Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32350 and BPHYT_RS32355 are separated by 14 nucleotides BPHYT_RS32355 and BPHYT_RS32360 overlap by 14 nucleotides BPHYT_RS32360 and BPHYT_RS32365 are separated by 72 nucleotides
BPHYT_RS32350: BPHYT_RS32350 - ABC transporter permease, at 2,829,346 to 2,830,158
_RS32350
BPHYT_RS32355: BPHYT_RS32355 - ABC transporter permease, at 2,830,173 to 2,831,045
_RS32355
BPHYT_RS32360: BPHYT_RS32360 - spermidine/putrescine ABC transporter ATPase, at 2,831,032 to 2,832,120
_RS32360
BPHYT_RS32365: BPHYT_RS32365 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,832,193 to 2,833,260
_RS32365
Position (kb)
2831
2832
2833 Strain fitness (log2 ratio)
-1
0
1 at 2830.113 kb on + strand at 2830.992 kb on + strand at 2831.339 kb on - strand, within BPHYT_RS32360 at 2832.096 kb on + strand at 2832.141 kb on - strand at 2832.402 kb on - strand, within BPHYT_RS32365 at 2832.503 kb on - strand, within BPHYT_RS32365 at 2832.880 kb on + strand, within BPHYT_RS32365 at 2832.880 kb on + strand, within BPHYT_RS32365 at 2832.897 kb on - strand, within BPHYT_RS32365
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,830,113 + +0.1 2,830,992 + -0.7 2,831,339 - BPHYT_RS32360 0.28 +0.3 2,832,096 + -0.2 2,832,141 - -0.5 2,832,402 - BPHYT_RS32365 0.20 -0.6 2,832,503 - BPHYT_RS32365 0.29 +0.5 2,832,880 + BPHYT_RS32365 0.64 -0.2 2,832,880 + BPHYT_RS32365 0.64 +0.2 2,832,897 - BPHYT_RS32365 0.66 -0.2
Or see this region's nucleotide sequence