Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4784 and Pf1N1B4_4785 are separated by 16 nucleotides Pf1N1B4_4785 and Pf1N1B4_4786 are separated by 12 nucleotides Pf1N1B4_4786 and Pf1N1B4_4787 are separated by 165 nucleotides
Pf1N1B4_4784 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 5,285,831 to 5,287,495
_4784
Pf1N1B4_4785 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 5,287,512 to 5,288,273
_4785
Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479
_4786
Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,289,645 to 5,290,772
_4787
Position (kb)
5288
5289
5290 Strain fitness (log2 ratio)
-2
-1
0
1 at 5287.432 kb on + strand at 5288.182 kb on - strand, within Pf1N1B4_4785 at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.206 kb on + strand at 5288.206 kb on + strand at 5288.271 kb on + strand at 5288.272 kb on - strand at 5288.272 kb on - strand at 5288.281 kb on + strand at 5288.282 kb on - strand at 5288.282 kb on - strand at 5288.282 kb on - strand at 5288.312 kb on + strand at 5288.313 kb on - strand at 5288.313 kb on - strand at 5288.313 kb on - strand at 5289.036 kb on - strand at 5289.474 kb on + strand at 5289.474 kb on + strand at 5289.617 kb on + strand at 5289.761 kb on + strand at 5289.762 kb on - strand, within Pf1N1B4_4787 at 5289.917 kb on - strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.215 kb on + strand, within Pf1N1B4_4787 at 5290.218 kb on - strand, within Pf1N1B4_4787 at 5290.218 kb on - strand at 5290.262 kb on + strand, within Pf1N1B4_4787
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 5,287,432 + +0.8 5,288,182 - Pf1N1B4_4785 0.88 +0.6 5,288,202 - -1.2 5,288,202 - +1.1 5,288,202 - -0.4 5,288,202 - -1.1 5,288,206 + +0.8 5,288,206 + +0.4 5,288,271 + +0.6 5,288,272 - -0.1 5,288,272 - +0.6 5,288,281 + -0.1 5,288,282 - -0.4 5,288,282 - -0.5 5,288,282 - +1.1 5,288,312 + +0.5 5,288,313 - +1.1 5,288,313 - +0.2 5,288,313 - +0.2 5,289,036 - +0.8 5,289,474 + +0.2 5,289,474 + -0.9 5,289,617 + +1.0 5,289,761 + -0.5 5,289,762 - Pf1N1B4_4787 0.10 -0.6 5,289,917 - Pf1N1B4_4787 0.24 -0.4 5,290,080 + Pf1N1B4_4787 0.39 +0.8 5,290,080 + Pf1N1B4_4787 0.39 +0.1 5,290,215 + Pf1N1B4_4787 0.51 -2.6 5,290,218 - Pf1N1B4_4787 0.51 +0.0 5,290,218 - -0.2 5,290,262 + Pf1N1B4_4787 0.55 -0.2
Or see this region's nucleotide sequence