Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1568 and SMa1570 are separated by 43 nucleotides SMa1570 and SMa1572 are separated by 344 nucleotides SMa1572 and SMa1573 overlap by 4 nucleotides
SMa1568: SMa1568 - CpaF2 pilus assembly protein, at 867,356 to 868,849
SMa1568
SMa1570: SMa1570 - PilA2 pilus assembly protein, at 868,893 to 869,063
SMa1570
SMa1572: SMa1572 - hypothetical protein, at 869,408 to 869,833
SMa1572
SMa1573: SMa1573 - CpaE2 pilus assembly protein, at 869,830 to 871,590
SMa1573
Position (kb) 869
870 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 868.523 kb on + strand, within SMa1568 at 868.524 kb on - strand, within SMa1568 at 868.632 kb on + strand, within SMa1568 at 868.633 kb on - strand, within SMa1568 at 868.950 kb on + strand, within SMa1570 at 868.950 kb on + strand, within SMa1570 at 868.951 kb on - strand, within SMa1570 at 869.165 kb on + strand at 869.166 kb on - strand at 869.166 kb on - strand at 869.168 kb on - strand at 869.335 kb on + strand at 869.336 kb on - strand at 869.487 kb on + strand, within SMa1572 at 869.487 kb on + strand, within SMa1572 at 869.514 kb on - strand, within SMa1572 at 869.701 kb on - strand, within SMa1572 at 869.701 kb on - strand, within SMa1572 at 869.701 kb on - strand, within SMa1572 at 869.930 kb on + strand at 869.931 kb on - strand at 870.233 kb on - strand, within SMa1573 at 870.233 kb on - strand, within SMa1573 at 870.413 kb on - strand, within SMa1573 at 870.466 kb on - strand, within SMa1573 at 870.706 kb on + strand, within SMa1573
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 868,523 + SMa1568 0.78 +2.0 868,524 - SMa1568 0.78 +0.6 868,632 + SMa1568 0.85 -0.1 868,633 - SMa1568 0.85 +0.9 868,950 + SMa1570 0.33 -2.9 868,950 + SMa1570 0.33 +0.9 868,951 - SMa1570 0.34 +0.1 869,165 + +0.5 869,166 - -1.0 869,166 - -0.1 869,168 - +0.9 869,335 + -1.5 869,336 - -0.5 869,487 + SMa1572 0.19 +0.4 869,487 + SMa1572 0.19 +0.1 869,514 - SMa1572 0.25 -1.2 869,701 - SMa1572 0.69 -0.8 869,701 - SMa1572 0.69 -1.7 869,701 - SMa1572 0.69 -1.0 869,930 + -0.1 869,931 - +1.1 870,233 - SMa1573 0.23 -0.4 870,233 - SMa1573 0.23 -0.8 870,413 - SMa1573 0.33 -0.4 870,466 - SMa1573 0.36 +0.5 870,706 + SMa1573 0.50 -2.2
Or see this region's nucleotide sequence