Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4809 and Pf1N1B4_4810 overlap by 4 nucleotides Pf1N1B4_4810 and Pf1N1B4_4811 overlap by 4 nucleotides Pf1N1B4_4811 and Pf1N1B4_4812 are separated by 575 nucleotides
Pf1N1B4_4809 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 5,312,015 to 5,313,394
_4809
Pf1N1B4_4810 - Polymyxin resistance protein PmrM, at 5,313,391 to 5,313,804
_4810
Pf1N1B4_4811 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 5,313,801 to 5,314,145
_4811
Pf1N1B4_4812 - Site-specific recombinase, phage integrase family, at 5,314,721 to 5,315,692
_4812
Position (kb)
5313
5314 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5312.466 kb on + strand, within Pf1N1B4_4809 at 5312.466 kb on + strand, within Pf1N1B4_4809 at 5313.367 kb on - strand at 5313.381 kb on - strand at 5313.530 kb on + strand at 5313.530 kb on + strand, within Pf1N1B4_4810 at 5313.543 kb on - strand, within Pf1N1B4_4810 at 5313.563 kb on + strand, within Pf1N1B4_4810 at 5313.663 kb on + strand, within Pf1N1B4_4810 at 5314.129 kb on - strand at 5314.356 kb on - strand at 5314.379 kb on + strand at 5314.379 kb on + strand at 5314.380 kb on - strand at 5314.380 kb on - strand at 5314.663 kb on - strand at 5314.691 kb on - strand at 5314.693 kb on - strand at 5314.700 kb on - strand at 5314.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 5,312,466 + Pf1N1B4_4809 0.33 +0.4 5,312,466 + Pf1N1B4_4809 0.33 +1.9 5,313,367 - -0.8 5,313,381 - +0.1 5,313,530 + -0.5 5,313,530 + Pf1N1B4_4810 0.34 +1.4 5,313,543 - Pf1N1B4_4810 0.37 -1.9 5,313,563 + Pf1N1B4_4810 0.42 -2.3 5,313,663 + Pf1N1B4_4810 0.66 -1.7 5,314,129 - -0.8 5,314,356 - -1.8 5,314,379 + +1.4 5,314,379 + -0.2 5,314,380 - -0.8 5,314,380 - -0.2 5,314,663 - +0.8 5,314,691 - -0.2 5,314,693 - -0.9 5,314,700 - +0.3 5,314,765 - -2.3
Or see this region's nucleotide sequence