Experiment: Cobalt chloride 0.3125 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_4025 and Pf1N1B4_4026 overlap by 4 nucleotides Pf1N1B4_4026 and Pf1N1B4_4027 overlap by 2 nucleotides Pf1N1B4_4027 and Pf1N1B4_4028 are separated by 200 nucleotides 
        Pf1N1B4_4025 - Outer membrane porin, OprD family, at 4,476,633 to 4,477,394 
        _4025 
         
        
        Pf1N1B4_4026 - Outer membrane porin, OprD family, at 4,477,391 to 4,477,894 
        _4026 
         
        
        Pf1N1B4_4027 - hypothetical protein, at 4,477,893 to 4,478,051 
        _4027 
         
        
        Pf1N1B4_4028 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 4,478,252 to 4,479,715 
        _4028 
         Position (kb) 4477 
4478 
4479 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 4476.913 kb on + strand, within Pf1N1B4_4025 at 4476.913 kb on + strand, within Pf1N1B4_4025 at 4476.913 kb on + strand, within Pf1N1B4_4025 at 4476.914 kb on - strand, within Pf1N1B4_4025 at 4476.979 kb on + strand, within Pf1N1B4_4025 at 4476.980 kb on - strand, within Pf1N1B4_4025 at 4477.162 kb on + strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.163 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.166 kb on - strand, within Pf1N1B4_4025 at 4477.228 kb on + strand, within Pf1N1B4_4025 at 4477.228 kb on + strand, within Pf1N1B4_4025 at 4477.229 kb on - strand, within Pf1N1B4_4025 at 4477.229 kb on - strand, within Pf1N1B4_4025 at 4477.229 kb on - strand, within Pf1N1B4_4025 at 4477.232 kb on - strand, within Pf1N1B4_4025 at 4477.232 kb on - strand, within Pf1N1B4_4025 at 4477.232 kb on - strand, within Pf1N1B4_4025 at 4477.265 kb on - strand, within Pf1N1B4_4025 at 4477.298 kb on - strand, within Pf1N1B4_4025 at 4477.345 kb on + strand at 4477.346 kb on - strand at 4477.541 kb on - strand, within Pf1N1B4_4026 at 4477.581 kb on + strand, within Pf1N1B4_4026 at 4477.582 kb on - strand, within Pf1N1B4_4026 at 4477.582 kb on - strand, within Pf1N1B4_4026 at 4477.582 kb on - strand, within Pf1N1B4_4026 at 4477.821 kb on + strand, within Pf1N1B4_4026 at 4477.822 kb on - strand, within Pf1N1B4_4026 at 4477.823 kb on + strand at 4477.823 kb on + strand, within Pf1N1B4_4026 at 4477.823 kb on + strand, within Pf1N1B4_4026 at 4477.823 kb on + strand, within Pf1N1B4_4026 at 4477.823 kb on + strand, within Pf1N1B4_4026 at 4477.824 kb on - strand, within Pf1N1B4_4026 at 4477.824 kb on - strand, within Pf1N1B4_4026 at 4477.824 kb on - strand, within Pf1N1B4_4026 at 4477.876 kb on + strand at 4477.946 kb on - strand, within Pf1N1B4_4027 at 4477.946 kb on - strand, within Pf1N1B4_4027 at 4477.951 kb on + strand, within Pf1N1B4_4027 at 4478.095 kb on - strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.134 kb on + strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.135 kb on - strand at 4478.149 kb on + strand at 4478.150 kb on - strand at 4478.150 kb on - strand at 4478.150 kb on - strand at 4478.150 kb on - strand at 4478.150 kb on - strand at 4478.150 kb on - strand at 4478.150 kb on - strand at 4478.225 kb on - strand at 4478.413 kb on - strand, within Pf1N1B4_4028 at 4478.413 kb