Experiment: Cobalt chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1872 and Pf1N1B4_1873 are separated by 118 nucleotides Pf1N1B4_1873 and Pf1N1B4_1874 are separated by 59 nucleotides Pf1N1B4_1874 and Pf1N1B4_1875 are separated by 74 nucleotides
Pf1N1B4_1872 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 2,141,599 to 2,142,528
_1872
Pf1N1B4_1873 - Homoserine kinase (EC 2.7.1.39), at 2,142,647 to 2,143,597
_1873
Pf1N1B4_1874 - FIG00953549: hypothetical protein, at 2,143,657 to 2,143,944
_1874
Pf1N1B4_1875 - DNA polymerase I (EC 2.7.7.7), at 2,144,019 to 2,146,787
_1875
Position (kb)
2142
2143
2144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2141.741 kb on + strand, within Pf1N1B4_1872 at 2141.784 kb on + strand, within Pf1N1B4_1872 at 2141.785 kb on - strand, within Pf1N1B4_1872 at 2141.785 kb on - strand, within Pf1N1B4_1872 at 2141.785 kb on - strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.788 kb on + strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.789 kb on - strand, within Pf1N1B4_1872 at 2141.810 kb on + strand, within Pf1N1B4_1872 at 2141.810 kb on + strand, within Pf1N1B4_1872 at 2141.810 kb on + strand, within Pf1N1B4_1872 at 2141.810 kb on + strand, within Pf1N1B4_1872 at 2141.811 kb on - strand, within Pf1N1B4_1872 at 2141.811 kb on - strand, within Pf1N1B4_1872 at 2141.892 kb on + strand, within Pf1N1B4_1872 at 2141.893 kb on - strand, within Pf1N1B4_1872 at 2142.155 kb on + strand, within Pf1N1B4_1872 at 2142.155 kb on + strand, within Pf1N1B4_1872 at 2142.202 kb on + strand at 2142.202 kb on + strand, within Pf1N1B4_1872 at 2142.202 kb on + strand, within Pf1N1B4_1872 at 2142.202 kb on + strand, within Pf1N1B4_1872 at 2142.202 kb on + strand, within Pf1N1B4_1872 at 2142.203 kb on - strand, within Pf1N1B4_1872 at 2142.203 kb on - strand, within Pf1N1B4_1872 at 2142.229 kb on + strand, within Pf1N1B4_1872 at 2142.266 kb on + strand, within Pf1N1B4_1872 at 2142.267 kb on - strand, within Pf1N1B4_1872 at 2142.287 kb on + strand, within Pf1N1B4_1872 at 2142.287 kb on + strand, within Pf1N1B4_1872 at 2142.287 kb on + strand, within Pf1N1B4_1872 at 2142.287 kb on + strand at 2142.470 kb on - strand at 2142.470 kb on - strand at 2142.526 kb on + strand at 2142.526 kb on + strand at 2142.526 kb on + strand at 2142.526 kb on + strand at 2142.526 kb on + strand at 2142.527 kb on - strand at 2142.527 kb on - strand at 2142.527 kb on - strand at 2142.527 kb on - strand at 2142.527 kb on - strand at 2142.585 kb on - strand at 2142.585 kb on - strand at 2142.600 kb on + strand at 2142.600 kb on + strand at 2142.600 kb on + strand at 2142.600 kb on + strand at 2142.601 kb on - strand at 2142.648 kb on + strand at 2142.649 kb on - strand at 2142.649 kb on - strand at 2143.643 kb on - strand at 2143.643 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.659 kb on - strand at 2143.670 kb on - strand at 2143.752 kb on - strand, within Pf1N1B4_1874 at 2143.752 kb on - strand, within Pf1N1B4_1874 at 2143.752 kb on - strand, within Pf1N1B4_1874 at 2143.752 kb on - strand at 