Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5680 and Pf1N1B4_5681 are separated by 7 nucleotides Pf1N1B4_5681 and Pf1N1B4_5682 overlap by 1 nucleotides Pf1N1B4_5682 and Pf1N1B4_5683 are separated by 87 nucleotides
Pf1N1B4_5680 - DNA polymerase-like protein PA0670, at 119,869 to 121,284
_5680
Pf1N1B4_5681 - RecA/RadA recombinase, at 121,292 to 121,909
_5681
Pf1N1B4_5682 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 121,909 to 122,526
_5682
Pf1N1B4_5683 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 122,614 to 123,501
_5683
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-2
-1
0
1 at 121.071 kb on - strand, within Pf1N1B4_5680 at 121.986 kb on - strand, within Pf1N1B4_5682 at 122.002 kb on + strand at 122.134 kb on - strand, within Pf1N1B4_5682 at 122.174 kb on + strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682 at 122.507 kb on - strand at 122.507 kb on - strand at 122.507 kb on - strand at 122.673 kb on - strand at 123.362 kb on - strand, within Pf1N1B4_5683 at 123.444 kb on + strand at 123.444 kb on + strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.494 kb on + strand at 123.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 121,071 - Pf1N1B4_5680 0.85 -1.3 121,986 - Pf1N1B4_5682 0.12 -1.7 122,002 + +0.2 122,134 - Pf1N1B4_5682 0.36 -1.1 122,174 + Pf1N1B4_5682 0.43 -0.5 122,175 - Pf1N1B4_5682 0.43 +0.0 122,175 - Pf1N1B4_5682 0.43 -1.2 122,507 - -1.4 122,507 - +0.7 122,507 - -0.5 122,673 - +0.9 123,362 - Pf1N1B4_5683 0.84 -1.9 123,444 + -1.0 123,444 + -1.4 123,445 - -1.7 123,445 - +0.5 123,445 - +1.5 123,445 - +0.2 123,494 + -1.4 123,495 - -0.4
Or see this region's nucleotide sequence