Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3223 and Pf1N1B4_3224 are separated by 167 nucleotides Pf1N1B4_3224 and Pf1N1B4_3225 are separated by 230 nucleotides Pf1N1B4_3225 and Pf1N1B4_3226 are separated by 104 nucleotides Pf1N1B4_3226 and Pf1N1B4_3227 are separated by 144 nucleotides
Pf1N1B4_3223 - Major facilitator family transporter, at 3,608,035 to 3,609,384
_3223
Pf1N1B4_3224 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 3,609,552 to 3,612,398
_3224
Pf1N1B4_3225 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 3,612,629 to 3,612,970
_3225
Pf1N1B4_3226 - FIG00959308: hypothetical protein, at 3,613,075 to 3,613,236
_3226
Pf1N1B4_3227 - hypothetical protein, at 3,613,381 to 3,613,497
_3227
Position (kb)
3609
3610
3611
3612
3613 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.731 kb on + strand, within Pf1N1B4_3223 at 3608.732 kb on - strand, within Pf1N1B4_3223 at 3608.732 kb on - strand, within Pf1N1B4_3223 at 3608.732 kb on - strand at 3608.941 kb on - strand, within Pf1N1B4_3223 at 3609.019 kb on + strand, within Pf1N1B4_3223 at 3609.019 kb on + strand, within Pf1N1B4_3223 at 3609.019 kb on + strand, within Pf1N1B4_3223 at 3609.020 kb on - strand, within Pf1N1B4_3223 at 3609.020 kb on - strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.028 kb on + strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.029 kb on - strand, within Pf1N1B4_3223 at 3609.030 kb on + strand, within Pf1N1B4_3223 at 3609.030 kb on + strand, within Pf1N1B4_3223 at 3609.030 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.040 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.112 kb on + strand, within Pf1N1B4_3223 at 3609.113 kb on - strand, within Pf1N1B4_3223 at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.247 kb on + strand at 3609.247 kb on + strand, within Pf1N1B4_3223 at 3609.248 kb on - strand, within Pf1N1B4_3223 at 3609.251 kb on + strand at 3609.251 kb on + strand at 3609.251 kb on + strand at 3609.252 kb on - strand at 3609.252 kb on - strand at 3609.252 kb on - strand at 3609.274 kb on - strand at 3609.311 kb on + strand at 3609.311 kb on + strand at 3609.311 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.420 kb on + strand at 3609.421 kb on - strand at 3609.421 kb on - strand at 3609.433 kb on + strand at 3609.434 kb on - strand at 3609.532 kb on - strand at 3609.532 kb on - strand at 3609.949 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.994 kb on + strand, within Pf1N1B4_3224 at 3609.995 kb on - strand, within Pf1N1B4_3224 at 3609.995 kb on - strand, within Pf1N1B4_3224 at 3610.165 kb on + strand, within Pf1N1B4_3224 at 3610.166 kb on - strand, within Pf1N1B4_3224 at 3610.166 kb on - strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.300 kb on + strand, within Pf1N1B4_3224 at 3610.301 kb on - strand, within Pf1N1B4_3224 at 3610.301 kb on - strand, within Pf1N1B4_3224 at 3610.301 kb on - strand, within Pf1N1B4_3224 at 3610.586 kb on - strand, within Pf1N1B4_3224 at 3610.586 kb on - strand, within Pf1N1B4_3224 at 3610.804 kb on + strand, within Pf1N1B4_3224 at 3610.804 kb on + strand, within Pf1N1B4_3224 