Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20103 and SM_b20104 are separated by 75 nucleotides SM_b20104 and SM_b20105 are separated by 101 nucleotides SM_b20105 and SM_b20106 are separated by 179 nucleotides SM_b20106 and SM_b20107 are separated by 67 nucleotides
SM_b20103: SM_b20103 - FAD-dependent oxidoreductase, at 113,274 to 115,076
_b20103
SM_b20104: SM_b20104 - hypothetical protein, at 115,152 to 115,910
_b20104
SM_b20105: SM_b20105 - transcriptional regulator, at 116,012 to 116,644
_b20105
SM_b20106: SM_b20106 - transcriptional regulator, at 116,824 to 117,555
_b20106
SM_b20107: SM_b20107 - hypothetical protein, at 117,623 to 118,579
_b20107
Position (kb)
116
117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 115.031 kb on - strand at 115.031 kb on - strand at 115.045 kb on - strand at 115.254 kb on - strand, within SM_b20104 at 115.367 kb on + strand, within SM_b20104 at 115.367 kb on + strand, within SM_b20104 at 115.367 kb on + strand, within SM_b20104 at 115.368 kb on - strand, within SM_b20104 at 115.369 kb on + strand, within SM_b20104 at 115.369 kb on + strand, within SM_b20104 at 115.369 kb on + strand, within SM_b20104 at 115.369 kb on + strand, within SM_b20104 at 115.370 kb on - strand, within SM_b20104 at 115.370 kb on - strand, within SM_b20104 at 115.370 kb on - strand, within SM_b20104 at 115.423 kb on - strand, within SM_b20104 at 115.440 kb on - strand, within SM_b20104 at 115.541 kb on + strand, within SM_b20104 at 115.541 kb on + strand at 115.541 kb on + strand, within SM_b20104 at 115.541 kb on + strand, within SM_b20104 at 115.542 kb on - strand, within SM_b20104 at 115.580 kb on - strand, within SM_b20104 at 115.588 kb on - strand, within SM_b20104 at 115.900 kb on + strand at 115.901 kb on - strand at 115.983 kb on + strand at 115.983 kb on + strand at 115.984 kb on - strand at 115.984 kb on - strand at 116.013 kb on + strand at 116.024 kb on + strand at 116.024 kb on + strand at 116.025 kb on - strand at 116.025 kb on - strand at 116.025 kb on - strand at 116.025 kb on - strand at 116.456 kb on + strand, within SM_b20105 at 116.495 kb on + strand, within SM_b20105 at 116.915 kb on + strand, within SM_b20106 at 116.916 kb on - strand, within SM_b20106 at 117.064 kb on + strand, within SM_b20106 at 117.064 kb on + strand, within SM_b20106 at 117.064 kb on + strand, within SM_b20106 at 117.064 kb on + strand, within SM_b20106 at 117.065 kb on - strand, within SM_b20106 at 117.065 kb on - strand, within SM_b20106 at 117.117 kb on + strand, within SM_b20106 at 117.118 kb on - strand, within SM_b20106 at 117.154 kb on + strand, within SM_b20106 at 117.155 kb on - strand, within SM_b20106 at 117.155 kb on - strand, within SM_b20106 at 117.480 kb on - strand, within SM_b20106 at 117.540 kb on + strand at 117.540 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 115,031 - -0.7 115,031 - +0.3 115,045 - +0.6 115,254 - SM_b20104 0.13 +0.5 115,367 + SM_b20104 0.28 -2.7 115,367 + SM_b20104 0.28 -0.5 115,367 + SM_b20104 0.28 -1.1 115,368 - SM_b20104 0.28 -1.7 115,369 + SM_b20104 0.29 -0.5 115,369 + SM_b20104 0.29 -1.4 115,369 + SM_b20104 0.29 -0.4 115,369 + SM_b20104 0.29 +0.1 115,370 - SM_b20104 0.29 +0.4 115,370 - SM_b20104 0.29 +0.6 115,370 - SM_b20104 0.29 -2.2 115,423 - SM_b20104 0.36 -1.2 115,440 - SM_b20104 0.38 +0.6 115,541 + SM_b20104 0.51 +0.3 115,541 + +2.4 115,541 + SM_b20104 0.51 -1.0 115,541 + SM_b20104 0.51 +0.6 115,542 - SM_b20104 0.51 +0.7 115,580 - SM_b20104 0.56 +1.4 115,588 - SM_b20104 0.57 -1.2 115,900 + +0.3 115,901 - -0.3 115,983 + +1.2 115,983 + +0.1 115,984 - -1.5 115,984 - -0.5 116,013 + -1.5 116,024 + +0.7 116,024 + -2.8 116,025 - -0.1 116,025 - -1.0 116,025 - -1.7 116,025 - -0.0 116,456 + SM_b20105 0.70 -2.5 116,495 + SM_b20105 0.76 -2.2 116,915 + SM_b20106 0.12 -1.4 116,916 - SM_b20106 0.13 +0.7 117,064 + SM_b20106 0.33 +0.3 117,064 + SM_b20106 0.33 -0.9 117,064 + SM_b20106 0.33 +0.5 117,064 + SM_b20106 0.33 -2.7 117,065 - SM_b20106 0.33 -0.2 117,065 - SM_b20106 0.33 -0.1 117,117 + SM_b20106 0.40 -1.1 117,118 - SM_b20106 0.40 +0.6 117,154 + SM_b20106 0.45 -0.3 117,155 - SM_b20106 0.45 -0.4 117,155 - SM_b20106 0.45 +0.1 117,480 - SM_b20106 0.90 -0.9 117,540 + +0.5 117,540 + +0.6
Or see this region's nucleotide sequence