Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5048

Experiment: Cobalt chloride 0.3125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5047 and Pf1N1B4_5048 are separated by 246 nucleotidesPf1N1B4_5048 and Pf1N1B4_5049 are separated by 127 nucleotidesPf1N1B4_5049 and Pf1N1B4_5050 are separated by 12 nucleotidesPf1N1B4_5050 and Pf1N1B4_5051 are separated by 44 nucleotides Pf1N1B4_5047 - Integral membrane protein TerC, at 5,591,536 to 5,592,507 _5047 Pf1N1B4_5048 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 5,592,754 to 5,593,680 _5048 Pf1N1B4_5049 - FIG00959942: hypothetical protein, at 5,593,808 to 5,594,326 _5049 Pf1N1B4_5050 - hypothetical protein, at 5,594,339 to 5,594,452 _5050 Pf1N1B4_5051 - Sensor protein DegS, at 5,594,497 to 5,595,681 _5051 Position (kb) 5592 5593 5594Strain fitness (log2 ratio) -2 -1 0 1 2at 5591.974 kb on + strandat 5591.975 kb on - strand, within Pf1N1B4_5047at 5592.048 kb on + strand, within Pf1N1B4_5047at 5592.059 kb on + strand, within Pf1N1B4_5047at 5592.071 kb on + strand, within Pf1N1B4_5047at 5592.295 kb on + strand, within Pf1N1B4_5047at 5592.356 kb on + strand, within Pf1N1B4_5047at 5592.612 kb on - strandat 5592.661 kb on - strandat 5592.661 kb on - strandat 5592.865 kb on + strand, within Pf1N1B4_5048at 5592.917 kb on - strandat 5592.917 kb on - strand, within Pf1N1B4_5048at 5592.917 kb on - strand, within Pf1N1B4_5048at 5593.517 kb on - strandat 5593.588 kb on + strandat 5593.905 kb on + strand, within Pf1N1B4_5049at 5594.055 kb on + strand, within Pf1N1B4_5049at 5594.056 kb on - strand, within Pf1N1B4_5049at 5594.056 kb on - strand, within Pf1N1B4_5049at 5594.248 kb on - strand, within Pf1N1B4_5049at 5594.248 kb on - strand, within Pf1N1B4_5049at 5594.248 kb on - strand, within Pf1N1B4_5049at 5594.248 kb on - strand, within Pf1N1B4_5049at 5594.248 kb on - strandat 5594.248 kb on - strand, within Pf1N1B4_5049at 5594.450 kb on + strandat 5594.451 kb on - strandat 5594.490 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM
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5,591,974 + +0.8
5,591,975 - Pf1N1B4_5047 0.45 +0.6
5,592,048 + Pf1N1B4_5047 0.53 -0.2
5,592,059 + Pf1N1B4_5047 0.54 -0.3
5,592,071 + Pf1N1B4_5047 0.55 +1.1
5,592,295 + Pf1N1B4_5047 0.78 -0.5
5,592,356 + Pf1N1B4_5047 0.84 +0.1
5,592,612 - -1.5
5,592,661 - +0.5
5,592,661 - -0.2
5,592,865 + Pf1N1B4_5048 0.12 +0.5
5,592,917 - -0.2
5,592,917 - Pf1N1B4_5048 0.18 -0.9
5,592,917 - Pf1N1B4_5048 0.18 -2.0
5,593,517 - -0.2
5,593,588 + +0.1
5,593,905 + Pf1N1B4_5049 0.19 +0.1
5,594,055 + Pf1N1B4_5049 0.48 -0.2
5,594,056 - Pf1N1B4_5049 0.48 +1.8
5,594,056 - Pf1N1B4_5049 0.48 -0.6
5,594,248 - Pf1N1B4_5049 0.85 -0.4
5,594,248 - Pf1N1B4_5049 0.85 -0.6
5,594,248 - Pf1N1B4_5049 0.85 -2.4
5,594,248 - Pf1N1B4_5049 0.85 +0.3
5,594,248 - -1.2
5,594,248 - Pf1N1B4_5049 0.85 -1.6
5,594,450 + -0.3
5,594,451 - -0.6
5,594,490 + +0.3

Or see this region's nucleotide sequence