Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20604 and SM_b20605 are separated by 142 nucleotides SM_b20605 and SM_b20606 are separated by 212 nucleotides SM_b20606 and SM_b20607 overlap by 4 nucleotides
SM_b20604: SM_b20604 - branched-chain amino acid ABC transporter permease, at 1,617,319 to 1,618,932
_b20604
SM_b20605: SM_b20605 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,619,075 to 1,620,367
_b20605
SM_b20606: SM_b20606 - hypothetical protein, at 1,620,580 to 1,621,029
_b20606
SM_b20607: SM_b20607 - hypothetical protein, at 1,621,026 to 1,621,490
_b20607
Position (kb)
1619
1620
1621 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1618.077 kb on + strand, within SM_b20604 at 1618.077 kb on + strand, within SM_b20604 at 1618.079 kb on + strand, within SM_b20604 at 1618.079 kb on + strand, within SM_b20604 at 1618.080 kb on - strand, within SM_b20604 at 1618.194 kb on - strand, within SM_b20604 at 1618.194 kb on - strand, within SM_b20604 at 1618.197 kb on + strand, within SM_b20604 at 1618.198 kb on + strand, within SM_b20604 at 1618.198 kb on + strand, within SM_b20604 at 1618.199 kb on - strand, within SM_b20604 at 1618.199 kb on - strand, within SM_b20604 at 1618.199 kb on - strand, within SM_b20604 at 1618.199 kb on - strand, within SM_b20604 at 1618.199 kb on - strand, within SM_b20604 at 1618.722 kb on - strand, within SM_b20604 at 1619.175 kb on + strand at 1619.175 kb on + strand at 1619.175 kb on + strand at 1619.176 kb on - strand at 1619.301 kb on + strand, within SM_b20605 at 1619.412 kb on + strand, within SM_b20605 at 1619.412 kb on + strand, within SM_b20605 at 1619.412 kb on + strand, within SM_b20605 at 1619.413 kb on - strand, within SM_b20605 at 1619.413 kb on - strand, within SM_b20605 at 1619.458 kb on + strand, within SM_b20605 at 1619.458 kb on + strand, within SM_b20605 at 1619.459 kb on - strand, within SM_b20605 at 1619.460 kb on + strand, within SM_b20605 at 1619.517 kb on + strand, within SM_b20605 at 1619.799 kb on + strand, within SM_b20605 at 1619.808 kb on + strand, within SM_b20605 at 1619.809 kb on - strand, within SM_b20605 at 1619.809 kb on - strand, within SM_b20605 at 1619.842 kb on - strand, within SM_b20605 at 1619.847 kb on + strand, within SM_b20605 at 1619.848 kb on - strand, within SM_b20605 at 1619.943 kb on + strand, within SM_b20605 at 1619.943 kb on + strand, within SM_b20605 at 1619.944 kb on - strand, within SM_b20605 at 1619.976 kb on + strand, within SM_b20605 at 1619.988 kb on + strand, within SM_b20605 at 1619.989 kb on - strand, within SM_b20605 at 1620.153 kb on - strand, within SM_b20605 at 1620.316 kb on + strand at 1620.362 kb on - strand at 1620.465 kb on + strand at 1620.466 kb on - strand at 1620.466 kb on - strand at 1620.476 kb on - strand at 1620.480 kb on - strand at 1620.588 kb on - strand at 1620.639 kb on - strand, within SM_b20606 at 1621.016 kb on + strand at 1621.016 kb on + strand at 1621.057 kb on + strand at 1621.058 kb on - strand at 1621.059 kb on - strand at 1621.262 kb on - strand, within SM_b20607 at 1621.262 kb on - strand, within SM_b20607 at 1621.279 kb on + strand, within SM_b20607 at 1621.280 kb on - strand, within SM_b20607 at 1621.280 kb on - strand, within SM_b20607
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,618,077 + SM_b20604 0.47 -1.4 1,618,077 + SM_b20604 0.47 -0.8 1,618,079 + SM_b20604 0.47 -1.4 1,618,079 + SM_b20604 0.47 +1.0 1,618,080 - SM_b20604 0.47 +0.4 1,618,194 - SM_b20604 0.54 -0.2 1,618,194 - SM_b20604 0.54 +0.6 1,618,197 + SM_b20604 0.54 -0.6 1,618,198 + SM_b20604 0.54 +1.2 1,618,198 + SM_b20604 0.54 +0.3 1,618,199 - SM_b20604 0.55 -1.0 1,618,199 - SM_b20604 0.55 -0.4 1,618,199 - SM_b20604 0.55 -1.2 1,618,199 - SM_b20604 0.55 +0.4 1,618,199 - SM_b20604 0.55 -1.0 1,618,722 - SM_b20604 0.87 -1.1 1,619,175 + -0.3 1,619,175 + -0.8 1,619,175 + -0.4 1,619,176 - -1.1 1,619,301 + SM_b20605 0.17 -0.3 1,619,412 + SM_b20605 0.26 -0.2 1,619,412 + SM_b20605 0.26 +2.6 1,619,412 + SM_b20605 0.26 -1.5 1,619,413 - SM_b20605 0.26 +0.0 1,619,413 - SM_b20605 0.26 +1.6 1,619,458 + SM_b20605 0.30 +2.1 1,619,458 + SM_b20605 0.30 +0.1 1,619,459 - SM_b20605 0.30 +0.1 1,619,460 + SM_b20605 0.30 +4.8 1,619,517 + SM_b20605 0.34 -0.1 1,619,799 + SM_b20605 0.56 -0.8 1,619,808 + SM_b20605 0.57 +1.0 1,619,809 - SM_b20605 0.57 -0.4 1,619,809 - SM_b20605 0.57 +0.1 1,619,842 - SM_b20605 0.59 +0.8 1,619,847 + SM_b20605 0.60 +0.3 1,619,848 - SM_b20605 0.60 -0.2 1,619,943 + SM_b20605 0.67 -1.3 1,619,943 + SM_b20605 0.67 -0.2 1,619,944 - SM_b20605 0.67 +0.2 1,619,976 + SM_b20605 0.70 +0.7 1,619,988 + SM_b20605 0.71 +0.4 1,619,989 - SM_b20605 0.71 -2.4 1,620,153 - SM_b20605 0.83 +0.3 1,620,316 + -1.2 1,620,362 - +0.5 1,620,465 + -3.2 1,620,466 - +0.2 1,620,466 - -0.3 1,620,476 - -3.3 1,620,480 - -2.3 1,620,588 - +0.2 1,620,639 - SM_b20606 0.13 -0.7 1,621,016 + +0.1 1,621,016 + +1.4 1,621,057 + +0.3 1,621,058 - +0.3 1,621,059 - -0.9 1,621,262 - SM_b20607 0.51 +0.9 1,621,262 - SM_b20607 0.51 +0.5 1,621,279 + SM_b20607 0.54 +0.1 1,621,280 - SM_b20607 0.55 -0.6 1,621,280 - SM_b20607 0.55 -0.1
Or see this region's nucleotide sequence