2143.788 kb on - strand, within Pf1N1B4_1874 at 2143.846 kb on + strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.933 kb on + strand at 2143.934 kb on - strand at 2143.947 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.3125 mM remove 2,141,741 + Pf1N1B4_1872 0.15 -0.5 2,141,784 + Pf1N1B4_1872 0.20 -0.2 2,141,785 - Pf1N1B4_1872 0.20 -3.1 2,141,785 - Pf1N1B4_1872 0.20 -0.2 2,141,785 - Pf1N1B4_1872 0.20 +0.0 2,141,788 + Pf1N1B4_1872 0.20 -0.2 2,141,788 + Pf1N1B4_1872 0.20 +1.2 2,141,788 + Pf1N1B4_1872 0.20 -1.6 2,141,788 + Pf1N1B4_1872 0.20 -0.4 2,141,788 + Pf1N1B4_1872 0.20 +0.9 2,141,788 + Pf1N1B4_1872 0.20 +0.6 2,141,788 + Pf1N1B4_1872 0.20 -0.6 2,141,788 + Pf1N1B4_1872 0.20 +0.1 2,141,788 + Pf1N1B4_1872 0.20 +0.9 2,141,789 - Pf1N1B4_1872 0.20 +1.2 2,141,789 - Pf1N1B4_1872 0.20 -1.2 2,141,789 - Pf1N1B4_1872 0.20 +0.5 2,141,789 - Pf1N1B4_1872 0.20 -0.5 2,141,789 - Pf1N1B4_1872 0.20 +0.0 2,141,789 - Pf1N1B4_1872 0.20 -0.9 2,141,789 - Pf1N1B4_1872 0.20 +0.1 2,141,789 - Pf1N1B4_1872 0.20 +1.4 2,141,789 - Pf1N1B4_1872 0.20 -0.4 2,141,789 - Pf1N1B4_1872 0.20 +0.8 2,141,789 - Pf1N1B4_1872 0.20 -1.8 2,141,789 - Pf1N1B4_1872 0.20 +1.4 2,141,810 + Pf1N1B4_1872 0.23 +1.2 2,141,810 + Pf1N1B4_1872 0.23 +0.1 2,141,810 + Pf1N1B4_1872 0.23 +0.4 2,141,810 + Pf1N1B4_1872 0.23 +0.7 2,141,811 - Pf1N1B4_1872 0.23 +0.1 2,141,811 - Pf1N1B4_1872 0.23 +0.7 2,141,892 + Pf1N1B4_1872 0.32 +0.6 2,141,893 - Pf1N1B4_1872 0.32 +0.2 2,142,155 + Pf1N1B4_1872 0.60 +0.2 2,142,155 + Pf1N1B4_1872 0.60 +3.4 2,142,202 + +1.2 2,142,202 + Pf1N1B4_1872 0.65 +1.6 2,142,202 + Pf1N1B4_1872 0.65 +0.1 2,142,202 + Pf1N1B4_1872 0.65 +3.4 2,142,202 + Pf1N1B4_1872 0.65 -0.3 2,142,203 - Pf1N1B4_1872 0.65 +0.8 2,142,203 - Pf1N1B4_1872 0.65 +0.5 2,142,229 + Pf1N1B4_1872 0.68 -1.0 2,142,266 + Pf1N1B4_1872 0.72 +1.2 2,142,267 - Pf1N1B4_1872 0.72 -2.3 2,142,287 + Pf1N1B4_1872 0.74 +1.0 2,142,287 + Pf1N1B4_1872 0.74 -0.8 2,142,287 + Pf1N1B4_1872 0.74 -0.2 2,142,287 + -1.2 2,142,470 - +1.5 2,142,470 - +1.1 2,142,526 + -0.4 2,142,526 + +0.2 2,142,526 + -0.1 2,142,526 + -1.0 2,142,526 + -0.9 2,142,527 - -0.1 2,142,527 - -0.3 2,142,527 - -0.4 2,142,527 - +0.9 2,142,527 - +0.2 2,142,585 - -0.2 2,142,585 - +1.4 2,142,600 + -0.2 2,142,600 + +0.8 2,142,600 + -0.6 2,142,600 + +1.1 2,142,601 - +0.1 2,142,648 + -1.9 2,142,649 - +0.6 2,142,649 - +0.2 2,143,643 - -0.8 2,143,643 - +0.6 2,143,648 - -0.2 2,143,648 - -1.3 2,143,648 - +0.2 2,143,648 - +0.0 2,143,648 - +1.1 2,143,659 - -0.2 2,143,670 - -1.6 2,143,752 - Pf1N1B4_1874 0.33 -0.5 2,143,752 - Pf1N1B4_1874 0.33 +0.8 2,143,752 - Pf1N1B4_1874 0.33 -0.4 2,143,752 - -0.2 2,143,788 - Pf1N1B4_1874 0.45 -0.6 2,143,846 + Pf1N1B4_1874 0.66 +1.3 2,143,847 - Pf1N1B4_1874 0.66 +0.4 2,143,847 - Pf1N1B4_1874 0.66 -0.4 2,143,847 - Pf1N1B4_1874 0.66 -1.6 2,143,847 - Pf1N1B4_1874 0.66 +0.5 2,143,847 - +0.2 2,143,847 - Pf1N1B4_1874 0.66 +0.5 2,143,847 - Pf1N1B4_1874 0.66 +0.6 2,143,847 - Pf1N1B4_1874 0.66 -0.1 2,143,847 - Pf1N1B4_1874 0.66 +0.8 2,143,933 + +1.1 2,143,934 - +0.2 2,143,947 + +1.1
Or see this region's nucleotide sequence