Experiment: Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4012 and Pf6N2E2_4013 are separated by 138 nucleotides Pf6N2E2_4013 and Pf6N2E2_4014 are separated by 173 nucleotides
Pf6N2E2_4012 - response regulator, at 4,683,332 to 4,684,549
_4012
Pf6N2E2_4013 - 5-aminovalerate transaminase (EC 2.6.1.48) (from data) , at 4,684,688 to 4,685,965
_4013
Pf6N2E2_4014 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 4,686,139 to 4,687,581
_4014
Position (kb)
4684
4685
4686 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4683.696 kb on + strand, within Pf6N2E2_4012 at 4683.697 kb on - strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.384 kb on - strand, within Pf6N2E2_4012 at 4684.384 kb on - strand, within Pf6N2E2_4012 at 4684.524 kb on - strand at 4684.569 kb on + strand at 4684.569 kb on + strand at 4684.569 kb on + strand at 4684.570 kb on - strand at 4684.570 kb on - strand at 4684.570 kb on - strand at 4684.570 kb on - strand at 4684.580 kb on + strand at 4684.581 kb on - strand at 4684.634 kb on + strand at 4684.635 kb on - strand at 4684.785 kb on + strand at 4684.785 kb on + strand at 4684.786 kb on - strand at 4684.786 kb on - strand at 4684.786 kb on - strand at 4684.893 kb on - strand, within Pf6N2E2_4013 at 4684.938 kb on + strand, within Pf6N2E2_4013 at 4684.939 kb on - strand, within Pf6N2E2_4013 at 4685.070 kb on + strand, within Pf6N2E2_4013 at 4685.070 kb on + strand, within Pf6N2E2_4013 at 4685.070 kb on + strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.109 kb on + strand, within Pf6N2E2_4013 at 4685.109 kb on + strand, within Pf6N2E2_4013 at 4685.109 kb on + strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.315 kb on + strand, within Pf6N2E2_4013 at 4685.315 kb on + strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.339 kb on + strand, within Pf6N2E2_4013 at 4685.451 kb on + strand, within Pf6N2E2_4013 at 4685.488 kb on + strand, within Pf6N2E2_4013 at 4685.500 kb on - strand, within Pf6N2E2_4013 at 4685.500 kb on - strand, within Pf6N2E2_4013 at 4685.706 kb on + strand, within Pf6N2E2_4013 at 4685.706 kb on + strand, within Pf6N2E2_4013 at 4685.706 kb on + strand, within Pf6N2E2_4013 at 4685.715 kb on + strand, within Pf6N2E2_4013 at 4685.929 kb on + strand at 4685.930 kb on - strand at 4685.966 kb on + strand at 4685.967 kb on - strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.158 kb on + strand at 4686.158 kb on + strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.174 kb on - strand at 4686.188 kb on + strand at 4686.188 kb on + strand at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.563 kb on - strand, within Pf6N2E2_4014 at 4686.677 kb on + strand, within Pf6N2E2_4014 at 4686.677 kb on + strand, within Pf6N2E2_4014 at 4686.677 kb on + strand, within Pf6N2E2_4014 at 4686.695 kb on + strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.768 kb on + strand, within Pf6N2E2_4014 at 4686.796 kb on - strand, within Pf6N2E2_4014
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03 mg/ml remove 4,683,696 + Pf6N2E2_4012 0.30 -0.3 4,683,697 - Pf6N2E2_4012 0.30 +0.2 4,684,383 + Pf6N2E2_4012 0.86 +0.9 4,684,383 + Pf6N2E2_4012 0.86 +0.5 4,684,383 + Pf6N2E2_4012 0.86 +0.6 4,684,383 + Pf6N2E2_4012 0.86 +0.2 4,684,384 - Pf6N2E2_4012 0.86 +0.1 4,684,384 - Pf6N2E2_4012 0.86 -0.0 4,684,524 - +0.0 4,684,569 + +0.6 4,684,569 + -1.0 4,684,569 + +0.0 4,684,570 - -0.0 4,684,570 - +0.4 4,684,570 - -1.0 4,684,570 - +0.9 4,684,580 + +0.7 4,684,581 - +0.0 4,684,634 + +0.1 4,684,635 - +1.0 4,684,785 + +1.1 4,684,785 + +1.0 