Experiment: Nickel (II) chloride 0.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25705 and AO356_25710 are separated by 30 nucleotides AO356_25710 and AO356_25715 are separated by 44 nucleotides AO356_25715 and AO356_25720 are separated by 17 nucleotides AO356_25720 and AO356_25725 are separated by 6 nucleotides
AO356_25705: AO356_25705 - monooxygenase, at 5,815,053 to 5,816,096
_25705
AO356_25710: AO356_25710 - flavin reductase, at 5,816,127 to 5,816,612
_25710
AO356_25715: AO356_25715 - branched-chain amino acid ABC transporter substrate-binding protein, at 5,816,657 to 5,817,808
_25715
AO356_25720: AO356_25720 - polyamine ABC transporter permease, at 5,817,826 to 5,818,635
_25720
AO356_25725: AO356_25725 - ABC transporter permease, at 5,818,642 to 5,819,571
_25725
Position (kb)
5816
5817
5818 Strain fitness (log2 ratio)
-1
0
1 at 5815.948 kb on + strand, within AO356_25705 at 5815.949 kb on - strand, within AO356_25705 at 5815.949 kb on - strand, within AO356_25705 at 5816.077 kb on - strand at 5816.077 kb on - strand at 5816.110 kb on + strand at 5816.110 kb on + strand at 5816.111 kb on - strand at 5816.168 kb on - strand at 5816.369 kb on - strand, within AO356_25710 at 5816.369 kb on - strand, within AO356_25710 at 5816.369 kb on - strand, within AO356_25710 at 5816.452 kb on + strand, within AO356_25710 at 5816.452 kb on + strand, within AO356_25710 at 5816.452 kb on + strand, within AO356_25710 at 5816.593 kb on + strand at 5816.594 kb on - strand at 5816.707 kb on - strand at 5816.707 kb on - strand at 5817.109 kb on - strand, within AO356_25715 at 5817.324 kb on + strand, within AO356_25715 at 5817.563 kb on + strand, within AO356_25715 at 5818.259 kb on + strand, within AO356_25720 at 5818.260 kb on - strand, within AO356_25720 at 5818.260 kb on - strand, within AO356_25720 at 5818.691 kb on + strand at 5818.692 kb on - strand at 5818.692 kb on - strand at 5818.697 kb on + strand at 5818.697 kb on + strand at 5818.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.625 mM remove 5,815,948 + AO356_25705 0.86 -0.5 5,815,949 - AO356_25705 0.86 -1.0 5,815,949 - AO356_25705 0.86 -0.3 5,816,077 - +0.9 5,816,077 - -0.7 5,816,110 + -1.1 5,816,110 + -1.4 5,816,111 - -0.4 5,816,168 - +0.3 5,816,369 - AO356_25710 0.50 -0.0 5,816,369 - AO356_25710 0.50 +1.5 5,816,369 - AO356_25710 0.50 +0.1 5,816,452 + AO356_25710 0.67 +0.2 5,816,452 + AO356_25710 0.67 -1.6 5,816,452 + AO356_25710 0.67 +0.1 5,816,593 + -0.0 5,816,594 - -0.7 5,816,707 - -0.3 5,816,707 - +0.8 5,817,109 - AO356_25715 0.39 +0.6 5,817,324 + AO356_25715 0.58 +1.6 5,817,563 + AO356_25715 0.79 +0.2 5,818,259 + AO356_25720 0.53 -0.2 5,818,260 - AO356_25720 0.54 +0.8 5,818,260 - AO356_25720 0.54 +0.5 5,818,691 + -0.1 5,818,692 - +0.2 5,818,692 - +0.0 5,818,697 + +0.1 5,818,697 + +0.4 5,818,698 - +0.8
Or see this region's nucleotide sequence