on - strand, within Pf1N1B4_4028 at 4478.413 kb on - strand, within Pf1N1B4_4028 at 4478.413 kb on - strand, within Pf1N1B4_4028 at 4478.712 kb on + strand, within Pf1N1B4_4028 at 4478.713 kb on - strand, within Pf1N1B4_4028  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Cobalt chloride 0.3125 mM remove 4,476,913 +  Pf1N1B4_4025 0.37  +1.4 4,476,913 +  Pf1N1B4_4025 0.37  -0.2 4,476,913 +  Pf1N1B4_4025 0.37  +1.0 4,476,914 -  Pf1N1B4_4025 0.37  +0.3 4,476,979 +  Pf1N1B4_4025 0.45  -0.1 4,476,980 -  Pf1N1B4_4025 0.46  -0.1 4,477,162 +  Pf1N1B4_4025 0.69  -0.4 4,477,163 -  Pf1N1B4_4025 0.70  -0.2 4,477,163 -  Pf1N1B4_4025 0.70  +0.0 4,477,163 -  Pf1N1B4_4025 0.70  -0.5 4,477,163 -  Pf1N1B4_4025 0.70  -0.2 4,477,163 -  Pf1N1B4_4025 0.70  +0.0 4,477,163 -  Pf1N1B4_4025 0.70  +0.4 4,477,163 -  Pf1N1B4_4025 0.70  +0.3 4,477,163 -  Pf1N1B4_4025 0.70  -0.3 4,477,163 -  Pf1N1B4_4025 0.70  +0.0 4,477,163 -  Pf1N1B4_4025 0.70  -2.2 4,477,163 -  Pf1N1B4_4025 0.70  +0.8 4,477,166 -  Pf1N1B4_4025 0.70  +0.3 4,477,166 -  Pf1N1B4_4025 0.70  +0.5 4,477,166 -  Pf1N1B4_4025 0.70  -0.3 4,477,166 -  Pf1N1B4_4025 0.70  -1.2 4,477,228 +  Pf1N1B4_4025 0.78  +0.7 4,477,228 +  Pf1N1B4_4025 0.78  -0.8 4,477,229 -  Pf1N1B4_4025 0.78  -2.0 4,477,229 -  Pf1N1B4_4025 0.78  +0.7 4,477,229 -  Pf1N1B4_4025 0.78  +0.4 4,477,232 -  Pf1N1B4_4025 0.79  +0.0 4,477,232 -  Pf1N1B4_4025 0.79  +0.3 4,477,232 -  Pf1N1B4_4025 0.79  -1.2 4,477,265 -  Pf1N1B4_4025 0.83  -0.2 4,477,298 -  Pf1N1B4_4025 0.87  -1.1 4,477,345 +  -1.6 4,477,346 -  +0.4 4,477,541 -  Pf1N1B4_4026 0.30  +1.0 4,477,581 +  Pf1N1B4_4026 0.38  -0.4 4,477,582 -  Pf1N1B4_4026 0.38  -0.3 4,477,582 -  Pf1N1B4_4026 0.38  +0.5 4,477,582 -  Pf1N1B4_4026 0.38  +0.7 4,477,821 +  Pf1N1B4_4026 0.85  +0.3 4,477,822 -  Pf1N1B4_4026 0.86  +1.0 4,477,823 +  -1.0 4,477,823 +  Pf1N1B4_4026 0.86  +0.8 4,477,823 +  Pf1N1B4_4026 0.86  -0.2 4,477,823 +  Pf1N1B4_4026 0.86  -2.4 4,477,823 +  Pf1N1B4_4026 0.86  +0.7 4,477,824 -  Pf1N1B4_4026 0.86  +0.0 4,477,824 -  Pf1N1B4_4026 0.86  -2.2 4,477,824 -  Pf1N1B4_4026 0.86  -0.7 4,477,876 +  -0.2 4,477,946 -  Pf1N1B4_4027 0.33  -0.6 4,477,946 -  Pf1N1B4_4027 0.33  -0.8 4,477,951 +  Pf1N1B4_4027 0.36  -0.8 4,478,095 -  -0.4 4,478,134 +  -0.9 4,478,134 +  -0.1 4,478,134 +  -0.2 4,478,134 +  +0.8 4,478,134 +  -0.6 4,478,134 +  +0.0 4,478,134 +  +0.2 4,478,134 +  +0.5 4,478,134 +  -0.2 4,478,134 +  +0.2 4,478,134 +  -0.3 4,478,134 +  -0.5 4,478,135 -  -0.2 4,478,135 -  +0.0 4,478,135 -  -0.6 4,478,135 -  -0.6 4,478,135 -  -1.3 4,478,135 -  +0.7 4,478,135 -  +0.4 4,478,135 -  -1.3 4,478,135 -  +1.0 4,478,135 -  -1.0 4,478,135 -  -1.0 4,478,135 -  -1.3 4,478,135 -  +0.0 4,478,135 -  -1.1 4,478,135 -  -1.0 4,478,135 -  -0.9 4,478,149 +  -0.4 4,478,150 -  -0.9 4,478,150 -  -2.3 4,478,150 -  -0.4 4,478,150 -  -0.1 4,478,150 -  -1.3 4,478,150 -  -0.6 4,478,150 -  -0.4 4,478,225 -  -0.4 4,478,413 -  Pf1N1B4_4028 0.11  -0.9 4,478,413 -  Pf1N1B4_4028 0.11  -1.1 4,478,413 -  Pf1N1B4_4028 0.11  -0.6 4,478,413 -  Pf1N1B4_4028 0.11  +2.0 4,478,712 +  Pf1N1B4_4028 0.31  -1.3 4,478,713 -  Pf1N1B4_4028 0.31  +0.7 
Or see this region's nucleotide sequence