4,684,786 - +2.0 4,684,786 - +0.4 4,684,786 - +1.8 4,684,893 - Pf6N2E2_4013 0.16 +1.7 4,684,938 + Pf6N2E2_4013 0.20 +2.0 4,684,939 - Pf6N2E2_4013 0.20 +1.5 4,685,070 + Pf6N2E2_4013 0.30 +2.5 4,685,070 + Pf6N2E2_4013 0.30 +2.0 4,685,070 + Pf6N2E2_4013 0.30 +1.7 4,685,071 - Pf6N2E2_4013 0.30 +1.6 4,685,071 - Pf6N2E2_4013 0.30 -0.3 4,685,071 - Pf6N2E2_4013 0.30 +1.0 4,685,071 - Pf6N2E2_4013 0.30 +2.3 4,685,071 - Pf6N2E2_4013 0.30 +2.0 4,685,109 + Pf6N2E2_4013 0.33 +1.9 4,685,109 + Pf6N2E2_4013 0.33 +1.9 4,685,109 + Pf6N2E2_4013 0.33 +1.5 4,685,110 - Pf6N2E2_4013 0.33 +2.0 4,685,110 - Pf6N2E2_4013 0.33 +2.3 4,685,110 - Pf6N2E2_4013 0.33 +1.9 4,685,110 - Pf6N2E2_4013 0.33 +1.5 4,685,315 + Pf6N2E2_4013 0.49 +3.4 4,685,315 + Pf6N2E2_4013 0.49 +2.1 4,685,316 - Pf6N2E2_4013 0.49 +2.0 4,685,316 - Pf6N2E2_4013 0.49 +1.3 4,685,316 - Pf6N2E2_4013 0.49 +1.0 4,685,316 - Pf6N2E2_4013 0.49 +2.6 4,685,316 - Pf6N2E2_4013 0.49 +2.7 4,685,316 - Pf6N2E2_4013 0.49 +1.6 4,685,316 - Pf6N2E2_4013 0.49 +1.7 4,685,316 - Pf6N2E2_4013 0.49 +1.4 4,685,319 + Pf6N2E2_4013 0.49 +0.4 4,685,319 + Pf6N2E2_4013 0.49 +0.7 4,685,319 + Pf6N2E2_4013 0.49 +1.6 4,685,319 + Pf6N2E2_4013 0.49 +2.6 4,685,319 + Pf6N2E2_4013 0.49 +2.0 4,685,319 + Pf6N2E2_4013 0.49 +0.9 4,685,319 + Pf6N2E2_4013 0.49 +1.8 4,685,319 + Pf6N2E2_4013 0.49 +1.7 4,685,319 + Pf6N2E2_4013 0.49 +1.3 4,685,320 - Pf6N2E2_4013 0.49 +1.6 4,685,320 - Pf6N2E2_4013 0.49 +1.6 4,685,320 - Pf6N2E2_4013 0.49 +1.4 4,685,320 - Pf6N2E2_4013 0.49 +2.2 4,685,320 - Pf6N2E2_4013 0.49 +1.6 4,685,320 - Pf6N2E2_4013 0.49 +0.4 4,685,339 + Pf6N2E2_4013 0.51 +1.7 4,685,451 + Pf6N2E2_4013 0.60 +1.6 4,685,488 + Pf6N2E2_4013 0.63 +1.9 4,685,500 - Pf6N2E2_4013 0.64 +1.2 4,685,500 - Pf6N2E2_4013 0.64 +1.2 4,685,706 + Pf6N2E2_4013 0.80 +2.5 4,685,706 + Pf6N2E2_4013 0.80 +2.1 4,685,706 + Pf6N2E2_4013 0.80 +1.3 4,685,715 + Pf6N2E2_4013 0.80 +1.1 4,685,929 + +1.7 4,685,930 - +2.1 4,685,966 + +2.1 4,685,967 - +1.5 4,686,012 + +1.2 4,686,012 + +1.6 4,686,012 + +1.1 4,686,012 + +1.6 4,686,012 + +1.1 4,686,013 - +0.1 4,686,013 - +0.3 4,686,013 - -0.3 4,686,013 - -1.9 4,686,013 - -0.6 4,686,013 - -0.2 4,686,013 - -0.4 4,686,013 - -0.1 4,686,013 - +0.1 4,686,013 - -0.5 4,686,013 - -0.7 4,686,158 + +1.8 4,686,158 + +1.0 4,686,159 - +2.0 4,686,159 - +1.2 4,686,159 - +2.6 4,686,159 - +2.4 4,686,159 - +2.0 4,686,174 - +2.0 4,686,188 + +2.5 4,686,188 + +1.5 4,686,332 + Pf6N2E2_4014 0.13 +2.1 4,686,332 + Pf6N2E2_4014 0.13 +1.4 4,686,332 + Pf6N2E2_4014 0.13 +0.8 4,686,332 + Pf6N2E2_4014 0.13 +1.8 4,686,332 + Pf6N2E2_4014 0.13 +2.5 4,686,333 - Pf6N2E2_4014 0.13 +2.0 4,686,333 - Pf6N2E2_4014 0.13 +2.5 4,686,333 - Pf6N2E2_4014 0.13 +1.7 4,686,333 - Pf6N2E2_4014 0.13 +1.2 4,686,333 - Pf6N2E2_4014 0.13 +2.3 4,686,333 - Pf6N2E2_4014 0.13 +2.2 4,686,338 + Pf6N2E2_4014 0.14 +0.8 4,686,338 + Pf6N2E2_4014 0.14 +2.5 4,686,338 + Pf6N2E2_4014 0.14 +1.9 4,686,338 + Pf6N2E2_4014 0.14 +2.9 4,686,338 + Pf6N2E2_4014 0.14 +1.8 4,686,338 + Pf6N2E2_4014 0.14 +2.1 4,686,562 + Pf6N2E2_4014 0.29 +1.8 4,686,562 + Pf6N2E2_4014 0.29 +1.8 4,686,562 + Pf6N2E2_4014 0.29 +1.4 4,686,562 + Pf6N2E2_4014 0.29 +2.1 4,686,563 - Pf6N2E2_4014 0.29 +1.9 4,686,677 + Pf6N2E2_4014 0.37 +1.6 4,686,677 + Pf6N2E2_4014 0.37 +1.2 4,686,677 + Pf6N2E2_4014 0.37 +2.1 4,686,695 + Pf6N2E2_4014 0.39 +2.6 4,686,696 - Pf6N2E2_4014 0.39 +2.2 4,686,696 - Pf6N2E2_4014 0.39 +2.3 4,686,696 - Pf6N2E2_4014 0.39 +1.4 4,686,696 - Pf6N2E2_4014 0.39 +1.9 4,686,768 + Pf6N2E2_4014 0.44 +2.3 4,686,796 - Pf6N2E2_4014 0.46 +1.7
Or see this region's nucleotide sequence