Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20182 and SM_b20183 overlap by 4 nucleotides SM_b20183 and SM_b20184 overlap by 4 nucleotides SM_b20184 and SM_b20185 overlap by 4 nucleotides SM_b20185 and SM_b20186 are separated by 120 nucleotides
SM_b20182: SM_b20182 - ABC transporter, at 199,222 to 200,001
_b20182
SM_b20183: SM_b20183 - ABC transporter ATP-binding protein, at 199,998 to 200,621
_b20183
SM_b20184: SM_b20184 - ABC transporter ATP-binding protein, at 200,618 to 201,346
_b20184
SM_b20185: SM_b20185 - hypothetical protein, at 201,343 to 202,134
_b20185
SM_b20186: SM_b20186 - glutathione-dependent formaldehyde-activating protein, at 202,255 to 202,824
_b20186
Position (kb) 200
201
202 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 199.806 kb on - strand, within SM_b20182 at 199.873 kb on + strand, within SM_b20182 at 199.910 kb on - strand, within SM_b20182 at 199.910 kb on - strand, within SM_b20182 at 200.421 kb on + strand, within SM_b20183 at 200.582 kb on + strand at 200.584 kb on + strand at 200.585 kb on - strand at 200.585 kb on - strand at 200.585 kb on - strand at 201.081 kb on + strand, within SM_b20184 at 201.226 kb on - strand, within SM_b20184 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.518 kb on - strand, within SM_b20185 at 201.518 kb on - strand, within SM_b20185 at 201.519 kb on + strand, within SM_b20185 at 201.519 kb on + strand, within SM_b20185 at 201.520 kb on - strand, within SM_b20185 at 201.520 kb on - strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.535 kb on + strand, within SM_b20185 at 201.535 kb on + strand, within SM_b20185 at 201.536 kb on - strand at 201.537 kb on + strand, within SM_b20185 at 201.695 kb on + strand, within SM_b20185 at 201.965 kb on + strand, within SM_b20185 at 201.988 kb on + strand, within SM_b20185 at 201.989 kb on - strand, within SM_b20185 at 202.096 kb on + strand at 202.097 kb on - strand at 202.097 kb on - strand at 202.098 kb on + strand at 202.099 kb on - strand at 202.099 kb on - strand at 202.146 kb on - strand at 202.146 kb on - strand at 202.185 kb on + strand at 202.265 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 199,806 - SM_b20182 0.75 +0.2 199,873 + SM_b20182 0.83 +0.9 199,910 - SM_b20182 0.88 +0.5 199,910 - SM_b20182 0.88 -0.7 200,421 + SM_b20183 0.68 -0.5 200,582 + -2.0 200,584 + +0.6 200,585 - +1.0 200,585 - -2.1 200,585 - -0.4 201,081 + SM_b20184 0.64 -1.7 201,226 - SM_b20184 0.83 -0.4 201,517 + SM_b20185 0.22 -0.0 201,517 + SM_b20185 0.22 -1.9 201,517 + SM_b20185 0.22 -2.3 201,517 + SM_b20185 0.22 +0.3 201,517 + SM_b20185 0.22 -0.9 201,518 - SM_b20185 0.22 +0.3 201,518 - SM_b20185 0.22 +0.1 201,519 + SM_b20185 0.22 +0.6 201,519 + SM_b20185 0.22 +0.2 201,520 - SM_b20185 0.22 +0.3 201,520 - SM_b20185 0.22 -0.8 201,522 + SM_b20185 0.23 +1.0 201,522 + SM_b20185 0.23 -0.3 201,522 + SM_b20185 0.23 -2.6 201,522 + SM_b20185 0.23 -1.3 201,523 - SM_b20185 0.23 -0.9 201,523 - SM_b20185 0.23 -0.2 201,523 - SM_b20185 0.23 +0.6 201,523 - SM_b20185 0.23 -0.8 201,523 - SM_b20185 0.23 +0.4 201,526 + SM_b20185 0.23 -3.6 201,526 + SM_b20185 0.23 -0.2 201,526 + SM_b20185 0.23 -0.1 201,526 + SM_b20185 0.23 -0.1 201,535 + SM_b20185 0.24 -0.4 201,535 + SM_b20185 0.24 -2.1 201,536 - -0.8 201,537 + SM_b20185 0.24 -0.1 201,695 + SM_b20185 0.44 +2.0 201,965 + SM_b20185 0.79 -1.0 201,988 + SM_b20185 0.81 +0.3 201,989 - SM_b20185 0.82 +1.5 202,096 + -0.3 202,097 - +0.5 202,097 - -0.0 202,098 + +0.3 202,099 - -0.3 202,099 - -0.1 202,146 - -0.6 202,146 - +0.5 202,185 + -0.3 202,265 + -2.1
Or see this region's nucleotide sequence