Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5047 and Pf1N1B4_5048 are separated by 246 nucleotides Pf1N1B4_5048 and Pf1N1B4_5049 are separated by 127 nucleotides Pf1N1B4_5049 and Pf1N1B4_5050 are separated by 12 nucleotides Pf1N1B4_5050 and Pf1N1B4_5051 are separated by 44 nucleotides
Pf1N1B4_5047 - Integral membrane protein TerC, at 5,591,536 to 5,592,507
_5047
Pf1N1B4_5048 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 5,592,754 to 5,593,680
_5048
Pf1N1B4_5049 - FIG00959942: hypothetical protein, at 5,593,808 to 5,594,326
_5049
Pf1N1B4_5050 - hypothetical protein, at 5,594,339 to 5,594,452
_5050
Pf1N1B4_5051 - Sensor protein DegS, at 5,594,497 to 5,595,681
_5051
Position (kb)
5592
5593
5594 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5591.974 kb on + strand at 5591.975 kb on - strand, within Pf1N1B4_5047 at 5592.048 kb on + strand, within Pf1N1B4_5047 at 5592.059 kb on + strand, within Pf1N1B4_5047 at 5592.071 kb on + strand, within Pf1N1B4_5047 at 5592.295 kb on + strand, within Pf1N1B4_5047 at 5592.356 kb on + strand, within Pf1N1B4_5047 at 5592.612 kb on - strand at 5592.661 kb on - strand at 5592.661 kb on - strand at 5592.865 kb on + strand, within Pf1N1B4_5048 at 5592.917 kb on - strand at 5592.917 kb on - strand, within Pf1N1B4_5048 at 5592.917 kb on - strand, within Pf1N1B4_5048 at 5593.517 kb on - strand at 5593.588 kb on + strand at 5593.905 kb on + strand, within Pf1N1B4_5049 at 5594.055 kb on + strand, within Pf1N1B4_5049 at 5594.056 kb on - strand, within Pf1N1B4_5049 at 5594.056 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.450 kb on + strand at 5594.451 kb on - strand at 5594.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 5,591,974 + +0.8 5,591,975 - Pf1N1B4_5047 0.45 +0.6 5,592,048 + Pf1N1B4_5047 0.53 -0.2 5,592,059 + Pf1N1B4_5047 0.54 -0.3 5,592,071 + Pf1N1B4_5047 0.55 +1.1 5,592,295 + Pf1N1B4_5047 0.78 -0.5 5,592,356 + Pf1N1B4_5047 0.84 +0.1 5,592,612 - -1.5 5,592,661 - +0.5 5,592,661 - -0.2 5,592,865 + Pf1N1B4_5048 0.12 +0.5 5,592,917 - -0.2 5,592,917 - Pf1N1B4_5048 0.18 -0.9 5,592,917 - Pf1N1B4_5048 0.18 -2.0 5,593,517 - -0.2 5,593,588 + +0.1 5,593,905 + Pf1N1B4_5049 0.19 +0.1 5,594,055 + Pf1N1B4_5049 0.48 -0.2 5,594,056 - Pf1N1B4_5049 0.48 +1.8 5,594,056 - Pf1N1B4_5049 0.48 -0.6 5,594,248 - Pf1N1B4_5049 0.85 -0.4 5,594,248 - Pf1N1B4_5049 0.85 -0.6 5,594,248 - Pf1N1B4_5049 0.85 -2.4 5,594,248 - Pf1N1B4_5049 0.85 +0.3 5,594,248 - -1.2 5,594,248 - Pf1N1B4_5049 0.85 -1.6 5,594,450 + -0.3 5,594,451 - -0.6 5,594,490 + +0.3
Or see this region's nucleotide sequence