Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20194 and SM_b20195 are separated by 3 nucleotides SM_b20195 and SM_b20196 overlap by 4 nucleotides SM_b20196 and SM_b20197 are separated by 11 nucleotides SM_b20197 and SM_b20198 are separated by 41 nucleotides
SM_b20194: SM_b20194 - hypothetical protein, at 203,136 to 203,702
_b20194
SM_b20195: SM_b20195 - pentose-5-phosphate-3-epimerase, at 203,706 to 204,392
_b20195
SM_b20196: SM_b20196 - CbbX protein, at 204,389 to 205,324
_b20196
SM_b20197: SM_b20197 - ribulose-1,5-bisphosphate carboxylase small subunit (EC 4.1.1.39) <small>(from data)</small>, at 205,336 to 205,725
_b20197
SM_b20198: SM_b20198 - ribulose bisophosphate carboxylase, at 205,767 to 207,227
_b20198
Position (kb) 204
205
206 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 203.395 kb on + strand, within SM_b20194 at 203.395 kb on + strand, within SM_b20194 at 203.458 kb on + strand, within SM_b20194 at 203.557 kb on + strand, within SM_b20194 at 203.576 kb on + strand, within SM_b20194 at 203.576 kb on + strand, within SM_b20194 at 203.617 kb on - strand, within SM_b20194 at 203.693 kb on + strand at 203.767 kb on + strand at 203.768 kb on - strand at 203.877 kb on + strand, within SM_b20195 at 204.011 kb on - strand, within SM_b20195 at 204.011 kb on - strand, within SM_b20195 at 204.011 kb on - strand, within SM_b20195 at 204.163 kb on + strand, within SM_b20195 at 204.291 kb on + strand, within SM_b20195 at 204.291 kb on + strand, within SM_b20195 at 204.292 kb on - strand, within SM_b20195 at 204.603 kb on + strand, within SM_b20196 at 204.603 kb on + strand, within SM_b20196 at 204.603 kb on + strand, within SM_b20196 at 204.692 kb on - strand, within SM_b20196 at 204.711 kb on - strand, within SM_b20196 at 204.777 kb on + strand, within SM_b20196 at 204.885 kb on + strand, within SM_b20196 at 204.886 kb on - strand, within SM_b20196 at 204.886 kb on - strand, within SM_b20196 at 204.888 kb on + strand, within SM_b20196 at 204.889 kb on - strand, within SM_b20196 at 204.889 kb on - strand, within SM_b20196 at 204.964 kb on + strand, within SM_b20196 at 204.965 kb on - strand, within SM_b20196 at 204.966 kb on + strand, within SM_b20196 at 205.020 kb on + strand, within SM_b20196 at 205.020 kb on + strand, within SM_b20196 at 205.100 kb on - strand, within SM_b20196 at 205.100 kb on - strand, within SM_b20196 at 205.356 kb on + strand at 205.356 kb on + strand at 205.356 kb on + strand at 205.356 kb on + strand at 205.357 kb on - strand at 205.357 kb on - strand at 205.357 kb on - strand at 205.357 kb on - strand at 205.357 kb on - strand at 205.357 kb on - strand at 205.358 kb on + strand at 205.358 kb on + strand at 205.359 kb on - strand at 205.489 kb on - strand, within SM_b20197 at 205.490 kb on + strand, within SM_b20197 at 205.490 kb on + strand, within SM_b20197 at 205.490 kb on + strand, within SM_b20197 at 205.490 kb on + strand, within SM_b20197 at 205.490 kb on + strand, within SM_b20197 at 205.615 kb on - strand, within SM_b20197 at 205.652 kb on + strand, within SM_b20197 at 205.720 kb on - strand at 206.026 kb on - strand, within SM_b20198 at 206.026 kb on - strand, within SM_b20198 at 206.185 kb on + strand, within SM_b20198 at 206.186 kb on - strand, within SM_b20198 at 206.189 kb on - strand, within SM_b20198 at 206.318 kb on + strand, within SM_b20198 at 206.320 kb on + strand, within SM_b20198 at 206.321 kb on - strand, within SM_b20198
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 203,395 + SM_b20194 0.46 -1.9 203,395 + SM_b20194 0.46 +1.4 203,458 + SM_b20194 0.57 -0.9 203,557 + SM_b20194 0.74 -1.1 203,576 + SM_b20194 0.78 +1.0 203,576 + SM_b20194 0.78 +0.1 203,617 - SM_b20194 0.85 -0.9 203,693 + +0.1 203,767 + -0.2 203,768 - -0.5 203,877 + SM_b20195 0.25 +0.6 204,011 - SM_b20195 0.44 -0.1 204,011 - SM_b20195 0.44 +0.3 204,011 - SM_b20195 0.44 -1.8 204,163 + SM_b20195 0.67 -1.1 204,291 + SM_b20195 0.85 -0.3 204,291 + SM_b20195 0.85 -0.2 204,292 - SM_b20195 0.85 +0.8 204,603 + SM_b20196 0.23 -1.4 204,603 + SM_b20196 0.23 +0.7 204,603 + SM_b20196 0.23 -1.4 204,692 - SM_b20196 0.32 -1.2 204,711 - SM_b20196 0.34 +1.1 204,777 + SM_b20196 0.41 +0.0 204,885 + SM_b20196 0.53 -0.1 204,886 - SM_b20196 0.53 -0.3 204,886 - SM_b20196 0.53 -0.2 204,888 + SM_b20196 0.53 -0.6 204,889 - SM_b20196 0.53 -1.1 204,889 - SM_b20196 0.53 -1.5 204,964 + SM_b20196 0.61 -0.5 204,965 - SM_b20196 0.62 -0.2 204,966 + SM_b20196 0.62 -0.6 205,020 + SM_b20196 0.67 -0.3 205,020 + SM_b20196 0.67 +0.4 205,100 - SM_b20196 0.76 -0.2 205,100 - SM_b20196 0.76 -0.8 205,356 + +0.9 205,356 + -1.1 205,356 + +2.4 205,356 + -1.0 205,357 - -0.4 205,357 - -2.6 205,357 - +0.4 205,357 - +1.0 205,357 - +0.1 205,357 - -1.7 205,358 + -0.3 205,358 + -3.1 205,359 - -0.9 205,489 - SM_b20197 0.39 -0.1 205,490 + SM_b20197 0.39 +0.3 205,490 + SM_b20197 0.39 -1.3 205,490 + SM_b20197 0.39 +1.2 205,490 + SM_b20197 0.39 +0.1 205,490 + SM_b20197 0.39 -0.1 205,615 - SM_b20197 0.72 -1.0 205,652 + SM_b20197 0.81 -1.8 205,720 - -0.4 206,026 - SM_b20198 0.18 -0.8 206,026 - SM_b20198 0.18 +0.8 206,185 + SM_b20198 0.29 -0.5 206,186 - SM_b20198 0.29 -1.0 206,189 - SM_b20198 0.29 -0.2 206,318 + SM_b20198 0.38 +0.4 206,320 + SM_b20198 0.38 +3.7 206,321 - SM_b20198 0.38 -1.3
Or see this region's nucleotide sequence