Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_301 and Pf1N1B4_302 are separated by 259 nucleotides Pf1N1B4_302 and Pf1N1B4_303 are separated by 105 nucleotides Pf1N1B4_303 and Pf1N1B4_304 are separated by 234 nucleotides
Pf1N1B4_301 - FIG00957636: hypothetical protein, at 354,986 to 355,294
_301
Pf1N1B4_302 - Membrane protein, distant similarity to thiosulphate:quinone oxidoreductase DoxD, at 355,554 to 355,988
_302
Pf1N1B4_303 - Transglycosylase, Slt family, at 356,094 to 357,515
_303
Pf1N1B4_304 - Deoxyribonuclease TatD, at 357,750 to 358,559
_304
Position (kb) 356
357
358 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 355.185 kb on + strand, within Pf1N1B4_301 at 355.186 kb on - strand, within Pf1N1B4_301 at 355.271 kb on + strand at 355.271 kb on + strand at 355.271 kb on + strand at 355.272 kb on - strand at 355.272 kb on - strand at 355.272 kb on - strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.283 kb on + strand at 355.284 kb on - strand at 355.364 kb on + strand at 355.390 kb on - strand at 355.426 kb on + strand at 355.426 kb on + strand at 355.427 kb on - strand at 355.458 kb on + strand at 355.458 kb on + strand at 355.458 kb on + strand at 355.646 kb on - strand, within Pf1N1B4_302 at 355.655 kb on + strand, within Pf1N1B4_302 at 355.694 kb on + strand, within Pf1N1B4_302 at 355.695 kb on - strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.837 kb on + strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.838 kb on - strand, within Pf1N1B4_302 at 355.926 kb on - strand, within Pf1N1B4_302 at 355.965 kb on + strand at 355.965 kb on + strand at 355.965 kb on + strand at 355.965 kb on + strand at 355.965 kb on + strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 355.966 kb on - strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.161 kb on + strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.162 kb on - strand at 356.164 kb on + strand at 356.164 kb on + strand at 356.164 kb on + strand at 356.164 kb on + strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.165 kb on - strand at 356.177 kb on + strand at 356.177 kb on + strand at 356.180 kb on + strand at 356.180 kb on + strand at 356.180 kb on + strand at 356.180 kb on + strand at 356.181 kb on - strand at 356.181 kb on - strand at 356.181 kb on - strand at 356.181 kb on - strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.185 kb on + strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.186 kb on - strand at 356.314 kb on + strand, within Pf1N1B4_303 at 356.314 kb on + strand, within Pf1N1B4_303 at 356.314 kb on + strand, within Pf1N1B4_303 at 356.314 kb on + strand, within Pf1N1B4_303 at 356.315 kb on - strand, within Pf1N1B4_303 at 356.395 kb on + strand, within Pf1N1B4_303 at 356.395 kb on + strand, within Pf1N1B4_303 at 356.396 kb on - strand, within Pf1N1B4_303 at 356.435 kb on + strand, within Pf1N1B4_303 at 356.438 kb on + strand, within Pf1N1B4_303 at 356.438 kb on + strand, within Pf1N1B4_303 at 356.439 kb on - strand, within Pf1N1B4_303 at 356.439 kb on - strand, within Pf1N1B4_303 at 356.439 kb on - strand, within Pf1N1B4_303 at 356.595 kb on + strand, within Pf1N1B4_303 at 356.657 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.711 kb on - strand, within Pf1N1B4_303 at 356.773 kb on + strand, within Pf1N1B4_303 at 356.773 kb on + strand, within Pf1N1B4_303 at 357.061 kb on + strand, within Pf1N1B4_303 at 357.125 kb on + strand at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.156 kb on + strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.157 kb on - strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.277 kb on + strand, within Pf1N1B4_303 at 357.278 kb on - strand at 357.304 kb on + strand, within Pf1N1B4_303 at 357.598 kb on + strand at 357.671 kb on + strand at 357.681 kb on + strand at 357.681 kb on + strand at 357.681 kb on + strand at 357.698 kb on + strand at 357.700 kb on + strand at 357.700 kb on + strand at 357.706 kb on + strand at 357.717 kb on + strand at 357.718 kb on - strand at 357.743 kb on + strand at 357.744 kb on - strand at 357.744 kb on - strand at 357.744 kb on - strand at 357.828 kb on + strand at 357.829 kb on - strand at 358.347 kb on - strand, within Pf1N1B4_304 at 358.384 kb on - strand, within Pf1N1B4_304 at 358.384 kb on - strand, within Pf1N1B4_304 at 358.441 kb on - strand, within Pf1N1B4_304
