0.72 +0.0 2,324,195 + SMc01883 0.81 -0.1 2,324,195 + SMc01883 0.81 -0.7 2,324,195 + SMc01883 0.81 -0.3 2,324,195 + SMc01883 0.81 -0.7 2,324,196 - SMc01883 0.81 +0.8 2,324,225 + -0.3 2,324,225 + -0.1 2,324,225 + +1.4 2,324,225 + -0.1 2,324,226 - -2.1 2,324,466 - SMc01882 0.29 -0.2 2,324,541 + SMc01882 0.40 -0.4 2,324,561 + SMc01882 0.42 -1.0 2,324,561 + SMc01882 0.42 -1.8 2,324,561 + SMc01882 0.42 -0.7 2,324,561 + SMc01882 0.42 -0.7 2,324,562 - SMc01882 0.43 -1.3 2,324,585 + SMc01882 0.46 -0.5 2,324,829 - SMc01882 0.80 -0.5 2,324,849 - SMc01882 0.83 -1.7 2,324,849 - SMc01882 0.83 +0.2 2,325,018 + -0.1 2,325,019 - +0.7 2,325,134 + -0.5 2,325,139 + -0.6 2,325,139 + -0.3 2,325,139 + +1.2 2,325,140 - -1.0 2,325,140 - +1.3 2,325,140 - -0.0 2,325,205 + -2.2 2,325,206 - -1.4
Or see this region's nucleotide sequence