at 3611.086 kb on + strand, within Pf1N1B4_3224 at 3611.087 kb on - strand, within Pf1N1B4_3224 at 3611.359 kb on + strand, within Pf1N1B4_3224 at 3611.519 kb on - strand, within Pf1N1B4_3224 at 3611.791 kb on - strand, within Pf1N1B4_3224 at 3611.791 kb on - strand, within Pf1N1B4_3224 at 3611.791 kb on - strand, within Pf1N1B4_3224 at 3611.829 kb on + strand, within Pf1N1B4_3224 at 3612.146 kb on - strand at 3612.146 kb on - strand at 3612.292 kb on + strand at 3612.431 kb on - strand at 3612.465 kb on + strand at 3612.465 kb on + strand at 3612.465 kb on + strand at 3612.465 kb on + strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.466 kb on - strand at 3612.540 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.621 kb on + strand at 3612.622 kb on - strand at 3612.622 kb on - strand at 3612.622 kb on - strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.625 kb on + strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.626 kb on - strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.628 kb on + strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.629 kb on - strand at 3612.660 kb on + strand at 3613.011 kb on - strand at 3613.011 kb on - strand at 3613.138 kb on - strand at 3613.161 kb on - strand at 3613.161 kb on - strand, within Pf1N1B4_3226 at 3613.268 kb on - strand at 3613.282 kb on + strand at 3613.339 kb on - strand at 3613.343 kb on + strand at 3613.343 kb on + strand at 3613.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 3,608,731 + Pf1N1B4_3223 0.52 +0.1 3,608,731 + Pf1N1B4_3223 0.52 -1.5 3,608,731 + Pf1N1B4_3223 0.52 +0.6 3,608,731 + Pf1N1B4_3223 0.52 -0.9 3,608,731 + Pf1N1B4_3223 0.52 +1.1 3,608,731 + Pf1N1B4_3223 0.52 +0.2 3,608,732 - Pf1N1B4_3223 0.52 -0.2 3,608,732 - Pf1N1B4_3223 0.52 -0.7 3,608,732 - -0.7 3,608,941 - Pf1N1B4_3223 0.67 +1.1 3,609,019 + Pf1N1B4_3223 0.73 -1.8 3,609,019 + Pf1N1B4_3223 0.73 +0.2 3,609,019 + Pf1N1B4_3223 0.73 -0.1 3,609,020 - Pf1N1B4_3223 0.73 +0.5 3,609,020 - Pf1N1B4_3223 0.73 -0.5 3,609,028 + Pf1N1B4_3223 0.74 -0.7 3,609,028 + Pf1N1B4_3223 0.74 -0.5 3,609,028 + Pf1N1B4_3223 0.74 -0.2 3,609,028 + Pf1N1B4_3223 0.74 -1.5 3,609,028 + Pf1N1B4_3223 0.74 +0.8 3,609,028 + Pf1N1B4_3223 0.74 -1.4 3,609,028 + Pf1N1B4_3223 0.74 +1.3 3,609,028 + Pf1N1B4_3223 0.74 -0.7 3,609,029 - Pf1N1B4_3223 0.74 +0.9 3,609,029 - Pf1N1B4_3223 0.74 +2.9 3,609,029 - Pf1N1B4_3223 0.74 -0.0 3,609,029 - Pf1N1B4_3223 0.74 +1.9 3,609,030 + Pf1N1B4_3223 0.74 -1.5 3,609,030 + Pf1N1B4_3223 0.74 -0.5 3,609,030 + Pf1N1B4_3223 0.74 -0.0 3,609,040 + Pf1N1B4_3223 0.74 -1.7 3,609,040 + Pf1N1B4_3223 0.74 +0.9 3,609,040 + Pf1N1B4_3223 0.74 +0.6 3,609,040 + Pf1N1B4_3223 0.74 -0.9 3,609,112 + Pf1N1B4_3223 0.80 -0.5 3,609,112 + Pf1N1B4_3223 0.80 -1.7 3,609,112 + Pf1N1B4_3223 0.80 +0.9 3,609,112 + Pf1N1B4_3223 0.80 +2.3 3,609,113 - Pf1N1B4_3223 0.80 +1.7 3,609,247 + Pf1N1B4_3223 0.90 +1.3 3,609,247 + Pf1N1B4_3223 0.90 +0.7 3,609,247 + +0.8 3,609,247 + Pf1N1B4_3223 0.90 -1.8 3,609,247 + Pf1N1B4_3223 0.90 -0.2 3,609,247 + +1.3 3,609,247 + Pf1N1B4_3223 0.90 -2.0 3,609,248 - Pf1N1B4_3223 0.90 -0.1 3,609,251 + +3.2 3,609,251 + -0.2 3,609,251 + -1.2 3,609,252 - +0.6 3,609,252 - +1.0 3,609,252 - -0.8 3,609,274 - -1.5 3,609,311 + -1.0 3,609,311 + +1.6 3,609,311 + -0.5 3,609,420 + +0.0 3,609,420 + +1.8 3,609,420 + -1.7 3,609,420 + -0.7 3,609,420 + +1.3 3,609,420 + -0.7 3,609,421 - +0.5 3,609,421 - -0.7 3,609,433 + +1.8 3,609,434 - +0.4 3,609,532 - +0.7 3,609,532 - +0.3 3,609,949 + Pf1N1B4_3224 0.14 -1.0 3,609,994 + Pf1N1B4_3224 0.16 +0.2 3,609,994 + Pf1N1B4_3224 0.16 -0.7 3,609,994 + Pf1N1B4_3224 0.16 +0.9 3,609,994 + Pf1N1B4_3224 0.16 -1.5 3,609,994 + Pf1N1B4_3224 0.16 +0.3 3,609,995 - Pf1N1B4_3224 0.16 -0.2 3,609,995 - Pf1N1B4_3224 0.16 +0.5 3,610,165 + Pf1N1B4_3224 0.22 -1.6 3,610,166 - Pf1N1B4_3224 0.22 +0.8 3,610,166 - Pf1N1B4_3224 0.22 +0.1 3,610,300 + Pf1N1B4_3224 0.26 -1.0 3,610,300 + Pf1N1B4_3224 0.26 +0.9 3,610,300 + Pf1N1B4_3224 0.26 +2.7 3,610,300 + Pf1N1B4_3224 0.26 +0.6 3,610,300 + Pf1N1B4_3224 0.26 +0.3 3,610,300 + Pf1N1B4_3224 0.26 +0.4 3,610,301 - Pf1N1B4_3224 0.26 +0.8 3,610,301 - Pf1N1B4_3224 0.26 -0.5 3,610,301 - Pf1N1B4_3224 0.26 +0.3 3,610,586 - Pf1N1B4_3224 0.36 +1.6 3,610,586 - Pf1N1B4_3224 0.36 -0.2 3,610,804 + Pf1N1B4_3224 0.44 +0.8 3,610,804 + Pf1N1B4_3224 0.44 -0.2 3,611,086 + Pf1N1B4_3224 0.54 +0.7 3,611,087 - Pf1N1B4_3224 0.54 +1.9 3,611,359 + Pf1N1B4_3224 0.63 +1.5 3,611,519 - Pf1N1B4_3224 0.69 +0.9 3,611,791 - Pf1N1B4_3224 0.79 +0.1 3,611,791 - Pf1N1B4_3224 0.79 +2.0 3,611,791 - Pf1N1B4_3224 0.79 +1.8 3,611,829 + Pf1N1B4_3224 0.80 +1.2 3,612,146 - +1.6 3,612,146 - -0.1 3,612,292 + -2.2 3,612,431 - +1.0 3,612,465 + +2.6 3,612,465 + +0.5 3,612,465 + +0.2 3,612,465 + -0.7 3,612,466 - -2.6 3,612,466 - +0.4 3,612,466 - +1.1 3,612,466 - -1.2 3,612,466 - +0.3 3,612,466 - -0.2 3,612,466 - -0.2 3,612,466 - +0.9 3,612,540 + -0.9 3,612,621 + +0.9 3,612,621 + +1.3 3,612,621 + +1.5 3,612,621 + +1.3 3,612,621 + -0.2 3,612,622 - -0.3 3,612,622 - +0.3 3,612,622 - -1.5 3,612,625 + -0.7 3,612,625 + -1.0 3,612,625 + -0.0 3,612,625 + -0.9 3,612,625 + -0.4 3,612,625 + -1.5 3,612,625 + -0.1 3,612,625 + +0.7 3,612,625 + -0.7 3,612,626 - +0.6 3,612,626 - -0.4 3,612,626 - -1.8 3,612,626 - +2.1 3,612,626 - -0.5 3,612,626 - +1.9 3,612,626 - -0.2 3,612,626 - -0.5 3,612,626 - +0.0 3,612,626 - -0.7 3,612,626 - +0.3 3,612,626 - +0.6 3,612,626 - +0.2 3,612,626 - -0.1 3,612,626 - -2.2 3,612,626 - +0.3 3,612,626 - +0.3 3,612,626 - -1.2 3,612,626 - +0.3 3,612,628 + -0.8 3,612,628 + +1.1 3,612,628 + +0.3 3,612,628 + +0.7 3,612,628 + +1.9 3,612,628 + +2.5 3,612,628 + +0.2 3,612,628 + +0.8 3,612,628 + +0.8 3,612,628 + +0.4 3,612,628 + -2.0 3,612,628 + -0.4 3,612,629 - +0.1 3,612,629 - +0.3 3,612,629 - -1.1 3,612,629 - +0.5 3,612,629 - -0.2 3,612,629 - -1.0 3,612,629 - +1.1 3,612,629 - +2.3 3,612,629 - -0.7 3,612,629 - -0.3 3,612,629 - +1.3 3,612,629 - +1.1 3,612,629 - -0.3 3,612,629 - +0.4 3,612,629 - +0.1 3,612,629 - -1.7 3,612,629 - -1.7 3,612,629 - +0.0 3,612,660 + -0.5 3,613,011 - -0.9 3,613,011 - +0.1 3,613,138 - -0.2 3,613,161 - +1.3 3,613,161 - Pf1N1B4_3226 0.53 +1.6 3,613,268 - -0.2 3,613,282 + +0.8 3,613,339 - -0.2 3,613,343 + +0.2 3,613,343 + +0.3 3,613,344 - -0.1
Or see this region's nucleotide sequence