Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4263 and Pf1N1B4_4264 are separated by 448 nucleotides Pf1N1B4_4264 and Pf1N1B4_4265 are separated by 224 nucleotides Pf1N1B4_4265 and Pf1N1B4_4266 are separated by 154 nucleotides
Pf1N1B4_4263 - hypothetical protein, at 4,726,839 to 4,727,135
_4263
Pf1N1B4_4264 - Aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 4,727,584 to 4,728,783
_4264
Pf1N1B4_4265 - hypothetical protein, at 4,729,008 to 4,729,514
_4265
Pf1N1B4_4266 - sensor histidine kinase, at 4,729,669 to 4,731,063
_4266
Position (kb)
4727
4728
4729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4726.592 kb on - strand at 4726.608 kb on - strand at 4726.643 kb on + strand at 4726.643 kb on + strand at 4726.664 kb on + strand at 4727.244 kb on - strand at 4727.262 kb on - strand at 4727.290 kb on - strand at 4727.290 kb on - strand at 4727.430 kb on + strand at 4727.453 kb on + strand at 4727.453 kb on + strand at 4727.583 kb on + strand at 4727.583 kb on + strand at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.831 kb on - strand, within Pf1N1B4_4264 at 4727.831 kb on - strand, within Pf1N1B4_4264 at 4728.530 kb on + strand at 4728.530 kb on + strand, within Pf1N1B4_4264 at 4728.530 kb on + strand, within Pf1N1B4_4264 at 4728.531 kb on - strand at 4728.538 kb on + strand, within Pf1N1B4_4264 at 4728.595 kb on + strand, within Pf1N1B4_4264 at 4728.758 kb on + strand at 4728.758 kb on + strand at 4728.761 kb on + strand at 4728.842 kb on - strand at 4728.842 kb on - strand at 4728.908 kb on + strand at 4728.908 kb on + strand at 4728.908 kb on + strand at 4729.018 kb on + strand at 4729.157 kb on + strand, within Pf1N1B4_4265 at 4729.157 kb on + strand, within Pf1N1B4_4265 at 4729.157 kb on + strand, within Pf1N1B4_4265 at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.162 kb on - strand, within Pf1N1B4_4265 at 4729.162 kb on - strand, within Pf1N1B4_4265 at 4729.416 kb on + strand, within Pf1N1B4_4265 at 4729.443 kb on + strand, within Pf1N1B4_4265 at 4729.443 kb on + strand, within Pf1N1B4_4265 at 4729.444 kb on - strand, within Pf1N1B4_4265 at 4729.444 kb on - strand, within Pf1N1B4_4265 at 4729.595 kb on + strand at 4729.645 kb on - strand at 4729.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 4,726,592 - -0.7 4,726,608 - -1.2 4,726,643 + -1.1 4,726,643 + -0.2 4,726,664 + -1.3 4,727,244 - +0.6 4,727,262 - +0.3 4,727,290 - -1.1 4,727,290 - -0.3 4,727,430 + -1.7 4,727,453 + -0.1 4,727,453 + -0.2 4,727,583 + -1.0 4,727,583 + -0.9 4,727,830 + Pf1N1B4_4264 0.20 -3.2 4,727,830 + Pf1N1B4_4264 0.20 -1.5 4,727,830 + Pf1N1B4_4264 0.20 -0.7 4,727,830 + Pf1N1B4_4264 0.20 -2.1 4,727,831 - Pf1N1B4_4264 0.21 -0.5 4,727,831 - Pf1N1B4_4264 0.21 -0.1 4,728,530 + +0.9 4,728,530 + Pf1N1B4_4264 0.79 -0.7 4,728,530 + Pf1N1B4_4264 0.79 -0.3 4,728,531 - +0.9 4,728,538 + Pf1N1B4_4264 0.80 -0.7 4,728,595 + Pf1N1B4_4264 0.84 -2.2 4,728,758 + +0.0 4,728,758 + +0.9 4,728,761 + -0.4 4,728,842 - -0.4 4,728,842 - -1.8 4,728,908 + -0.7 4,728,908 + -2.0 4,728,908 + -0.2 4,729,018 + -0.3 4,729,157 + Pf1N1B4_4265 0.29 -2.1 4,729,157 + Pf1N1B4_4265 0.29 -0.2 4,729,157 + Pf1N1B4_4265 0.29 +0.9 4,729,158 - Pf1N1B4_4265 0.30 -0.8 4,729,158 - Pf1N1B4_4265 0.30 -0.5 4,729,158 - -0.1 4,729,158 - Pf1N1B4_4265 0.30 +0.5 4,729,158 - Pf1N1B4_4265 0.30 +1.3 4,729,158 - +1.9 4,729,161 + Pf1N1B4_4265 0.30 -1.2 4,729,161 + Pf1N1B4_4265 0.30 +0.9 4,729,161 + Pf1N1B4_4265 0.30 -0.3 4,729,161 + +0.9 4,729,161 + Pf1N1B4_4265 0.30 +0.9 4,729,161 + Pf1N1B4_4265 0.30 +1.3 4,729,161 + Pf1N1B4_4265 0.30 +0.9 4,729,161 + +0.9 4,729,161 + Pf1N1B4_4265 0.30 -0.4 4,729,161 + Pf1N1B4_4265 0.30 -2.1 4,729,162 - Pf1N1B4_4265 0.30 -0.4 4,729,162 - Pf1N1B4_4265 0.30 +1.2 4,729,416 + Pf1N1B4_4265 0.80 -1.4 4,729,443 + Pf1N1B4_4265 0.86 -1.7 4,729,443 + Pf1N1B4_4265 0.86 +2.5 4,729,444 - Pf1N1B4_4265 0.86 +0.2 4,729,444 - Pf1N1B4_4265 0.86 +1.9 4,729,595 + -0.7 4,729,645 - +0.9 4,729,645 - -1.0
Or see this region's nucleotide sequence