Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4447 and Pf1N1B4_4448 are separated by 149 nucleotides Pf1N1B4_4448 and Pf1N1B4_4449 are separated by 167 nucleotides Pf1N1B4_4449 and Pf1N1B4_4450 are separated by 179 nucleotides
Pf1N1B4_4447 - FIG00904572: hypothetical protein, at 4,907,018 to 4,907,347
_4447
Pf1N1B4_4448 - COGs COG2343, at 4,907,497 to 4,907,859
_4448
Pf1N1B4_4449 - Permeases of the major facilitator superfamily, at 4,908,027 to 4,909,649
_4449
Pf1N1B4_4450 - Choline dehydrogenase (EC 1.1.99.1), at 4,909,829 to 4,911,478
_4450
Position (kb)
4908
4909
4910 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4907.215 kb on - strand, within Pf1N1B4_4447 at 4907.446 kb on - strand at 4907.447 kb on + strand at 4907.447 kb on + strand at 4907.448 kb on - strand at 4907.448 kb on - strand at 4907.448 kb on - strand at 4907.491 kb on + strand at 4907.491 kb on + strand at 4907.491 kb on + strand at 4907.491 kb on + strand at 4907.491 kb on + strand at 4907.492 kb on - strand at 4907.492 kb on - strand at 4907.496 kb on + strand at 4907.497 kb on - strand at 4907.635 kb on + strand, within Pf1N1B4_4448 at 4907.635 kb on + strand, within Pf1N1B4_4448 at 4907.636 kb on - strand at 4907.636 kb on - strand, within Pf1N1B4_4448 at 4907.701 kb on + strand at 4907.719 kb on + strand, within Pf1N1B4_4448 at 4907.719 kb on + strand, within Pf1N1B4_4448 at 4907.770 kb on + strand, within Pf1N1B4_4448 at 4907.863 kb on - strand at 4908.143 kb on - strand at 4908.407 kb on - strand at 4908.475 kb on + strand, within Pf1N1B4_4449 at 4908.838 kb on + strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4909.097 kb on - strand, within Pf1N1B4_4449 at 4909.207 kb on + strand, within Pf1N1B4_4449 at 4909.207 kb on + strand at 4909.208 kb on - strand, within Pf1N1B4_4449 at 4909.304 kb on - strand, within Pf1N1B4_4449 at 4909.369 kb on + strand, within Pf1N1B4_4449 at 4909.528 kb on + strand at 4909.729 kb on - strand at 4909.729 kb on - strand at 4909.740 kb on + strand at 4909.740 kb on + strand at 4909.740 kb on + strand at 4909.741 kb on - strand at 4909.741 kb on - strand at 4909.754 kb on + strand at 4909.755 kb on - strand at 4909.830 kb on + strand at 4909.830 kb on + strand at 4909.830 kb on + strand at 4909.936 kb on + strand at 4909.937 kb on - strand at 4910.140 kb on - strand, within Pf1N1B4_4450 at 4910.213 kb on + strand, within Pf1N1B4_4450 at 4910.241 kb on + strand, within Pf1N1B4_4450 at 4910.241 kb on + strand, within Pf1N1B4_4450 at 4910.242 kb on - strand, within Pf1N1B4_4450 at 4910.242 kb on - strand at 4910.397 kb on + strand, within Pf1N1B4_4450 at 4910.488 kb on - strand, within Pf1N1B4_4450 at 4910.493 kb on + strand, within Pf1N1B4_4450
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 4,907,215 - Pf1N1B4_4447 0.60 +0.2 4,907,446 - -0.4 4,907,447 + +1.9 4,907,447 + -1.8 4,907,448 - +1.9 4,907,448 - -1.4 4,907,448 - -1.7 4,907,491 + -0.1 4,907,491 + -0.3 4,907,491 + +0.2 4,907,491 + -1.2 4,907,491 + -0.1 4,907,492 - -0.4 4,907,492 - -1.1 4,907,496 + -0.5 4,907,497 - +0.1 4,907,635 + Pf1N1B4_4448 0.38 +0.9 4,907,635 + Pf1N1B4_4448 0.38 -1.9 4,907,636 - -0.7 4,907,636 - Pf1N1B4_4448 0.38 -0.4 4,907,701 + +0.9 4,907,719 + Pf1N1B4_4448 0.61 -1.9 4,907,719 + Pf1N1B4_4448 0.61 +0.9 4,907,770 + Pf1N1B4_4448 0.75 -0.5 4,907,863 - +0.0 4,908,143 - -0.1 4,908,407 - -1.7 4,908,475 + Pf1N1B4_4449 0.28 -1.7 4,908,838 + Pf1N1B4_4449 0.50 +1.1 4,908,839 - Pf1N1B4_4449 0.50 +0.9 4,908,839 - Pf1N1B4_4449 0.50 -0.1 4,908,839 - Pf1N1B4_4449 0.50 +0.1 4,909,097 - Pf1N1B4_4449 0.66 -0.3 4,909,207 + Pf1N1B4_4449 0.73 -1.7 4,909,207 + +0.9 4,909,208 - Pf1N1B4_4449 0.73 -0.1 4,909,304 - Pf1N1B4_4449 0.79 -0.4 4,909,369 + Pf1N1B4_4449 0.83 -0.7 4,909,528 + -2.1 4,909,729 - -1.1 4,909,729 - +1.2 4,909,740 + +0.9 4,909,740 + -1.1 4,909,740 + -0.1 4,909,741 - -0.3 4,909,741 - -1.3 4,909,754 + -0.1 4,909,755 - -0.1 4,909,830 + +0.1 4,909,830 + +1.5 4,909,830 + -0.3 4,909,936 + +0.9 4,909,937 - +1.1 4,910,140 - Pf1N1B4_4450 0.19 +0.6 4,910,213 + Pf1N1B4_4450 0.23 -1.7 4,910,241 + Pf1N1B4_4450 0.25 -0.9 4,910,241 + Pf1N1B4_4450 0.25 +1.5 4,910,242 - Pf1N1B4_4450 0.25 -0.3 4,910,242 - +2.5 4,910,397 + Pf1N1B4_4450 0.34 +1.0 4,910,488 - Pf1N1B4_4450 0.40 +0.1 4,910,493 + Pf1N1B4_4450 0.40 +0.1
Or see this region's nucleotide sequence