Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01021 and SMc01022 overlap by 4 nucleotides SMc01022 and SMc01023 are separated by 258 nucleotides SMc01023 and SMc01024 are separated by 122 nucleotides SMc01024 and SMc01025 are separated by 143 nucleotides
SMc01021: SMc01021 - hypothetical protein, at 1,546,790 to 1,547,395
SMc01021
SMc01022: SMc01022 - cytochrome B transmembrane protein, at 1,547,392 to 1,548,018
SMc01022
SMc01023: SMc01023 - triosephosphate isomerase, at 1,548,277 to 1,549,047
SMc01023
SMc01024: SMc01024 - preprotein translocase subunit SecG, at 1,549,170 to 1,549,655
SMc01024
SMc01025: SMc01025 - CTP synthetase, at 1,549,799 to 1,551,427
SMc01025
Position (kb)
1548
1549
1550 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1547.395 kb on + strand at 1547.395 kb on + strand at 1547.396 kb on - strand at 1547.396 kb on - strand at 1547.519 kb on + strand, within SMc01022 at 1547.519 kb on + strand, within SMc01022 at 1547.519 kb on + strand, within SMc01022 at 1547.519 kb on + strand, within SMc01022 at 1547.519 kb on + strand at 1547.520 kb on - strand, within SMc01022 at 1547.520 kb on - strand, within SMc01022 at 1547.520 kb on - strand, within SMc01022 at 1547.520 kb on - strand, within SMc01022 at 1547.520 kb on - strand, within SMc01022 at 1547.565 kb on - strand, within SMc01022 at 1547.565 kb on - strand, within SMc01022 at 1547.565 kb on - strand, within SMc01022 at 1547.565 kb on - strand, within SMc01022 at 1547.565 kb on - strand, within SMc01022 at 1547.654 kb on + strand, within SMc01022 at 1547.655 kb on - strand, within SMc01022 at 1547.876 kb on + strand, within SMc01022 at 1547.877 kb on - strand, within SMc01022 at 1548.048 kb on + strand at 1548.058 kb on + strand at 1548.059 kb on - strand at 1548.059 kb on - strand at 1548.073 kb on + strand at 1548.160 kb on + strand at 1548.217 kb on - strand at 1548.431 kb on - strand, within SMc01023 at 1548.431 kb on - strand, within SMc01023 at 1548.908 kb on - strand, within SMc01023 at 1549.018 kb on + strand at 1549.018 kb on + strand at 1549.019 kb on - strand at 1549.027 kb on + strand at 1549.027 kb on + strand at 1549.028 kb on - strand at 1549.028 kb on - strand at 1549.066 kb on + strand at 1549.444 kb on + strand, within SMc01024 at 1549.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,547,395 + -0.4 1,547,395 + +0.2 1,547,396 - +0.0 1,547,396 - +0.5 1,547,519 + SMc01022 0.20 +0.4 1,547,519 + SMc01022 0.20 +0.5 1,547,519 + SMc01022 0.20 -0.5 1,547,519 + SMc01022 0.20 -3.0 1,547,519 + +2.8 1,547,520 - SMc01022 0.20 -1.3 1,547,520 - SMc01022 0.20 +0.4 1,547,520 - SMc01022 0.20 +0.8 1,547,520 - SMc01022 0.20 -1.4 1,547,520 - SMc01022 0.20 +0.5 1,547,565 - SMc01022 0.28 +0.7 1,547,565 - SMc01022 0.28 -0.9 1,547,565 - SMc01022 0.28 -0.1 1,547,565 - SMc01022 0.28 -0.5 1,547,565 - SMc01022 0.28 -0.0 1,547,654 + SMc01022 0.42 -1.0 1,547,655 - SMc01022 0.42 -2.9 1,547,876 + SMc01022 0.77 -0.4 1,547,877 - SMc01022 0.77 -0.9 1,548,048 + -2.4 1,548,058 + +1.7 1,548,059 - -1.2 1,548,059 - +0.3 1,548,073 + -0.4 1,548,160 + -3.0 1,548,217 - -2.1 1,548,431 - SMc01023 0.20 +0.8 1,548,431 - SMc01023 0.20 +0.5 1,548,908 - SMc01023 0.82 -3.9 1,549,018 + +0.0 1,549,018 + -0.2 1,549,019 - +0.4 1,549,027 + -0.9 1,549,027 + +0.2 1,549,028 - +0.4 1,549,028 - +0.7 1,549,066 + +1.1 1,549,444 + SMc01024 0.56 +0.9 1,549,625 + -0.1
Or see this region's nucleotide sequence