Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotides Pf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotides Pf1N1B4_2741 and Pf1N1B4_2742 are separated by 141 nucleotides
Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835
_2739
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400
_2741
Pf1N1B4_2742 - putative exported protein, at 3,080,542 to 3,081,501
_2742
Position (kb)
3079
3080
3081 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3078.833 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.879 kb on + strand at 3079.057 kb on - strand, within Pf1N1B4_2740 at 3079.127 kb on + strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.166 kb on + strand, within Pf1N1B4_2740 at 3079.268 kb on + strand, within Pf1N1B4_2740 at 3079.311 kb on + strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.312 kb on - strand, within Pf1N1B4_2740 at 3079.445 kb on + strand, within Pf1N1B4_2740 at 3079.445 kb on + strand, within Pf1N1B4_2740 at 3079.446 kb on - strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.475 kb on + strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.476 kb on - strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.529 kb on + strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.530 kb on - strand, within Pf1N1B4_2740 at 3079.680 kb on - strand, within Pf1N1B4_2740 at 3080.118 kb on - strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.478 kb on + strand at 3080.580 kb on + strand at 3080.581 kb on - strand at 3080.727 kb on + strand, within Pf1N1B4_2742 at 3080.770 kb on + strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.835 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3081.136 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.308 kb on + strand, within Pf1N1B4_2742 at 3081.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 3,078,833 + +1.6 3,078,851 + +0.3 3,078,851 + -0.4 3,078,851 + +1.2 3,078,851 + -0.2 3,078,852 - +1.0 3,078,852 - -0.6 3,078,852 - +0.2 3,078,852 - -1.9 3,078,852 - -1.2 3,078,879 + +1.2 3,079,057 - Pf1N1B4_2740 0.13 -0.5 3,079,127 + Pf1N1B4_2740 0.21 -0.4 3,079,128 - Pf1N1B4_2740 0.21 -0.8 3,079,128 - Pf1N1B4_2740 0.21 -0.4 3,079,166 + Pf1N1B4_2740 0.26 -0.3 3,079,268 + Pf1N1B4_2740 0.38 -0.3 3,079,311 + Pf1N1B4_2740 0.43 -0.2 3,079,312 - Pf1N1B4_2740 0.43 -0.4 3,079,312 - Pf1N1B4_2740 0.43 -0.1 3,079,312 - Pf1N1B4_2740 0.43 -0.5 3,079,445 + Pf1N1B4_2740 0.58 +1.6 3,079,445 + Pf1N1B4_2740 0.58 -1.4 3,079,446 - Pf1N1B4_2740 0.58 +0.8 3,079,475 + Pf1N1B4_2740 0.61 -1.0 3,079,475 + Pf1N1B4_2740 0.61 -0.4 3,079,475 + Pf1N1B4_2740 0.61 +0.2 3,079,475 + Pf1N1B4_2740 0.61 -2.1 3,079,475 + Pf1N1B4_2740 0.61 +1.4 3,079,476 - Pf1N1B4_2740 0.61 -0.1 3,079,476 - Pf1N1B4_2740 0.61 +1.0 3,079,476 - Pf1N1B4_2740 0.61 +0.8 3,079,476 - Pf1N1B4_2740 0.61 +0.2 3,079,476 - Pf1N1B4_2740 0.61 -1.2 3,079,529 + Pf1N1B4_2740 