Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1973 and SMa1974 overlap by 1 nucleotides SMa1974 and SMa1976 are separated by 180 nucleotides SMa1976 and SMa1978 are separated by 26 nucleotides SMa1978 and SMa1979 are separated by 90 nucleotides
SMa1973: SMa1973 - hypothetical protein, at 1,122,124 to 1,122,483
SMa1973
SMa1974: SMa1974 - hypothetical protein, at 1,122,483 to 1,123,199
SMa1974
SMa1976: SMa1976 - hypothetical protein, at 1,123,380 to 1,124,171
SMa1976
SMa1978: SMa1978 - hydrolase, at 1,124,198 to 1,124,902
SMa1978
SMa1979: SMa1979 - LysR family transcriptional regulator, at 1,124,993 to 1,125,865
SMa1979
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1122.442 kb on - strand, within SMa1973 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.583 kb on - strand, within SMa1974 at 1122.688 kb on - strand, within SMa1974 at 1122.818 kb on + strand, within SMa1974 at 1122.921 kb on - strand, within SMa1974 at 1122.921 kb on - strand, within SMa1974 at 1123.177 kb on + strand at 1123.178 kb on - strand at 1123.178 kb on - strand at 1123.182 kb on - strand at 1123.182 kb on - strand at 1123.218 kb on - strand at 1123.316 kb on + strand at 1123.338 kb on + strand at 1123.390 kb on + strand at 1123.391 kb on - strand at 1123.567 kb on + strand, within SMa1976 at 1123.567 kb on + strand, within SMa1976 at 1123.570 kb on + strand, within SMa1976 at 1123.570 kb on + strand, within SMa1976 at 1123.640 kb on + strand, within SMa1976 at 1123.641 kb on - strand, within SMa1976 at 1123.705 kb on + strand, within SMa1976 at 1123.705 kb on + strand, within SMa1976 at 1123.706 kb on - strand, within SMa1976 at 1123.808 kb on + strand, within SMa1976 at 1123.809 kb on - strand, within SMa1976 at 1123.809 kb on - strand, within SMa1976 at 1123.809 kb on - strand, within SMa1976 at 1123.815 kb on - strand, within SMa1976 at 1124.156 kb on + strand at 1124.175 kb on + strand at 1124.175 kb on + strand at 1124.175 kb on + strand at 1124.176 kb on - strand at 1124.176 kb on - strand at 1124.176 kb on - strand at 1124.176 kb on - strand at 1124.177 kb on + strand at 1124.177 kb on + strand at 1124.178 kb on - strand at 1124.199 kb on + strand at 1124.272 kb on - strand, within SMa1978 at 1124.349 kb on + strand, within SMa1978 at 1124.424 kb on - strand, within SMa1978 at 1124.424 kb on - strand, within SMa1978 at 1124.442 kb on + strand, within SMa1978 at 1124.442 kb on + strand, within SMa1978 at 1124.443 kb on - strand, within SMa1978 at 1124.536 kb on + strand, within SMa1978 at 1124.573 kb on - strand, within SMa1978 at 1124.573 kb on - strand, within SMa1978 at 1124.611 kb on + strand, within SMa1978 at 1124.648 kb on + strand, within SMa1978 at 1124.649 kb on - strand, within SMa1978 at 1124.754 kb on + strand, within SMa1978 at 1124.755 kb on - strand, within SMa1978 at 1124.765 kb on - strand, within SMa1978 at 1124.856 kb on + strand at 1124.938 kb on + strand at 1125.058 kb on + strand at 1125.059 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,122,442 - SMa1973 0.88 +0.1 1,122,575 + SMa1974 0.13 -0.9 1,122,575 + SMa1974 0.13 +0.2 1,122,575 + SMa1974 0.13 -0.6 1,122,575 + SMa1974 0.13 -1.2 1,122,575 + SMa1974 0.13 +0.6 1,122,576 - SMa1974 0.13 +0.6 1,122,576 - SMa1974 0.13 +0.1 1,122,576 - SMa1974 0.13 +0.2 1,122,576 - SMa1974 0.13 -0.7 1,122,583 - SMa1974 0.14 +0.4 1,122,688 - SMa1974 0.29 -1.2 1,122,818 + SMa1974 0.47 -0.3 1,122,921 - SMa1974 0.61 -0.7 1,122,921 - SMa1974 0.61 -0.0 1,123,177 + -0.3 1,123,178 - +0.6 1,123,178 - -1.0 1,123,182 - +0.6 1,123,182 - -0.7 1,123,218 - -0.1 1,123,316 + +0.4 1,123,338 + -1.3 1,123,390 + +0.3 1,123,391 - +0.9 1,123,567 + SMa1976 0.24 +0.2 1,123,567 + SMa1976 0.24 -1.5 1,123,570 + SMa1976 0.24 +0.4 1,123,570 + SMa1976 0.24 -2.1 1,123,640 + SMa1976 0.33 +2.9 1,123,641 - SMa1976 0.33 +0.3 1,123,705 + SMa1976 0.41 -1.7 1,123,705 + SMa1976 0.41 +0.6 1,123,706 - SMa1976 0.41 -0.4 1,123,808 + SMa1976 0.54 -0.5 1,123,809 - SMa1976 0.54 -0.8 1,123,809 - SMa1976 0.54 -2.8 1,123,809 - SMa1976 0.54 +0.6 1,123,815 - SMa1976 0.55 -1.5 1,124,156 + +0.2 1,124,175 + -1.5 1,124,175 + -0.5 1,124,175 + +0.7 1,124,176 - +0.7 1,124,176 - +1.6 1,124,176 - -1.0 1,124,176 - -1.2 1,124,177 + +1.2 1,124,177 + +0.4 1,124,178 - -0.4 1,124,199 + -0.0 1,124,272 - SMa1978 0.10 -0.4 1,124,349 + SMa1978 0.21 +0.3 1,124,424 - SMa1978 0.32 +0.0 1,124,424 - SMa1978 0.32 -0.3 1,124,442 + SMa1978 0.35 -0.5 1,124,442 + SMa1978 0.35 +1.1 1,124,443 - SMa1978 0.35 -0.3 1,124,536 + SMa1978 0.48 +0.8 1,124,573 - SMa1978 0.53 -0.1 1,124,573 - SMa1978 0.53 -0.0 1,124,611 + SMa1978 0.59 +0.0 1,124,648 + SMa1978 0.64 -0.8 1,124,649 - SMa1978 0.64 +0.4 1,124,754 + SMa1978 0.79 -0.5 1,124,755 - SMa1978 0.79 +1.4 1,124,765 - SMa1978 0.80 -0.9 1,124,856 + -0.7 1,124,938 + -2.3 1,125,058 + +0.5 1,125,059 - +0.3
Or see this region's nucleotide sequence