Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3887 and Pf1N1B4_3888 are separated by 236 nucleotides Pf1N1B4_3888 and Pf1N1B4_3889 are separated by 60 nucleotides Pf1N1B4_3889 and Pf1N1B4_3890 overlap by 21 nucleotides
Pf1N1B4_3887 - CAAX amino terminal protease family protein, at 4,315,990 to 4,316,781
_3887
Pf1N1B4_3888 - Aconitate hydratase (EC 4.2.1.3), at 4,317,018 to 4,319,759
_3888
Pf1N1B4_3889 - hypothetical protein, at 4,319,820 to 4,320,002
_3889
Pf1N1B4_3890 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 4,319,982 to 4,321,055
_3890
Position (kb)
4317
4318
4319
4320 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4316.183 kb on - strand, within Pf1N1B4_3887 at 4316.243 kb on + strand, within Pf1N1B4_3887 at 4316.243 kb on + strand, within Pf1N1B4_3887 at 4316.498 kb on + strand, within Pf1N1B4_3887 at 4316.499 kb on - strand, within Pf1N1B4_3887 at 4316.527 kb on - strand, within Pf1N1B4_3887 at 4316.625 kb on - strand at 4316.717 kb on + strand at 4316.717 kb on + strand at 4316.759 kb on + strand at 4316.759 kb on + strand at 4316.760 kb on - strand at 4316.760 kb on - strand at 4316.955 kb on - strand at 4316.955 kb on - strand at 4316.956 kb on + strand at 4316.956 kb on + strand at 4316.956 kb on + strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.967 kb on - strand at 4316.967 kb on - strand at 4316.974 kb on - strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.073 kb on + strand at 4317.073 kb on + strand at 4317.074 kb on - strand at 4317.074 kb on - strand at 4317.074 kb on - strand at 4317.270 kb on + strand at 4317.270 kb on + strand at 4317.270 kb on + strand at 4317.270 kb on + strand at 4317.271 kb on - strand at 4317.388 kb on + strand, within Pf1N1B4_3888 at 4317.389 kb on - strand, within Pf1N1B4_3888 at 4317.451 kb on + strand, within Pf1N1B4_3888 at 4317.451 kb on + strand, within Pf1N1B4_3888 at 4317.452 kb on - strand, within Pf1N1B4_3888 at 4317.479 kb on + strand, within Pf1N1B4_3888 at 4317.479 kb on + strand, within Pf1N1B4_3888 at 4317.479 kb on + strand at 4317.479 kb on + strand, within Pf1N1B4_3888 at 4317.481 kb on + strand, within Pf1N1B4_3888 at 4317.482 kb on - strand at 4317.482 kb on - strand at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.786 kb on - strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.788 kb on - strand at 4317.788 kb on - strand at 4317.788 kb on - strand, within Pf1N1B4_3888 at 4317.788 kb on - strand at 4317.788 kb on - strand, within Pf1N1B4_3888 at 4317.788 kb on - strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.812 kb on - strand at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.945 kb on - strand, within Pf1N1B4_3888 at 4317.945 kb on - strand, within Pf1N1B4_3888 at 4317.945 kb on - strand, within Pf1N1B4_3888 at 4317.956 kb on - strand, within Pf1N1B4_3888 at 4318.240 kb on + strand, within Pf1N1B4_3888 at 4318.241 kb on - strand, within Pf1N1B4_3888 at 4318.264 kb on + strand, within Pf1N1B4_3888 at 4318.264 kb on + strand, within Pf1N1B4_3888 at 4318.264 kb on + strand, within Pf1N1B4_3888 at 4318.264 kb on + strand at 4318.267 kb on + strand, within Pf1N1B4_3888 at 4318.267 kb on + strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.444 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.730 kb on + strand, within Pf1N1B4_3888 at 4318.730 kb on + strand, within Pf1N1B4_3888 at 4318.730 kb on + strand at 4318.731 kb on - strand, within Pf1N1B4_3888 at 4318.731 kb on - strand, within Pf1N1B4_3888 at 4318.732 kb on + strand, within Pf1N1B4_3888 at 4318.733 kb on - strand, within Pf1N1B4_3888 at 4318.733 kb on - strand at 4318.733 kb on - strand, within Pf1N1B4_3888 at 4318.784 kb on - strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.259 kb on - strand, within Pf1N1B4_3888 at 4319.353 kb on + strand at 4319.353 kb on + strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.502 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.702 kb on - strand at 4319.721 kb on + strand at 4319.722 kb on - strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.775 kb on - strand at 4319.816 kb on - strand at 4319.816 kb on - strand at 4319.831 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.834 kb on - strand at 4319.847 kb on + strand, within Pf1N1B4_3889 at 4319.859 kb on - strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 4,316,183 - Pf1N1B4_3887 0.24 -2.4 4,316,243 + Pf1N1B4_3887 0.32 -0.4 4,316,243 + Pf1N1B4_3887 0.32 +0.1 4,316,498 + Pf1N1B4_3887 0.64 -1.9 4,316,499 - Pf1N1B4_3887 0.64 +0.7 4,316,527 - Pf1N1B4_3887 0.68 +0.2 4,316,625 - +0.5 4,316,717 + -0.9 4,316,717 + +0.2 4,316,759 + -1.4 4,316,759 + +0.5 4,316,760 - +1.1 4,316,760 - -0.5 4,316,955 - -1.0 4,316,955 - -0.5 4,316,956 + -1.7 4,316,956 + -0.0 4,316,956 + +0.1 4,316,957 - +0.5 4,316,957 - -1.7 4,316,957 - -0.7 4,316,957 - -0.3 4,316,957 - +0.7 4,316,957 - +0.9 4,316,957 - +0.2 4,316,957 - -0.3 4,316,957 - +1.7 4,316,957 - -0.8 4,316,964 + -0.2 4,316,964 + -0.5 4,316,964 + -0.9 4,316,964 + +0.7 4,316,964 + +0.3 4,316,964 + -0.3 4,316,964 + +0.1 4,316,964 + -0.1 4,316,965 - +1.9 4,316,965 - +0.1 4,316,965 - +0.1 4,316,965 - +0.5 4,316,965 - -0.2 4,316,965 - -0.2 4,316,965 - +0.3 4,316,965 - -1.3 4,316,965 - -0.2 4,316,965 - -0.8 4,316,965 - +0.0 4,316,965 - -0.2 4,316,965 - -1.0 4,316,965 - +0.5 4,316,965 - -0.0 4,316,965 - -0.1 4,316,967 - +0.8 4,316,967 - +0.2 4,316,974 - +0.7 4,317,046 + +1.5 4,317,046 + +1.1 4,317,046 + -0.1 4,317,046 + +0.2 4,317,046 + +3.4 4,317,047 - +0.2 4,317,047 - -2.6 4,317,047 - -0.4 4,317,047 - -0.9 4,317,047 - -0.3 4,317,047 - +1.1 4,317,047 - +0.7 4,317,047 - +1.1 4,317,073 + -1.4 4,317,073 + +0.1 4,317,074 - +0.1 4,317,074 - -0.7 4,317,074 - -0.4 4,317,270 + -0.3 4,317,270 + -1.4 4,317,270 + +0.1 4,317,270 + +0.8 4,317,271 - +0.5 4,317,388 + Pf1N1B4_3888 0.13 -0.7 4,317,389 - Pf1N1B4_3888 0.14 +0.2 4,317,451 + Pf1N1B4_3888 0.16 -2.4 4,317,451 + Pf1N1B4_3888 0.16 -0.9 4,317,452 - Pf1N1B4_3888 0.16 +0.4 4,317,479 + Pf1N1B4_3888 0.17 +0.5 4,317,479 + Pf1N1B4_3888 0.17 -0.5 4,317,479 + +1.1 4,317,479 + Pf1N1B4_3888 0.17 +0.2 4,317,481 + Pf1N1B4_3888 0.17 -0.2 4,317,482 - -0.9 4,317,482 - -1.3 4,317,482 - Pf1N1B4_3888 0.17 +2.1 4,317,482 - Pf1N1B4_3888 0.17 -1.9 4,317,482 - Pf1N1B4_3888 0.17 -0.2 4,317,482 - Pf1N1B4_3888 0.17 -2.0 4,317,482 - Pf1N1B4_3888 0.17 -0.1 4,317,786 - Pf1N1B4_3888 0.28 +0.1 4,317,787 + Pf1N1B4_3888 0.28 +0.5 4,317,787 + Pf1N1B4_3888 0.28 +1.4 4,317,787 + Pf1N1B4_3888 0.28 +0.1 4,317,787 + -0.3 4,317,787 + Pf1N1B4_3888 0.28 -0.7 4,317,787 + Pf1N1B4_3888 0.28 -0.3 4,317,787 + Pf1N1B4_3888 0.28 -1.7 4,317,787 + Pf1N1B4_3888 0.28 -1.5 4,317,787 + Pf1N1B4_3888 0.28 -0.5 4,317,788 - -0.5 4,317,788 - -0.5 4,317,788 - Pf1N1B4_3888 0.28 -1.7 4,317,788 - -0.5 4,317,788 - Pf1N1B4_3888 0.28 -0.3 4,317,788 - Pf1N1B4_3888 0.28 -0.9 4,317,811 + Pf1N1B4_3888 0.29 -0.3 4,317,811 + Pf1N1B4_3888 0.29 -1.4 4,317,811 + Pf1N1B4_3888 0.29 +1.5 4,317,811 + Pf1N1B4_3888 0.29 -1.0 4,317,812 - Pf1N1B4_3888 0.29 +0.9 4,317,812 - Pf1N1B4_3888 0.29 +0.1 4,317,812 - Pf1N1B4_3888 0.29 -0.8 4,317,812 - -0.3 4,317,812 - Pf1N1B4_3888 0.29 +0.9 4,317,945 - Pf1N1B4_3888 0.34 +1.1 4,317,945 - Pf1N1B4_3888 0.34 -2.4 4,317,945 - Pf1N1B4_3888 0.34 +0.7 4,317,956 - Pf1N1B4_3888 0.34 +1.9 4,318,240 + Pf1N1B4_3888 0.45 -1.3 4,318,241 - Pf1N1B4_3888 0.45 -1.3 4,318,264 + Pf1N1B4_3888 0.45 +1.1 4,318,264 + Pf1N1B4_3888 0.45 +0.3 4,318,264 + Pf1N1B4_3888 0.45 +0.5 4,318,264 + +0.5 4,318,267 + Pf1N1B4_3888 0.46 -1.4 4,318,267 + Pf1N1B4_3888 0.46 -1.1 4,318,268 - Pf1N1B4_3888 0.46 -0.2 4,318,268 - Pf1N1B4_3888 0.46 +0.1 4,318,268 - Pf1N1B4_3888 0.46 -0.5 4,318,268 - Pf1N1B4_3888 0.46 +1.1 4,318,444 + Pf1N1B4_3888 0.52 +0.2 4,318,448 + Pf1N1B4_3888 0.52 -0.1 4,318,448 + Pf1N1B4_3888 0.52 +1.1 4,318,448 + Pf1N1B4_3888 0.52 -2.5 4,318,448 + Pf1N1B4_3888 0.52 +0.5 4,318,449 - Pf1N1B4_3888 0.52 +2.7 4,318,449 - Pf1N1B4_3888 0.52 +0.5 4,318,449 - Pf1N1B4_3888 0.52 -0.2 4,318,449 - Pf1N1B4_3888 0.52 -1.3 4,318,449 - Pf1N1B4_3888 0.52 +0.1 4,318,449 - Pf1N1B4_3888 0.52 -0.4 4,318,730 + Pf1N1B4_3888 0.62 -0.3 4,318,730 + Pf1N1B4_3888 0.62 +0.5 4,318,730 + -0.0 4,318,731 - Pf1N1B4_3888 0.62 +1.1 4,318,731 - Pf1N1B4_3888 0.62 +0.1 4,318,732 + Pf1N1B4_3888 0.63 -0.2 4,318,733 - Pf1N1B4_3888 0.63 +0.4 4,318,733 - -0.9 4,318,733 - Pf1N1B4_3888 0.63 +0.4 4,318,784 - Pf1N1B4_3888 0.64 +0.7 4,319,155 + Pf1N1B4_3888 0.78 +0.7 4,319,155 + Pf1N1B4_3888 0.78 +0.1 4,319,155 + Pf1N1B4_3888 0.78 -0.9 4,319,155 + Pf1N1B4_3888 0.78 -1.3 4,319,155 + Pf1N1B4_3888 0.78 +1.1 4,319,155 + Pf1N1B4_3888 0.78 -1.3 4,319,156 - Pf1N1B4_3888 0.78 -1.1 4,319,156 - Pf1N1B4_3888 0.78 -0.3 4,319,156 - Pf1N1B4_3888 0.78 -0.7 4,319,156 - Pf1N1B4_3888 0.78 +1.1 4,319,156 - Pf1N1B4_3888 0.78 +1.5 4,319,156 - Pf1N1B4_3888 0.78 -0.7 4,319,156 - Pf1N1B4_3888 0.78 +0.1 4,319,201 - Pf1N1B4_3888 0.80 +0.3 4,319,201 - Pf1N1B4_3888 0.80 +0.8 4,319,259 - Pf1N1B4_3888 0.82 +0.7 4,319,353 + +0.1 4,319,353 + Pf1N1B4_3888 0.85 +1.1 4,319,354 - Pf1N1B4_3888 0.85 +0.1 4,319,354 - Pf1N1B4_3888 0.85 -1.3 4,319,502 + +0.5 4,319,701 + -0.5 4,319,701 + -0.9 4,319,701 + -0.7 4,319,702 - -0.2 4,319,721 + +1.4 4,319,722 - -0.7 4,319,774 + +2.4 4,319,774 + -1.9 4,319,774 + -0.8 4,319,775 - +0.3 4,319,816 - -1.6 4,319,816 - +0.5 4,319,831 + -1.9 4,319,833 + -0.7 4,319,833 + +1.1 4,319,833 + +0.3 4,319,834 - -0.4 4,319,847 + Pf1N1B4_3889 0.15 -0.7 4,319,859 - Pf1N1B4_3889 0.21 +0.4 4,319,977 + Pf1N1B4_3889 0.86 +1.4 4,319,977 + Pf1N1B4_3889 0.86 -0.2 4,319,996 + -0.5
Or see this region's nucleotide sequence