0.68 -0.8 3,079,529 + Pf1N1B4_2740 0.68 +2.8 3,079,529 + Pf1N1B4_2740 0.68 +1.6 3,079,529 + Pf1N1B4_2740 0.68 -1.4 3,079,529 + Pf1N1B4_2740 0.68 +1.2 3,079,529 + Pf1N1B4_2740 0.68 +0.2 3,079,529 + Pf1N1B4_2740 0.68 +0.8 3,079,529 + Pf1N1B4_2740 0.68 +0.2 3,079,529 + Pf1N1B4_2740 0.68 -0.3 3,079,529 + Pf1N1B4_2740 0.68 -0.3 3,079,530 - Pf1N1B4_2740 0.68 +1.2 3,079,530 - Pf1N1B4_2740 0.68 +0.2 3,079,530 - Pf1N1B4_2740 0.68 -0.2 3,079,530 - Pf1N1B4_2740 0.68 +0.1 3,079,530 - Pf1N1B4_2740 0.68 -0.4 3,079,680 - Pf1N1B4_2740 0.85 -0.4 3,080,118 - Pf1N1B4_2741 0.52 -0.4 3,080,159 + Pf1N1B4_2741 0.59 +1.6 3,080,159 + Pf1N1B4_2741 0.59 -0.5 3,080,159 + Pf1N1B4_2741 0.59 +0.7 3,080,159 + Pf1N1B4_2741 0.59 +1.3 3,080,160 - Pf1N1B4_2741 0.59 -0.4 3,080,160 - Pf1N1B4_2741 0.59 +1.6 3,080,405 + +1.6 3,080,405 + -0.2 3,080,405 + -1.2 3,080,405 + -0.4 3,080,405 + +0.7 3,080,405 + +0.1 3,080,405 + +2.0 3,080,405 + +0.3 3,080,405 + +0.1 3,080,406 - +0.2 3,080,406 - +1.8 3,080,406 - +0.3 3,080,406 - -1.2 3,080,406 - +0.6 3,080,406 - -0.0 3,080,478 + +0.2 3,080,580 + -1.0 3,080,581 - +0.2 3,080,727 + Pf1N1B4_2742 0.19 +1.1 3,080,770 + Pf1N1B4_2742 0.24 +1.2 3,080,771 - Pf1N1B4_2742 0.24 -1.4 3,080,771 - Pf1N1B4_2742 0.24 +0.2 3,080,771 - Pf1N1B4_2742 0.24 +1.6 3,080,771 - Pf1N1B4_2742 0.24 -0.4 3,080,774 + Pf1N1B4_2742 0.24 -0.4 3,080,774 + Pf1N1B4_2742 0.24 -1.0 3,080,774 + Pf1N1B4_2742 0.24 -0.4 3,080,774 + Pf1N1B4_2742 0.24 -0.8 3,080,775 - Pf1N1B4_2742 0.24 -0.8 3,080,775 - Pf1N1B4_2742 0.24 +0.2 3,080,775 - Pf1N1B4_2742 0.24 -0.8 3,080,775 - Pf1N1B4_2742 0.24 +0.4 3,080,795 + Pf1N1B4_2742 0.26 -0.3 3,080,795 + Pf1N1B4_2742 0.26 +1.6 3,080,795 + Pf1N1B4_2742 0.26 -0.2 3,080,834 + Pf1N1B4_2742 0.30 -0.2 3,080,834 + Pf1N1B4_2742 0.30 -1.0 3,080,835 - Pf1N1B4_2742 0.31 +1.4 3,080,859 - Pf1N1B4_2742 0.33 +0.9 3,080,859 - Pf1N1B4_2742 0.33 -1.5 3,080,924 + Pf1N1B4_2742 0.40 +1.0 3,080,924 + Pf1N1B4_2742 0.40 -0.6 3,080,924 + Pf1N1B4_2742 0.40 -2.0 3,080,924 + Pf1N1B4_2742 0.40 -0.4 3,080,924 + Pf1N1B4_2742 0.40 -0.5 3,080,924 + Pf1N1B4_2742 0.40 -1.6 3,080,924 + Pf1N1B4_2742 0.40 -0.3 3,080,925 - Pf1N1B4_2742 0.40 +0.3 3,080,925 - Pf1N1B4_2742 0.40 +2.3 3,080,925 - Pf1N1B4_2742 0.40 -0.0 3,080,925 - Pf1N1B4_2742 0.40 +0.6 3,080,925 - Pf1N1B4_2742 0.40 +0.5 3,080,930 + Pf1N1B4_2742 0.40 +0.6 3,080,930 + Pf1N1B4_2742 0.40 +0.4 3,080,930 + Pf1N1B4_2742 0.40 -0.8 3,080,931 - Pf1N1B4_2742 0.41 -2.8 3,080,931 - Pf1N1B4_2742 0.41 +0.6 3,080,931 - Pf1N1B4_2742 0.41 +0.3 3,080,954 + Pf1N1B4_2742 0.43 +0.1 3,080,954 + Pf1N1B4_2742 0.43 -0.9 3,080,954 + Pf1N1B4_2742 0.43 +0.1 3,080,955 - Pf1N1B4_2742 0.43 -0.9 3,080,955 - Pf1N1B4_2742 0.43 -0.4 3,081,136 - Pf1N1B4_2742 0.62 -2.4 3,081,188 - Pf1N1B4_2742 0.67 +0.6 3,081,188 - Pf1N1B4_2742 0.67 +0.2 3,081,308 + Pf1N1B4_2742 0.80 +1.2 3,081,309 - -0.8
Or see this region's nucleotide sequence