Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa5020 and SMa0421 overlap by 13 nucleotides SMa0421 and SMa5021 overlap by 4 nucleotides SMa5021 and SMa0424 are separated by 34 nucleotides SMa0424 and SMa0426 overlap by 4 nucleotides
SMa5020: SMa5020 - hypothetical protein, at 227,425 to 228,318
SMa5020
SMa0421: SMa0421 - hypothetical protein, at 228,306 to 228,824
SMa0421
SMa5021: SMa5021 - hypothetical protein, at 228,821 to 229,255
SMa5021
SMa0424: SMa0424 - ATP-dependent DNA ligase, at 229,290 to 230,330
SMa0424
SMa0426: SMa0426 - hypothetical protein, at 230,327 to 231,133
SMa0426
Position (kb) 229
230
231 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 228.612 kb on + strand, within SMa0421 at 228.699 kb on + strand, within SMa0421 at 228.699 kb on + strand, within SMa0421 at 228.700 kb on - strand, within SMa0421 at 228.700 kb on - strand, within SMa0421 at 228.789 kb on + strand at 228.790 kb on - strand at 228.848 kb on + strand at 228.849 kb on - strand at 228.849 kb on - strand at 228.973 kb on - strand, within SMa5021 at 228.991 kb on - strand, within SMa5021 at 229.042 kb on + strand, within SMa5021 at 229.042 kb on + strand, within SMa5021 at 229.208 kb on + strand, within SMa5021 at 229.210 kb on + strand, within SMa5021 at 229.223 kb on - strand at 229.357 kb on + strand at 229.357 kb on + strand at 229.469 kb on + strand, within SMa0424 at 229.469 kb on + strand, within SMa0424 at 229.470 kb on - strand, within SMa0424 at 229.520 kb on + strand, within SMa0424 at 229.524 kb on - strand, within SMa0424 at 229.662 kb on + strand, within SMa0424 at 229.882 kb on + strand, within SMa0424 at 230.598 kb on + strand, within SMa0426 at 230.599 kb on - strand, within SMa0426 at 230.599 kb on - strand, within SMa0426 at 230.796 kb on + strand, within SMa0426 at 230.796 kb on + strand, within SMa0426 at 230.796 kb on + strand, within SMa0426 at 230.797 kb on - strand, within SMa0426 at 230.797 kb on - strand, within SMa0426 at 230.803 kb on + strand, within SMa0426 at 230.804 kb on - strand, within SMa0426 at 230.804 kb on - strand, within SMa0426 at 230.804 kb on - strand, within SMa0426 at 230.822 kb on + strand, within SMa0426 at 230.822 kb on + strand, within SMa0426 at 230.823 kb on - strand, within SMa0426 at 230.934 kb on + strand, within SMa0426 at 230.989 kb on - strand, within SMa0426 at 230.989 kb on - strand, within SMa0426 at 230.989 kb on - strand, within SMa0426 at 231.029 kb on - strand, within SMa0426 at 231.029 kb on - strand, within SMa0426 at 231.105 kb on + strand at 231.106 kb on - strand at 231.263 kb on + strand at 231.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 228,612 + SMa0421 0.59 -0.2 228,699 + SMa0421 0.76 -2.1 228,699 + SMa0421 0.76 -0.3 228,700 - SMa0421 0.76 +0.6 228,700 - SMa0421 0.76 -0.3 228,789 + -2.2 228,790 - -1.9 228,848 + +0.3 228,849 - -0.4 228,849 - -1.7 228,973 - SMa5021 0.35 -0.4 228,991 - SMa5021 0.39 +1.0 229,042 + SMa5021 0.51 +1.0 229,042 + SMa5021 0.51 +2.3 229,208 + SMa5021 0.89 -1.0 229,210 + SMa5021 0.89 -1.9 229,223 - -1.1 229,357 + -0.1 229,357 + +0.2 229,469 + SMa0424 0.17 +0.3 229,469 + SMa0424 0.17 -0.8 229,470 - SMa0424 0.17 -1.5 229,520 + SMa0424 0.22 -0.4 229,524 - SMa0424 0.22 -0.2 229,662 + SMa0424 0.36 -1.6 229,882 + SMa0424 0.57 +0.6 230,598 + SMa0426 0.34 -0.4 230,599 - SMa0426 0.34 -2.2 230,599 - SMa0426 0.34 -2.4 230,796 + SMa0426 0.58 +0.6 230,796 + SMa0426 0.58 +0.4 230,796 + SMa0426 0.58 -1.8 230,797 - SMa0426 0.58 +0.1 230,797 - SMa0426 0.58 -2.0 230,803 + SMa0426 0.59 +1.0 230,804 - SMa0426 0.59 -0.1 230,804 - SMa0426 0.59 +1.7 230,804 - SMa0426 0.59 -1.4 230,822 + SMa0426 0.61 -0.3 230,822 + SMa0426 0.61 +1.9 230,823 - SMa0426 0.61 -2.9 230,934 + SMa0426 0.75 +1.3 230,989 - SMa0426 0.82 -4.1 230,989 - SMa0426 0.82 +0.7 230,989 - SMa0426 0.82 +0.9 231,029 - SMa0426 0.87 +0.7 231,029 - SMa0426 0.87 -0.5 231,105 + +0.6 231,106 - +1.5 231,263 + -2.5 231,263 + -1.9
Or see this region's nucleotide sequence