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 355,185 + Pf1N1B4_301 0.64 -0.1 355,186 - Pf1N1B4_301 0.65 -0.8 355,271 + -0.6 355,271 + -0.6 355,271 + +0.0 355,272 - -1.1 355,272 - -0.6 355,272 - +0.7 355,283 + +0.4 355,283 + -0.3 355,283 + +0.5 355,283 + -0.1 355,284 - +1.9 355,364 + -0.8 355,390 - -0.4 355,426 + -1.0 355,426 + +0.5 355,427 - -1.6 355,458 + -0.7 355,458 + +0.6 355,458 + +1.0 355,646 - Pf1N1B4_302 0.21 -1.0 355,655 + Pf1N1B4_302 0.23 +2.2 355,694 + Pf1N1B4_302 0.32 -0.0 355,695 - Pf1N1B4_302 0.32 -0.7 355,837 + Pf1N1B4_302 0.65 -0.3 355,837 + Pf1N1B4_302 0.65 -0.6 355,837 + Pf1N1B4_302 0.65 -0.4 355,837 + Pf1N1B4_302 0.65 +1.2 355,838 - Pf1N1B4_302 0.65 -0.1 355,838 - Pf1N1B4_302 0.65 -0.7 355,838 - Pf1N1B4_302 0.65 -0.6 355,838 - Pf1N1B4_302 0.65 -2.7 355,838 - Pf1N1B4_302 0.65 -0.4 355,838 - Pf1N1B4_302 0.65 -0.7 355,838 - Pf1N1B4_302 0.65 -0.0 355,926 - Pf1N1B4_302 0.86 -0.2 355,965 + +1.0 355,965 + -0.6 355,965 + -0.5 355,965 + -0.1 355,965 + +0.2 355,966 - +0.2 355,966 - -0.1 355,966 - -0.7 355,966 - +4.2 355,966 - -1.4 355,966 - +0.6 356,161 + -1.1 356,161 + -0.4 356,161 + -1.0 356,161 + -0.8 356,161 + +1.2 356,162 - +0.2 356,162 - -0.1 356,162 - -0.0 356,162 - +0.1 356,162 - -1.1 356,162 - -0.4 356,162 - -1.4 356,162 - +1.0 356,164 + -0.1 356,164 + -1.8 356,164 + +1.2 356,164 + +0.0 356,165 - +0.3 356,165 - +0.4 356,165 - -0.0 356,165 - +2.4 356,165 - +0.3 356,165 - +0.6 356,165 - -0.4 356,165 - -0.1 356,165 - -0.1 356,165 - -0.7 356,165 - -0.6 356,165 - +1.2 356,165 - -0.8 356,165 - +0.2 356,165 - +0.9 356,165 - +1.0 356,165 - +0.5 356,177 + -1.8 356,177 + +0.6 356,180 + -0.7 356,180 + -0.1 356,180 + +1.8 356,180 + -0.7 356,181 - +1.4 356,181 - -0.6 356,181 - +1.2 356,181 - +0.0 356,185 + -0.8 356,185 + -0.1 356,185 + -0.7 356,185 + -0.4 356,185 + -2.2 356,185 + -2.9 356,185 + -1.1 356,185 + -0.8 356,186 - +0.2 356,186 - +1.9 356,186 - -0.1 356,186 - +0.6 356,186 - +2.0 356,186 - -1.1 356,186 - +0.6 356,186 - +0.6 356,314 + Pf1N1B4_303 0.15 +0.8 356,314 + Pf1N1B4_303 0.15 +1.6 356,314 + Pf1N1B4_303 0.15 +1.0 356,314 + Pf1N1B4_303 0.15 -0.9 356,315 - Pf1N1B4_303 0.16 +0.7 356,395 + Pf1N1B4_303 0.21 +0.3 356,395 + Pf1N1B4_303 0.21 +0.1 356,396 - Pf1N1B4_303 0.21 -1.4 356,435 + Pf1N1B4_303 0.24 +0.0 356,438 + Pf1N1B4_303 0.24 -0.2 356,438 + Pf1N1B4_303 0.24 -0.8 356,439 - Pf1N1B4_303 0.24 +0.9 356,439 - Pf1N1B4_303 0.24 +0.6 356,439 - Pf1N1B4_303 0.24 +0.2 356,595 + Pf1N1B4_303 0.35 -0.2 356,657 - Pf1N1B4_303 0.40 -1.0 356,711 - Pf1N1B4_303 0.43 +0.7 356,711 - Pf1N1B4_303 0.43 -0.4 356,711 - Pf1N1B4_303 0.43 +1.2 356,711 - Pf1N1B4_303 0.43 +0.4 356,711 - Pf1N1B4_303 0.43 -2.1 356,773 + Pf1N1B4_303 0.48 +0.8 356,773 + Pf1N1B4_303 0.48 -0.4 357,061 + Pf1N1B4_303 0.68 -0.6 357,125 + +0.6 357,156 + Pf1N1B4_303 0.75 +0.2 357,156 + Pf1N1B4_303 0.75 +0.8 357,156 + Pf1N1B4_303 0.75 -0.1 357,156 + Pf1N1B4_303 0.75 +0.9 357,156 + Pf1N1B4_303 0.75 +0.9 357,156 + Pf1N1B4_303 0.75 +0.6 357,157 - Pf1N1B4_303 0.75 +0.8 357,157 - Pf1N1B4_303 0.75 +0.9 357,157 - Pf1N1B4_303 0.75 -0.3 357,157 - Pf1N1B4_303 0.75 +0.6 357,157 - Pf1N1B4_303 0.75 +1.9 357,277 + Pf1N1B4_303 0.83 -0.8 357,277 + Pf1N1B4_303 0.83 -0.6 357,277 + Pf1N1B4_303 0.83 +1.2 357,277 + Pf1N1B4_303 0.83 +0.8 357,277 + Pf1N1B4_303 0.83 +1.2 357,277 + Pf1N1B4_303 0.83 -1.6 357,277 + Pf1N1B4_303 0.83 -0.1 357,277 + Pf1N1B4_303 0.83 +0.4 357,278 - -0.4 357,304 + Pf1N1B4_303 0.85 +2.2 357,598 + -1.1 357,671 + -0.7 357,681 + -0.2 357,681 + +0.6 357,681 + +0.2 357,698 + +0.4 357,700 + -0.9 357,700 + -0.3 357,706 + -1.4 357,717 + -3.6 357,718 - +0.2 357,743 + +2.4 357,744 - +1.8 357,744 - +0.4 357,744 - +0.0 357,828 + +0.0 357,829 - +0.5 358,347 - Pf1N1B4_304 0.74 -0.3 358,384 - Pf1N1B4_304 0.78 +0.4 358,384 - Pf1N1B4_304 0.78 +1.1 358,441 - Pf1N1B4_304 0.85 -0.2
Or see this region's nucleotide sequence